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Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha

Kiwifruit (Actinidia eriantha) is a dioecious vine, and the pollen of its male cultivars has a direct effect on the quality of its fruits. In this study, to facilitate molecular breeding and gene identification, we performed genome-wide association studies (GWAS) on 11 traits of flower and leaf. A t...

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Autores principales: Liao, Guanglian, Zhong, Min, Jiang, Zhiqiang, Tao, Junjie, Jia, Dongfeng, Qu, Xueyan, Huang, Chunhui, Liu, Qing, Xu, Xiaobiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417775/
https://www.ncbi.nlm.nih.gov/pubmed/34490026
http://dx.doi.org/10.3389/fpls.2021.730890
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author Liao, Guanglian
Zhong, Min
Jiang, Zhiqiang
Tao, Junjie
Jia, Dongfeng
Qu, Xueyan
Huang, Chunhui
Liu, Qing
Xu, Xiaobiao
author_facet Liao, Guanglian
Zhong, Min
Jiang, Zhiqiang
Tao, Junjie
Jia, Dongfeng
Qu, Xueyan
Huang, Chunhui
Liu, Qing
Xu, Xiaobiao
author_sort Liao, Guanglian
collection PubMed
description Kiwifruit (Actinidia eriantha) is a dioecious vine, and the pollen of its male cultivars has a direct effect on the quality of its fruits. In this study, to facilitate molecular breeding and gene identification, we performed genome-wide association studies (GWAS) on 11 traits of flower and leaf. A total of 946,337 highly consistent SNP markers were obtained in the whole genome. Phylogenetic tree analysis and population structure analysis showed that the 143 germplasms can be divided into two groups. The linkage disequilibrium analysis showed that A. eriantha have a relatively fast attenuation rate, and that the average attenuation distance of LD was 0.1–0.3 Kb. The MLM (QK) model was determined as best for correlation analysis, and eight and three SNPs associated with flower- and leaf-related traits were identified, respectively, at 0.01 significance level. However, SNP markers associated with stamen number per flower, pollen viability, total chlorophyll content, and total flavonoid content were not identified at the 0.01 significant level, although it is worth noting that one, one, five, and two SNPs were identified to be associated with these traits at the 0.05 significant level. This study provides insights into the complex flower- and leaf-related biology, and identifies genes controlling important traits in A. eriantha through GWAS, which extends the genetic resources and basis for facilitating molecular breeding in kiwifruits.
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spelling pubmed-84177752021-09-05 Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha Liao, Guanglian Zhong, Min Jiang, Zhiqiang Tao, Junjie Jia, Dongfeng Qu, Xueyan Huang, Chunhui Liu, Qing Xu, Xiaobiao Front Plant Sci Plant Science Kiwifruit (Actinidia eriantha) is a dioecious vine, and the pollen of its male cultivars has a direct effect on the quality of its fruits. In this study, to facilitate molecular breeding and gene identification, we performed genome-wide association studies (GWAS) on 11 traits of flower and leaf. A total of 946,337 highly consistent SNP markers were obtained in the whole genome. Phylogenetic tree analysis and population structure analysis showed that the 143 germplasms can be divided into two groups. The linkage disequilibrium analysis showed that A. eriantha have a relatively fast attenuation rate, and that the average attenuation distance of LD was 0.1–0.3 Kb. The MLM (QK) model was determined as best for correlation analysis, and eight and three SNPs associated with flower- and leaf-related traits were identified, respectively, at 0.01 significance level. However, SNP markers associated with stamen number per flower, pollen viability, total chlorophyll content, and total flavonoid content were not identified at the 0.01 significant level, although it is worth noting that one, one, five, and two SNPs were identified to be associated with these traits at the 0.05 significant level. This study provides insights into the complex flower- and leaf-related biology, and identifies genes controlling important traits in A. eriantha through GWAS, which extends the genetic resources and basis for facilitating molecular breeding in kiwifruits. Frontiers Media S.A. 2021-08-20 /pmc/articles/PMC8417775/ /pubmed/34490026 http://dx.doi.org/10.3389/fpls.2021.730890 Text en Copyright © 2021 Liao, Zhong, Jiang, Tao, Jia, Qu, Huang, Liu and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liao, Guanglian
Zhong, Min
Jiang, Zhiqiang
Tao, Junjie
Jia, Dongfeng
Qu, Xueyan
Huang, Chunhui
Liu, Qing
Xu, Xiaobiao
Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title_full Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title_fullStr Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title_full_unstemmed Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title_short Genome-Wide Association Studies Provide Insights into the Genetic Determination of Flower and Leaf Traits of Actinidia eriantha
title_sort genome-wide association studies provide insights into the genetic determination of flower and leaf traits of actinidia eriantha
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417775/
https://www.ncbi.nlm.nih.gov/pubmed/34490026
http://dx.doi.org/10.3389/fpls.2021.730890
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