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Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction

Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental...

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Autores principales: Torres-Silva, Gabriela, Correia, Ludmila Nayara Freitas, Batista, Diego Silva, Koehler, Andréa Dias, Resende, Sheila Vitória, Romanel, Elisson, Cassol, Daniela, Almeida, Ana Maria Rocha, Strickler, Susan R., Specht, Chelsea Dvorak, Otoni, Wagner Campos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417902/
https://www.ncbi.nlm.nih.gov/pubmed/34490003
http://dx.doi.org/10.3389/fpls.2021.697556
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author Torres-Silva, Gabriela
Correia, Ludmila Nayara Freitas
Batista, Diego Silva
Koehler, Andréa Dias
Resende, Sheila Vitória
Romanel, Elisson
Cassol, Daniela
Almeida, Ana Maria Rocha
Strickler, Susan R.
Specht, Chelsea Dvorak
Otoni, Wagner Campos
author_facet Torres-Silva, Gabriela
Correia, Ludmila Nayara Freitas
Batista, Diego Silva
Koehler, Andréa Dias
Resende, Sheila Vitória
Romanel, Elisson
Cassol, Daniela
Almeida, Ana Maria Rocha
Strickler, Susan R.
Specht, Chelsea Dvorak
Otoni, Wagner Campos
author_sort Torres-Silva, Gabriela
collection PubMed
description Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental processes were known. The present study generated de novo transcriptome data, describing in vitro shoot organogenesis induction in M. glaucescens. Total RNA was extracted from explants before (control) and after shoot organogenesis induction (treated). A total of 14,478 unigenes (average length, 520 bases) were obtained using Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) sequencing and transcriptome assembly. Filtering for differential expression yielded 2,058 unigenes. Pairwise comparison of treated vs. control genes revealed that 1,241 (60.3%) unigenes exhibited no significant change, 226 (11%) were downregulated, and 591 (28.7%) were upregulated. Based on database analysis, more transcription factor families and unigenes appeared to be upregulated in the treated samples than in controls. Expression of WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and CALMODULIN (CaM) genes, both of which were upregulated in treated samples, was further validated by real-time quantitative PCR (RT-qPCR). Differences in gene expression patterns between control and treated samples indicate substantial changes in the primary and secondary metabolism of M. glaucescens after the induction of shoot organogenesis. These results help to clarify the molecular genetics and functional genomic aspects underlying propagation in the Cactaceae family.
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spelling pubmed-84179022021-09-05 Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction Torres-Silva, Gabriela Correia, Ludmila Nayara Freitas Batista, Diego Silva Koehler, Andréa Dias Resende, Sheila Vitória Romanel, Elisson Cassol, Daniela Almeida, Ana Maria Rocha Strickler, Susan R. Specht, Chelsea Dvorak Otoni, Wagner Campos Front Plant Sci Plant Science Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental processes were known. The present study generated de novo transcriptome data, describing in vitro shoot organogenesis induction in M. glaucescens. Total RNA was extracted from explants before (control) and after shoot organogenesis induction (treated). A total of 14,478 unigenes (average length, 520 bases) were obtained using Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) sequencing and transcriptome assembly. Filtering for differential expression yielded 2,058 unigenes. Pairwise comparison of treated vs. control genes revealed that 1,241 (60.3%) unigenes exhibited no significant change, 226 (11%) were downregulated, and 591 (28.7%) were upregulated. Based on database analysis, more transcription factor families and unigenes appeared to be upregulated in the treated samples than in controls. Expression of WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and CALMODULIN (CaM) genes, both of which were upregulated in treated samples, was further validated by real-time quantitative PCR (RT-qPCR). Differences in gene expression patterns between control and treated samples indicate substantial changes in the primary and secondary metabolism of M. glaucescens after the induction of shoot organogenesis. These results help to clarify the molecular genetics and functional genomic aspects underlying propagation in the Cactaceae family. Frontiers Media S.A. 2021-08-20 /pmc/articles/PMC8417902/ /pubmed/34490003 http://dx.doi.org/10.3389/fpls.2021.697556 Text en Copyright © 2021 Torres-Silva, Correia, Batista, Koehler, Resende, Romanel, Cassol, Almeida, Strickler, Specht and Otoni. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Torres-Silva, Gabriela
Correia, Ludmila Nayara Freitas
Batista, Diego Silva
Koehler, Andréa Dias
Resende, Sheila Vitória
Romanel, Elisson
Cassol, Daniela
Almeida, Ana Maria Rocha
Strickler, Susan R.
Specht, Chelsea Dvorak
Otoni, Wagner Campos
Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title_full Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title_fullStr Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title_full_unstemmed Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title_short Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction
title_sort transcriptome analysis of melocactus glaucescens (cactaceae) reveals metabolic changes during in vitro shoot organogenesis induction
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417902/
https://www.ncbi.nlm.nih.gov/pubmed/34490003
http://dx.doi.org/10.3389/fpls.2021.697556
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