Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity

BACKGROUND: It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test(®) CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of...

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Autores principales: Kon, Hadas, Abramov, Shirin, Frenk, Sammy, Schwartz, David, Shalom, Ohad, Adler, Amos, Carmeli, Yehuda, Lellouche, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418752/
https://www.ncbi.nlm.nih.gov/pubmed/34481497
http://dx.doi.org/10.1186/s12941-021-00469-0
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author Kon, Hadas
Abramov, Shirin
Frenk, Sammy
Schwartz, David
Shalom, Ohad
Adler, Amos
Carmeli, Yehuda
Lellouche, Jonathan
author_facet Kon, Hadas
Abramov, Shirin
Frenk, Sammy
Schwartz, David
Shalom, Ohad
Adler, Amos
Carmeli, Yehuda
Lellouche, Jonathan
author_sort Kon, Hadas
collection PubMed
description BACKGROUND: It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test(®) CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies. METHODS: A total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification. RESULTS: Excellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a bla(SME) positive S. marcescens isolate) was incorrectly positive for bla(OXA-48) by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively. CONCLUSIONS: The CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of the main carbapenemases of interest in clinical microbiology laboratories.
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spelling pubmed-84187522021-09-09 Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity Kon, Hadas Abramov, Shirin Frenk, Sammy Schwartz, David Shalom, Ohad Adler, Amos Carmeli, Yehuda Lellouche, Jonathan Ann Clin Microbiol Antimicrob Research BACKGROUND: It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test(®) CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies. METHODS: A total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification. RESULTS: Excellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a bla(SME) positive S. marcescens isolate) was incorrectly positive for bla(OXA-48) by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively. CONCLUSIONS: The CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of the main carbapenemases of interest in clinical microbiology laboratories. BioMed Central 2021-09-04 /pmc/articles/PMC8418752/ /pubmed/34481497 http://dx.doi.org/10.1186/s12941-021-00469-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kon, Hadas
Abramov, Shirin
Frenk, Sammy
Schwartz, David
Shalom, Ohad
Adler, Amos
Carmeli, Yehuda
Lellouche, Jonathan
Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title_full Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title_fullStr Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title_full_unstemmed Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title_short Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity
title_sort multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of oxa-48-like cross reactivity
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418752/
https://www.ncbi.nlm.nih.gov/pubmed/34481497
http://dx.doi.org/10.1186/s12941-021-00469-0
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