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Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus

Patients with liver disease are susceptible to infection with Vibrio vulnificus (V. vulnificus), but the specific reasons remain elusive. Through RNA-seq, we found that when mice with alcoholic liver disease (ALD) were infected with V. vulnificus by gavage, compared with the Pair group, the small in...

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Autores principales: Feng, Zi-Han, Li, Shi-Qing, Zhang, Jia-Xin, Ni, Bin, Bai, Xin-Ru, Xu, Jian-Hao, Liu, Zhen-Bo, Xin, Wen-Wen, Kang, Lin, Gao, Shan, Wang, Jing, Li, Yan-Wei, Li, Jia-Xin, Yuan, Yuan, Wang, Jing-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417779/
https://www.ncbi.nlm.nih.gov/pubmed/34489943
http://dx.doi.org/10.3389/fimmu.2021.695491
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author Feng, Zi-Han
Li, Shi-Qing
Zhang, Jia-Xin
Ni, Bin
Bai, Xin-Ru
Xu, Jian-Hao
Liu, Zhen-Bo
Xin, Wen-Wen
Kang, Lin
Gao, Shan
Wang, Jing
Li, Yan-Wei
Li, Jia-Xin
Yuan, Yuan
Wang, Jing-Lin
author_facet Feng, Zi-Han
Li, Shi-Qing
Zhang, Jia-Xin
Ni, Bin
Bai, Xin-Ru
Xu, Jian-Hao
Liu, Zhen-Bo
Xin, Wen-Wen
Kang, Lin
Gao, Shan
Wang, Jing
Li, Yan-Wei
Li, Jia-Xin
Yuan, Yuan
Wang, Jing-Lin
author_sort Feng, Zi-Han
collection PubMed
description Patients with liver disease are susceptible to infection with Vibrio vulnificus (V. vulnificus), but the specific reasons remain elusive. Through RNA-seq, we found that when mice with alcoholic liver disease (ALD) were infected with V. vulnificus by gavage, compared with the Pair group, the small intestinal genes affecting intestinal permeability were upregulated; and the number of differentially expressed genes related to immune functions (e.g., such as cell chemotaxis, leukocyte differentiation, and neutrophil degranulation) decreased in the liver, spleen, and blood. Further analysis showed that the number of white blood cells decreased in the Pair group, whereas those in the ALD mice did not change significantly. Interestingly, the blood bacterial load in the ALD mice was about 100 times higher than that of the Pair group. After the ALD mice were infected with V. vulnificus, the concentrations of T cell proliferation-promoting cytokines (IL-2, IL-23) decreased. Therefore, unlike the Pair group, ALD mice had weaker immune responses, lower T cell proliferation-promoting cytokines, and higher bacterial loads post-infection, possibly increasing their susceptibility to V. vulnificus infection. These new findings we presented here may help to advance the current understanding of the reasons why patients with liver disease are susceptible to V. vulnificus infection and provides potential targets for further investigation in the context of treatment options for V. vulnificus sepsis in liver disease patient.
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spelling pubmed-84177792021-09-05 Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus Feng, Zi-Han Li, Shi-Qing Zhang, Jia-Xin Ni, Bin Bai, Xin-Ru Xu, Jian-Hao Liu, Zhen-Bo Xin, Wen-Wen Kang, Lin Gao, Shan Wang, Jing Li, Yan-Wei Li, Jia-Xin Yuan, Yuan Wang, Jing-Lin Front Immunol Immunology Patients with liver disease are susceptible to infection with Vibrio vulnificus (V. vulnificus), but the specific reasons remain elusive. Through RNA-seq, we found that when mice with alcoholic liver disease (ALD) were infected with V. vulnificus by gavage, compared with the Pair group, the small intestinal genes affecting intestinal permeability were upregulated; and the number of differentially expressed genes related to immune functions (e.g., such as cell chemotaxis, leukocyte differentiation, and neutrophil degranulation) decreased in the liver, spleen, and blood. Further analysis showed that the number of white blood cells decreased in the Pair group, whereas those in the ALD mice did not change significantly. Interestingly, the blood bacterial load in the ALD mice was about 100 times higher than that of the Pair group. After the ALD mice were infected with V. vulnificus, the concentrations of T cell proliferation-promoting cytokines (IL-2, IL-23) decreased. Therefore, unlike the Pair group, ALD mice had weaker immune responses, lower T cell proliferation-promoting cytokines, and higher bacterial loads post-infection, possibly increasing their susceptibility to V. vulnificus infection. These new findings we presented here may help to advance the current understanding of the reasons why patients with liver disease are susceptible to V. vulnificus infection and provides potential targets for further investigation in the context of treatment options for V. vulnificus sepsis in liver disease patient. Frontiers Media S.A. 2021-08-20 /pmc/articles/PMC8417779/ /pubmed/34489943 http://dx.doi.org/10.3389/fimmu.2021.695491 Text en Copyright © 2021 Feng, Li, Zhang, Ni, Bai, Xu, Liu, Xin, Kang, Gao, Wang, Li, Li, Yuan and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Feng, Zi-Han
Li, Shi-Qing
Zhang, Jia-Xin
Ni, Bin
Bai, Xin-Ru
Xu, Jian-Hao
Liu, Zhen-Bo
Xin, Wen-Wen
Kang, Lin
Gao, Shan
Wang, Jing
Li, Yan-Wei
Li, Jia-Xin
Yuan, Yuan
Wang, Jing-Lin
Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title_full Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title_fullStr Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title_full_unstemmed Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title_short Analysis of Gene Expression Profiles, Cytokines, and Bacterial Loads Relevant to Alcoholic Liver Disease Mice Infected With V. vulnificus
title_sort analysis of gene expression profiles, cytokines, and bacterial loads relevant to alcoholic liver disease mice infected with v. vulnificus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417779/
https://www.ncbi.nlm.nih.gov/pubmed/34489943
http://dx.doi.org/10.3389/fimmu.2021.695491
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