Cargando…
A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis)
Bovine tuberculosis (bTB) remains endemic in domestic water buffaloes (Bubalus bubalis) in India and elsewhere, with limited options for control other than testing and slaughter. The prescribed tuberculin skin tests with purified protein derivative (PPD) for diagnosis of bTB preclude the use of Baci...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418101/ https://www.ncbi.nlm.nih.gov/pubmed/34490387 http://dx.doi.org/10.3389/fvets.2021.669898 |
_version_ | 1783748514292957184 |
---|---|
author | Kumar, Tarun Singh, Mahavir Jangir, Babu Lal Arora, Devan Srinivasan, Sreenidhi Bidhan, Devender Yadav, Dipin Chander Veerasami, Maroudam Bakker, Douwe Kapur, Vivek Jindal, Naresh |
author_facet | Kumar, Tarun Singh, Mahavir Jangir, Babu Lal Arora, Devan Srinivasan, Sreenidhi Bidhan, Devender Yadav, Dipin Chander Veerasami, Maroudam Bakker, Douwe Kapur, Vivek Jindal, Naresh |
author_sort | Kumar, Tarun |
collection | PubMed |
description | Bovine tuberculosis (bTB) remains endemic in domestic water buffaloes (Bubalus bubalis) in India and elsewhere, with limited options for control other than testing and slaughter. The prescribed tuberculin skin tests with purified protein derivative (PPD) for diagnosis of bTB preclude the use of Bacille Calmette-Guérin (BCG)-based vaccination because of the antigenic cross-reactivity of vaccine strains with Mycobacterium bovis and related pathogenic members of the M. tuberculosis complex (MTBC). For the diagnosis of bTB in domestic water buffaloes, we here assessed a recently described defined-antigen skin test (DST) that comprises overlapping peptides representing the ESAT-6, CFP-10 and Rv3615c antigens, present in disease-causing members of the MTBC but missing in BCG strains. The performance characteristics of three doses (5, 10 or 20 μg/peptide) of the DST were assessed in natural tuberculin skin test reactor (n = 11) and non-reactor (n = 35) water buffaloes at an organized dairy farm in Hisar, India, and results were compared with the single intradermal skin test (SIT) using standard bovine tuberculin (PPD-B). The results showed a dose-dependent response of DST in natural reactor water buffaloes, although the SIT induced a significantly greater (P < 0.001) skin test response than the highest dose of DST used. However, using a cut-off of 2 mm or greater, the 5, 10, and 20 μg DST cocktail correctly classified eight, 10 and all 11 of the SIT-positive reactors, respectively, suggesting that the 20 μg DST cocktail has a diagnostic sensitivity (Se) of 1.0 (95% CI: 0.72–1.0) identical to that of the SIT. Importantly, none of the tested DST doses induced any measurable skin induration responses in the 35 SIT-negative animals, suggesting a specificity point estimate of 1.0 (95% CI: 0.9–1.0), also identical to that of the SIT and compares favorably with that of the comparative cervical test (Se = 0.85; 95% CI: 0.55–0.98). Overall, the results suggest that similar to tuberculin, the DST enables sensitive and specific diagnosis of bTB in water buffaloes. Future field trials to explore the utility of DST as a defined antigen replacement for tuberculin in routine surveillance programs and to enable BCG vaccination of water buffaloes are warranted. |
format | Online Article Text |
id | pubmed-8418101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84181012021-09-05 A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) Kumar, Tarun Singh, Mahavir Jangir, Babu Lal Arora, Devan Srinivasan, Sreenidhi Bidhan, Devender Yadav, Dipin Chander Veerasami, Maroudam Bakker, Douwe Kapur, Vivek Jindal, Naresh Front Vet Sci Veterinary Science Bovine tuberculosis (bTB) remains endemic in domestic water buffaloes (Bubalus bubalis) in India and elsewhere, with limited options for control other than testing and slaughter. The prescribed tuberculin skin tests with purified protein derivative (PPD) for diagnosis of bTB preclude the use of Bacille Calmette-Guérin (BCG)-based vaccination because of the antigenic cross-reactivity of vaccine strains with Mycobacterium bovis and related pathogenic members of the M. tuberculosis complex (MTBC). For the diagnosis of bTB in domestic water buffaloes, we here assessed a recently described defined-antigen skin test (DST) that comprises overlapping peptides representing the ESAT-6, CFP-10 and Rv3615c antigens, present in disease-causing members of the MTBC but missing in BCG strains. The performance characteristics of three doses (5, 10 or 20 μg/peptide) of the DST were assessed in natural tuberculin skin test reactor (n = 11) and non-reactor (n = 35) water buffaloes at an organized dairy farm in Hisar, India, and results were compared with the single intradermal skin test (SIT) using standard bovine tuberculin (PPD-B). The results showed a dose-dependent response of DST in natural reactor water buffaloes, although the SIT induced a significantly greater (P < 0.001) skin test response than the highest dose of DST used. However, using a cut-off of 2 mm or greater, the 5, 10, and 20 μg DST cocktail correctly classified eight, 10 and all 11 of the SIT-positive reactors, respectively, suggesting that the 20 μg DST cocktail has a diagnostic sensitivity (Se) of 1.0 (95% CI: 0.72–1.0) identical to that of the SIT. Importantly, none of the tested DST doses induced any measurable skin induration responses in the 35 SIT-negative animals, suggesting a specificity point estimate of 1.0 (95% CI: 0.9–1.0), also identical to that of the SIT and compares favorably with that of the comparative cervical test (Se = 0.85; 95% CI: 0.55–0.98). Overall, the results suggest that similar to tuberculin, the DST enables sensitive and specific diagnosis of bTB in water buffaloes. Future field trials to explore the utility of DST as a defined antigen replacement for tuberculin in routine surveillance programs and to enable BCG vaccination of water buffaloes are warranted. Frontiers Media S.A. 2021-08-16 /pmc/articles/PMC8418101/ /pubmed/34490387 http://dx.doi.org/10.3389/fvets.2021.669898 Text en Copyright © 2021 Kumar, Singh, Jangir, Arora, Srinivasan, Bidhan, Yadav, Veerasami, Bakker, Kapur and Jindal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Kumar, Tarun Singh, Mahavir Jangir, Babu Lal Arora, Devan Srinivasan, Sreenidhi Bidhan, Devender Yadav, Dipin Chander Veerasami, Maroudam Bakker, Douwe Kapur, Vivek Jindal, Naresh A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title | A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title_full | A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title_fullStr | A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title_full_unstemmed | A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title_short | A Defined Antigen Skin Test for Diagnosis of Bovine Tuberculosis in Domestic Water Buffaloes (Bubalus bubalis) |
title_sort | defined antigen skin test for diagnosis of bovine tuberculosis in domestic water buffaloes (bubalus bubalis) |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418101/ https://www.ncbi.nlm.nih.gov/pubmed/34490387 http://dx.doi.org/10.3389/fvets.2021.669898 |
work_keys_str_mv | AT kumartarun adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT singhmahavir adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT jangirbabulal adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT aroradevan adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT srinivasansreenidhi adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT bidhandevender adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT yadavdipinchander adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT veerasamimaroudam adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT bakkerdouwe adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT kapurvivek adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT jindalnaresh adefinedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT kumartarun definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT singhmahavir definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT jangirbabulal definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT aroradevan definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT srinivasansreenidhi definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT bidhandevender definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT yadavdipinchander definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT veerasamimaroudam definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT bakkerdouwe definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT kapurvivek definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis AT jindalnaresh definedantigenskintestfordiagnosisofbovinetuberculosisindomesticwaterbuffaloesbubalusbubalis |