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A microProtein repressor complex in the shoot meristem controls the transition to flowering

MicroProteins are potent post-translational regulators. In Arabidopsis (Arabidopsis thaliana), the miP1a/b microProteins delay floral transition by forming a complex with CONSTANS (CO) and the co-repressor protein TOPLESS. To better understand the function of the miP1a microProtein in floral repress...

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Autores principales: Rodrigues, Vandasue L., Dolde, Ulla, Sun, Bin, Blaakmeer, Anko, Straub, Daniel, Eguen, Tenai, Botterweg-Paredes, Esther, Hong, Shinyoung, Graeff, Moritz, Li, Man-Wah, Gendron, Joshua M., Wenkel, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418433/
https://www.ncbi.nlm.nih.gov/pubmed/34015131
http://dx.doi.org/10.1093/plphys/kiab235
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author Rodrigues, Vandasue L.
Dolde, Ulla
Sun, Bin
Blaakmeer, Anko
Straub, Daniel
Eguen, Tenai
Botterweg-Paredes, Esther
Hong, Shinyoung
Graeff, Moritz
Li, Man-Wah
Gendron, Joshua M.
Wenkel, Stephan
author_facet Rodrigues, Vandasue L.
Dolde, Ulla
Sun, Bin
Blaakmeer, Anko
Straub, Daniel
Eguen, Tenai
Botterweg-Paredes, Esther
Hong, Shinyoung
Graeff, Moritz
Li, Man-Wah
Gendron, Joshua M.
Wenkel, Stephan
author_sort Rodrigues, Vandasue L.
collection PubMed
description MicroProteins are potent post-translational regulators. In Arabidopsis (Arabidopsis thaliana), the miP1a/b microProteins delay floral transition by forming a complex with CONSTANS (CO) and the co-repressor protein TOPLESS. To better understand the function of the miP1a microProtein in floral repression, we performed a genetic suppressor screen to identify suppressors of miP1a (sum) function. One mutant, sum1, exhibited strong suppression of the miP1a-induced late-flowering phenotype. Mapping of sum1 identified another allele of the gene encoding the histone H3K4 demethylase JUMONJI14 (JMJ14), which is required for miP1a function. Plants carrying mutations in JMJ14 exhibit an early flowering phenotype that is largely dependent on CO activity, supporting an additional role for CO in the repressive complex. We further investigated whether miP1a function involves chromatin modification, performed whole-genome methylome sequencing studies with plants ectopically expressing miP1a, and identified differentially methylated regions (DMRs). Among these DMRs is the promoter of FLOWERING LOCUS T (FT), the prime target of miP1a that is ectopically methylated in a JMJ14-dependent manner. Moreover, when aberrantly expressed at the shoot apex, CO induces early flowering, but only when JMJ14 is mutated. Detailed analysis of the genetic interaction among CO, JMJ14, miP1a/b, and TPL revealed a potential role for CO as a repressor of flowering in the shoot apical meristem (SAM). Altogether, our results suggest that a repressor complex operates in the SAM, likely to maintain it in an undifferentiated state until leaf-derived florigen signals induce SAM conversion into a floral meristem.
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spelling pubmed-84184332021-09-09 A microProtein repressor complex in the shoot meristem controls the transition to flowering Rodrigues, Vandasue L. Dolde, Ulla Sun, Bin Blaakmeer, Anko Straub, Daniel Eguen, Tenai Botterweg-Paredes, Esther Hong, Shinyoung Graeff, Moritz Li, Man-Wah Gendron, Joshua M. Wenkel, Stephan Plant Physiol Research Articles MicroProteins are potent post-translational regulators. In Arabidopsis (Arabidopsis thaliana), the miP1a/b microProteins delay floral transition by forming a complex with CONSTANS (CO) and the co-repressor protein TOPLESS. To better understand the function of the miP1a microProtein in floral repression, we performed a genetic suppressor screen to identify suppressors of miP1a (sum) function. One mutant, sum1, exhibited strong suppression of the miP1a-induced late-flowering phenotype. Mapping of sum1 identified another allele of the gene encoding the histone H3K4 demethylase JUMONJI14 (JMJ14), which is required for miP1a function. Plants carrying mutations in JMJ14 exhibit an early flowering phenotype that is largely dependent on CO activity, supporting an additional role for CO in the repressive complex. We further investigated whether miP1a function involves chromatin modification, performed whole-genome methylome sequencing studies with plants ectopically expressing miP1a, and identified differentially methylated regions (DMRs). Among these DMRs is the promoter of FLOWERING LOCUS T (FT), the prime target of miP1a that is ectopically methylated in a JMJ14-dependent manner. Moreover, when aberrantly expressed at the shoot apex, CO induces early flowering, but only when JMJ14 is mutated. Detailed analysis of the genetic interaction among CO, JMJ14, miP1a/b, and TPL revealed a potential role for CO as a repressor of flowering in the shoot apical meristem (SAM). Altogether, our results suggest that a repressor complex operates in the SAM, likely to maintain it in an undifferentiated state until leaf-derived florigen signals induce SAM conversion into a floral meristem. Oxford University Press 2021-05-20 /pmc/articles/PMC8418433/ /pubmed/34015131 http://dx.doi.org/10.1093/plphys/kiab235 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Rodrigues, Vandasue L.
Dolde, Ulla
Sun, Bin
Blaakmeer, Anko
Straub, Daniel
Eguen, Tenai
Botterweg-Paredes, Esther
Hong, Shinyoung
Graeff, Moritz
Li, Man-Wah
Gendron, Joshua M.
Wenkel, Stephan
A microProtein repressor complex in the shoot meristem controls the transition to flowering
title A microProtein repressor complex in the shoot meristem controls the transition to flowering
title_full A microProtein repressor complex in the shoot meristem controls the transition to flowering
title_fullStr A microProtein repressor complex in the shoot meristem controls the transition to flowering
title_full_unstemmed A microProtein repressor complex in the shoot meristem controls the transition to flowering
title_short A microProtein repressor complex in the shoot meristem controls the transition to flowering
title_sort microprotein repressor complex in the shoot meristem controls the transition to flowering
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418433/
https://www.ncbi.nlm.nih.gov/pubmed/34015131
http://dx.doi.org/10.1093/plphys/kiab235
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