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Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance
Root rot of Populus davidiana × P. alba var. pyramidalis Louche (Pdpap) is caused by Fusarium oxysporum. We used RNA sequencing to study the molecular mechanisms and response pattern of Pdpap infected by F. oxysporum CFCC86068. We cloned the PdpapWRKY28 transcription factor gene and transformed the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418079/ https://www.ncbi.nlm.nih.gov/pubmed/34490017 http://dx.doi.org/10.3389/fpls.2021.720679 |
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author | Diao, Jian Wang, Jiaqi Zhang, Ping Hao, Xin Wang, Yang Liang, Liwei Zhang, Yue Ma, Wei Ma, Ling |
author_facet | Diao, Jian Wang, Jiaqi Zhang, Ping Hao, Xin Wang, Yang Liang, Liwei Zhang, Yue Ma, Wei Ma, Ling |
author_sort | Diao, Jian |
collection | PubMed |
description | Root rot of Populus davidiana × P. alba var. pyramidalis Louche (Pdpap) is caused by Fusarium oxysporum. We used RNA sequencing to study the molecular mechanisms and response pattern of Pdpap infected by F. oxysporum CFCC86068. We cloned the PdpapWRKY28 transcription factor gene and transformed the recombinant vector pBI121-PdpapWRKY28 into Pdpap. The resistance function of PdpapWRKY28 was verified using physiological and biochemical methods. By means of RNA sequencing, we detected 1,403 differentially expressed genes (DEGs) that are common in the different treatments by F. oxysporum. Furthermore, we found that overexpression of the PdpapWRKY28 gene may significantly improve the resistance of Pdpap plants to F. oxysporum. Our research reveals a key role for PdpapWRKY28 in the resistance response of Pdpap to F. oxysporum. Additionally, our results provide a theoretical basis for in-depth research on resistance breeding to combat root rot. |
format | Online Article Text |
id | pubmed-8418079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84180792021-09-05 Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance Diao, Jian Wang, Jiaqi Zhang, Ping Hao, Xin Wang, Yang Liang, Liwei Zhang, Yue Ma, Wei Ma, Ling Front Plant Sci Plant Science Root rot of Populus davidiana × P. alba var. pyramidalis Louche (Pdpap) is caused by Fusarium oxysporum. We used RNA sequencing to study the molecular mechanisms and response pattern of Pdpap infected by F. oxysporum CFCC86068. We cloned the PdpapWRKY28 transcription factor gene and transformed the recombinant vector pBI121-PdpapWRKY28 into Pdpap. The resistance function of PdpapWRKY28 was verified using physiological and biochemical methods. By means of RNA sequencing, we detected 1,403 differentially expressed genes (DEGs) that are common in the different treatments by F. oxysporum. Furthermore, we found that overexpression of the PdpapWRKY28 gene may significantly improve the resistance of Pdpap plants to F. oxysporum. Our research reveals a key role for PdpapWRKY28 in the resistance response of Pdpap to F. oxysporum. Additionally, our results provide a theoretical basis for in-depth research on resistance breeding to combat root rot. Frontiers Media S.A. 2021-08-20 /pmc/articles/PMC8418079/ /pubmed/34490017 http://dx.doi.org/10.3389/fpls.2021.720679 Text en Copyright © 2021 Diao, Wang, Zhang, Hao, Wang, Liang, Zhang, Ma and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Diao, Jian Wang, Jiaqi Zhang, Ping Hao, Xin Wang, Yang Liang, Liwei Zhang, Yue Ma, Wei Ma, Ling Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title | Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title_full | Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title_fullStr | Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title_full_unstemmed | Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title_short | Transcriptome Analysis Reveals the Important Role of WRKY28 in Fusarium oxysporum Resistance |
title_sort | transcriptome analysis reveals the important role of wrky28 in fusarium oxysporum resistance |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418079/ https://www.ncbi.nlm.nih.gov/pubmed/34490017 http://dx.doi.org/10.3389/fpls.2021.720679 |
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