Cargando…

Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis

PURPOSE: Serine protease inhibitors (SERPINs) family has been discovered in many disorders with proteolysis mechanisms. Our study determined the SERPINBs protein expression via public‐based GEO databases and further validated by peri‐implant crevicular fluid (PICF) of peri‐implantitis patients and h...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Jianhui, Gao, Guanglin, Wang, Qingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418482/
https://www.ncbi.nlm.nih.gov/pubmed/34347896
http://dx.doi.org/10.1002/jcla.23926
_version_ 1783748578792964096
author Jiang, Jianhui
Gao, Guanglin
Wang, Qingmei
author_facet Jiang, Jianhui
Gao, Guanglin
Wang, Qingmei
author_sort Jiang, Jianhui
collection PubMed
description PURPOSE: Serine protease inhibitors (SERPINs) family has been discovered in many disorders with proteolysis mechanisms. Our study determined the SERPINBs protein expression via public‐based GEO databases and further validated by peri‐implant crevicular fluid (PICF) of peri‐implantitis patients and healthy recruiters. METHODS: This study is a retrospective analysis. A total of 123 participants of Fujian Medical University Fujian Stomatological Hospital, consisting of 58 cases of peri‐implantitis and 65 samples of healthy control were retrospectively analyzed by ELISA assays and explored the gene enrichment pathways and clinical significance of SERPINBs expression accompanied by two different cytokines (IL‐6 and TNF‐α). Moreover, the clinical significance of SERPINBs was evaluated in peri‐implantitis patients with PICF samples by the receiver operating curve (ROC) using the area under the curve (AUC). RESULTS: KEGG database showed that Starch and sucrose metabolism, Retrograde endocannabinoid signaling, Prion diseases, Pentose phosphate pathways, and Olfactory pathways are up‐regulated; GO database showed that synapse organization, synapse assembly, sequestering of triglyceride, sensory perception of smell, and regulation of synapse organization pathways are up‐regulated. SERPINBs were overexpressed in peri‐implant tissues and peri‐implantitis patients with PICF. SERPINBs was positively correlated to IL‐6 and TNF‐α in peri‐implantitis patients with PICF. The ROC‐AUCs of SERPINBs achieved a significantly higher range from 0.895 to 0.939 in peri‐implantitis patients with PICF. Therefore, certain SERPINBs expressions were not only perceived through PICF and peri‐implant tissues but also showed potential significance in peri‐implantitis. CONCLUSION: SERPINBs play an influential role in the pathogenesis of peri‐implantitis via binding with other inflammatory cytokines.
format Online
Article
Text
id pubmed-8418482
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-84184822021-09-08 Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis Jiang, Jianhui Gao, Guanglin Wang, Qingmei J Clin Lab Anal Research Articles PURPOSE: Serine protease inhibitors (SERPINs) family has been discovered in many disorders with proteolysis mechanisms. Our study determined the SERPINBs protein expression via public‐based GEO databases and further validated by peri‐implant crevicular fluid (PICF) of peri‐implantitis patients and healthy recruiters. METHODS: This study is a retrospective analysis. A total of 123 participants of Fujian Medical University Fujian Stomatological Hospital, consisting of 58 cases of peri‐implantitis and 65 samples of healthy control were retrospectively analyzed by ELISA assays and explored the gene enrichment pathways and clinical significance of SERPINBs expression accompanied by two different cytokines (IL‐6 and TNF‐α). Moreover, the clinical significance of SERPINBs was evaluated in peri‐implantitis patients with PICF samples by the receiver operating curve (ROC) using the area under the curve (AUC). RESULTS: KEGG database showed that Starch and sucrose metabolism, Retrograde endocannabinoid signaling, Prion diseases, Pentose phosphate pathways, and Olfactory pathways are up‐regulated; GO database showed that synapse organization, synapse assembly, sequestering of triglyceride, sensory perception of smell, and regulation of synapse organization pathways are up‐regulated. SERPINBs were overexpressed in peri‐implant tissues and peri‐implantitis patients with PICF. SERPINBs was positively correlated to IL‐6 and TNF‐α in peri‐implantitis patients with PICF. The ROC‐AUCs of SERPINBs achieved a significantly higher range from 0.895 to 0.939 in peri‐implantitis patients with PICF. Therefore, certain SERPINBs expressions were not only perceived through PICF and peri‐implant tissues but also showed potential significance in peri‐implantitis. CONCLUSION: SERPINBs play an influential role in the pathogenesis of peri‐implantitis via binding with other inflammatory cytokines. John Wiley and Sons Inc. 2021-08-04 /pmc/articles/PMC8418482/ /pubmed/34347896 http://dx.doi.org/10.1002/jcla.23926 Text en © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Jiang, Jianhui
Gao, Guanglin
Wang, Qingmei
Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title_full Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title_fullStr Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title_full_unstemmed Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title_short Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
title_sort levels of serpin family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418482/
https://www.ncbi.nlm.nih.gov/pubmed/34347896
http://dx.doi.org/10.1002/jcla.23926
work_keys_str_mv AT jiangjianhui levelsofserpinfamilyproteinsinperiimplantcrevicularfluidinpatientswithperiimplantitis
AT gaoguanglin levelsofserpinfamilyproteinsinperiimplantcrevicularfluidinpatientswithperiimplantitis
AT wangqingmei levelsofserpinfamilyproteinsinperiimplantcrevicularfluidinpatientswithperiimplantitis