Cargando…

Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer

OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of se...

Descripción completa

Detalles Bibliográficos
Autores principales: Elnaggar, Ghada Nabil, El-Hifnawi, Niveen M, Ismail, Abeer, Yahia, Maha, Elshimy, Reham A A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418855/
https://www.ncbi.nlm.nih.gov/pubmed/34181356
http://dx.doi.org/10.31557/APJCP.2021.22.6.1949
_version_ 1783748646595985408
author Elnaggar, Ghada Nabil
El-Hifnawi, Niveen M
Ismail, Abeer
Yahia, Maha
Elshimy, Reham A A
author_facet Elnaggar, Ghada Nabil
El-Hifnawi, Niveen M
Ismail, Abeer
Yahia, Maha
Elshimy, Reham A A
author_sort Elnaggar, Ghada Nabil
collection PubMed
description OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of serum miR-148a as a non-invasive biomarker in non-small cell lung cancer (NSCLC) patients and to assess the correlation between miR-148a and Bcl-2, as one of its target proteins. MATERIALS AND METHODS: A total of 50 newly diagnosed NSCLC cases and 30 apparently healthy controls were recruited in this study. MiR-148a level was measured by TaqMan- Real time RT-PCR assay and Bcl-2 level was measured by ELISA. RESULTS: Significant lower expression of serum miR-148a and higher serum Bcl-2 levels were observed in NSCLC patients as compared to the control group (p <0.001 each). A statistically significant inverse correlation was also evident between miR-148a and Bcl-2. Lower miR-148a gene expression level and higher Bcl-2 concentration were found to be associated with advanced tumor stage, lymph node involvement and distant metastasis. CONCLUSION: MiR-148a could be a possible biomarker for NSCLC and by targeting Bcl-2, it may offer a novel approach for treatment.
format Online
Article
Text
id pubmed-8418855
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher West Asia Organization for Cancer Prevention
record_format MEDLINE/PubMed
spelling pubmed-84188552021-09-10 Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer Elnaggar, Ghada Nabil El-Hifnawi, Niveen M Ismail, Abeer Yahia, Maha Elshimy, Reham A A Asian Pac J Cancer Prev Research Article OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of serum miR-148a as a non-invasive biomarker in non-small cell lung cancer (NSCLC) patients and to assess the correlation between miR-148a and Bcl-2, as one of its target proteins. MATERIALS AND METHODS: A total of 50 newly diagnosed NSCLC cases and 30 apparently healthy controls were recruited in this study. MiR-148a level was measured by TaqMan- Real time RT-PCR assay and Bcl-2 level was measured by ELISA. RESULTS: Significant lower expression of serum miR-148a and higher serum Bcl-2 levels were observed in NSCLC patients as compared to the control group (p <0.001 each). A statistically significant inverse correlation was also evident between miR-148a and Bcl-2. Lower miR-148a gene expression level and higher Bcl-2 concentration were found to be associated with advanced tumor stage, lymph node involvement and distant metastasis. CONCLUSION: MiR-148a could be a possible biomarker for NSCLC and by targeting Bcl-2, it may offer a novel approach for treatment. West Asia Organization for Cancer Prevention 2021-06 /pmc/articles/PMC8418855/ /pubmed/34181356 http://dx.doi.org/10.31557/APJCP.2021.22.6.1949 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Elnaggar, Ghada Nabil
El-Hifnawi, Niveen M
Ismail, Abeer
Yahia, Maha
Elshimy, Reham A A
Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title_full Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title_fullStr Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title_full_unstemmed Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title_short Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
title_sort micro rna-148a targets bcl-2 in patients with non-small cell lung cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418855/
https://www.ncbi.nlm.nih.gov/pubmed/34181356
http://dx.doi.org/10.31557/APJCP.2021.22.6.1949
work_keys_str_mv AT elnaggarghadanabil microrna148atargetsbcl2inpatientswithnonsmallcelllungcancer
AT elhifnawiniveenm microrna148atargetsbcl2inpatientswithnonsmallcelllungcancer
AT ismailabeer microrna148atargetsbcl2inpatientswithnonsmallcelllungcancer
AT yahiamaha microrna148atargetsbcl2inpatientswithnonsmallcelllungcancer
AT elshimyrehamaa microrna148atargetsbcl2inpatientswithnonsmallcelllungcancer