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Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer
OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of se...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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West Asia Organization for Cancer Prevention
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418855/ https://www.ncbi.nlm.nih.gov/pubmed/34181356 http://dx.doi.org/10.31557/APJCP.2021.22.6.1949 |
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author | Elnaggar, Ghada Nabil El-Hifnawi, Niveen M Ismail, Abeer Yahia, Maha Elshimy, Reham A A |
author_facet | Elnaggar, Ghada Nabil El-Hifnawi, Niveen M Ismail, Abeer Yahia, Maha Elshimy, Reham A A |
author_sort | Elnaggar, Ghada Nabil |
collection | PubMed |
description | OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of serum miR-148a as a non-invasive biomarker in non-small cell lung cancer (NSCLC) patients and to assess the correlation between miR-148a and Bcl-2, as one of its target proteins. MATERIALS AND METHODS: A total of 50 newly diagnosed NSCLC cases and 30 apparently healthy controls were recruited in this study. MiR-148a level was measured by TaqMan- Real time RT-PCR assay and Bcl-2 level was measured by ELISA. RESULTS: Significant lower expression of serum miR-148a and higher serum Bcl-2 levels were observed in NSCLC patients as compared to the control group (p <0.001 each). A statistically significant inverse correlation was also evident between miR-148a and Bcl-2. Lower miR-148a gene expression level and higher Bcl-2 concentration were found to be associated with advanced tumor stage, lymph node involvement and distant metastasis. CONCLUSION: MiR-148a could be a possible biomarker for NSCLC and by targeting Bcl-2, it may offer a novel approach for treatment. |
format | Online Article Text |
id | pubmed-8418855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-84188552021-09-10 Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer Elnaggar, Ghada Nabil El-Hifnawi, Niveen M Ismail, Abeer Yahia, Maha Elshimy, Reham A A Asian Pac J Cancer Prev Research Article OBJECTIVE: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide. MicroRNAs regulate more than 60% of human genes, including tumor suppressor genes and oncogenes. Accordingly, they can affect cancer risk. This study aimed to evaluate the role of serum miR-148a as a non-invasive biomarker in non-small cell lung cancer (NSCLC) patients and to assess the correlation between miR-148a and Bcl-2, as one of its target proteins. MATERIALS AND METHODS: A total of 50 newly diagnosed NSCLC cases and 30 apparently healthy controls were recruited in this study. MiR-148a level was measured by TaqMan- Real time RT-PCR assay and Bcl-2 level was measured by ELISA. RESULTS: Significant lower expression of serum miR-148a and higher serum Bcl-2 levels were observed in NSCLC patients as compared to the control group (p <0.001 each). A statistically significant inverse correlation was also evident between miR-148a and Bcl-2. Lower miR-148a gene expression level and higher Bcl-2 concentration were found to be associated with advanced tumor stage, lymph node involvement and distant metastasis. CONCLUSION: MiR-148a could be a possible biomarker for NSCLC and by targeting Bcl-2, it may offer a novel approach for treatment. West Asia Organization for Cancer Prevention 2021-06 /pmc/articles/PMC8418855/ /pubmed/34181356 http://dx.doi.org/10.31557/APJCP.2021.22.6.1949 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Elnaggar, Ghada Nabil El-Hifnawi, Niveen M Ismail, Abeer Yahia, Maha Elshimy, Reham A A Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title | Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title_full | Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title_fullStr | Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title_full_unstemmed | Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title_short | Micro RNA-148a Targets Bcl-2 in Patients with Non-Small Cell Lung Cancer |
title_sort | micro rna-148a targets bcl-2 in patients with non-small cell lung cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418855/ https://www.ncbi.nlm.nih.gov/pubmed/34181356 http://dx.doi.org/10.31557/APJCP.2021.22.6.1949 |
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