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A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control
In chloroplasts, thiol-dependent redox regulation is linked to light since the disulfide reductase activity of thioredoxins (Trxs) relies on photo-reduced ferredoxin (Fdx). Furthermore, chloroplasts harbor an NADPH-dependent Trx reductase (NTR) with a joint Trx domain, termed NTRC. The activity of t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418392/ https://www.ncbi.nlm.nih.gov/pubmed/34618130 http://dx.doi.org/10.1093/plphys/kiab246 |
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author | Ojeda, Valle Jiménez-López, Julia Romero-Campero, Francisco José Cejudo, Francisco Javier Pérez-Ruiz, Juan Manuel |
author_facet | Ojeda, Valle Jiménez-López, Julia Romero-Campero, Francisco José Cejudo, Francisco Javier Pérez-Ruiz, Juan Manuel |
author_sort | Ojeda, Valle |
collection | PubMed |
description | In chloroplasts, thiol-dependent redox regulation is linked to light since the disulfide reductase activity of thioredoxins (Trxs) relies on photo-reduced ferredoxin (Fdx). Furthermore, chloroplasts harbor an NADPH-dependent Trx reductase (NTR) with a joint Trx domain, termed NTRC. The activity of these two redox systems is integrated by the redox balance of 2-Cys peroxiredoxin (Prx), which is controlled by NTRC. However, NTRC was proposed to participate in redox regulation of additional targets, prompting inquiry into whether the function of NTRC depends on its capacity to maintain the redox balance of 2-Cys Prxs or by direct redox interaction with chloroplast enzymes. To answer this, we studied the functional relationship of NTRC and 2-Cys Prxs by a comparative analysis of the triple Arabidopsis (Arabidopsis thaliana) mutant, ntrc-2cpab, which lacks NTRC and 2-Cys Prxs, and the double mutant 2cpab, which lacks 2-Cys Prxs. These mutants exhibit almost indistinguishable phenotypes: in growth rate, photosynthesis performance, and redox regulation of chloroplast enzymes in response to light and darkness. These results suggest that the most relevant function of NTRC is in controlling the redox balance of 2-Cys Prxs. A comparative transcriptomics analysis confirmed the phenotypic similarity of the two mutants and suggested that the NTRC-2-Cys Prxs system participates in cytosolic protein quality control. We propose that NTRC and 2-Cys Prxs constitute a redox relay, exclusive to photosynthetic organisms that fine-tunes the redox state of chloroplast enzymes in response to light and affects transduction pathways towards the cytosol. |
format | Online Article Text |
id | pubmed-8418392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-84183922021-09-09 A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control Ojeda, Valle Jiménez-López, Julia Romero-Campero, Francisco José Cejudo, Francisco Javier Pérez-Ruiz, Juan Manuel Plant Physiol Research Articles In chloroplasts, thiol-dependent redox regulation is linked to light since the disulfide reductase activity of thioredoxins (Trxs) relies on photo-reduced ferredoxin (Fdx). Furthermore, chloroplasts harbor an NADPH-dependent Trx reductase (NTR) with a joint Trx domain, termed NTRC. The activity of these two redox systems is integrated by the redox balance of 2-Cys peroxiredoxin (Prx), which is controlled by NTRC. However, NTRC was proposed to participate in redox regulation of additional targets, prompting inquiry into whether the function of NTRC depends on its capacity to maintain the redox balance of 2-Cys Prxs or by direct redox interaction with chloroplast enzymes. To answer this, we studied the functional relationship of NTRC and 2-Cys Prxs by a comparative analysis of the triple Arabidopsis (Arabidopsis thaliana) mutant, ntrc-2cpab, which lacks NTRC and 2-Cys Prxs, and the double mutant 2cpab, which lacks 2-Cys Prxs. These mutants exhibit almost indistinguishable phenotypes: in growth rate, photosynthesis performance, and redox regulation of chloroplast enzymes in response to light and darkness. These results suggest that the most relevant function of NTRC is in controlling the redox balance of 2-Cys Prxs. A comparative transcriptomics analysis confirmed the phenotypic similarity of the two mutants and suggested that the NTRC-2-Cys Prxs system participates in cytosolic protein quality control. We propose that NTRC and 2-Cys Prxs constitute a redox relay, exclusive to photosynthetic organisms that fine-tunes the redox state of chloroplast enzymes in response to light and affects transduction pathways towards the cytosol. Oxford University Press 2021-05-26 /pmc/articles/PMC8418392/ /pubmed/34618130 http://dx.doi.org/10.1093/plphys/kiab246 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Articles Ojeda, Valle Jiménez-López, Julia Romero-Campero, Francisco José Cejudo, Francisco Javier Pérez-Ruiz, Juan Manuel A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title | A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title_full | A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title_fullStr | A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title_full_unstemmed | A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title_short | A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
title_sort | chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418392/ https://www.ncbi.nlm.nih.gov/pubmed/34618130 http://dx.doi.org/10.1093/plphys/kiab246 |
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