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Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq

Understanding of the complex interaction between the peripheral immune system and lung cancer (LC) remains incomplete, limiting patient benefit. Here, we aimed to characterize the host peripheral immune response to LC and investigate its potential prognostic value. Bulk RNA‐sequencing data of periph...

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Autores principales: Zhang, Qi, Kuang, Manchao, An, Haiyin, Zhang, Yajing, Zhang, Kai, Feng, Lin, Zhang, Lei, Cheng, Shujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419186/
https://www.ncbi.nlm.nih.gov/pubmed/34288383
http://dx.doi.org/10.1111/jcmm.16773
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author Zhang, Qi
Kuang, Manchao
An, Haiyin
Zhang, Yajing
Zhang, Kai
Feng, Lin
Zhang, Lei
Cheng, Shujun
author_facet Zhang, Qi
Kuang, Manchao
An, Haiyin
Zhang, Yajing
Zhang, Kai
Feng, Lin
Zhang, Lei
Cheng, Shujun
author_sort Zhang, Qi
collection PubMed
description Understanding of the complex interaction between the peripheral immune system and lung cancer (LC) remains incomplete, limiting patient benefit. Here, we aimed to characterize the host peripheral immune response to LC and investigate its potential prognostic value. Bulk RNA‐sequencing data of peripheral blood leucocytes (PBLs) from healthy volunteers and LC patients (n = 142) were analysed for characterization of host systemic immunity in LC. We observed broad blood transcriptome perturbations in LC patients that were heterogeneous, as two new subtypes were established independent of histology. Functionally, the heterogeneity between the two subtypes included dysregulation of diverse biological processes, such as the cell cycle, blood coagulation and inflammatory signalling pathways, together with the abundance and activity of blood cells, particularly lymphocytes and neutrophils, ultimately manifesting as differences in antitumour immune status. Based on these findings, a prognostic model composed of ten genes dysregulated in one LC subtype with relatively poor immune status was developed and validated in a Gene Expression Omnibus (GEO) data set (n = 108), helping to generate a prognostic nomogram. Collectively, our study provides novel and comprehensive insight into the heterogeneity of the host peripheral immune response to LC. The expression heterogeneity–based predictive model may help guide prognostic management for LC patients.
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spelling pubmed-84191862021-09-08 Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq Zhang, Qi Kuang, Manchao An, Haiyin Zhang, Yajing Zhang, Kai Feng, Lin Zhang, Lei Cheng, Shujun J Cell Mol Med Original Articles Understanding of the complex interaction between the peripheral immune system and lung cancer (LC) remains incomplete, limiting patient benefit. Here, we aimed to characterize the host peripheral immune response to LC and investigate its potential prognostic value. Bulk RNA‐sequencing data of peripheral blood leucocytes (PBLs) from healthy volunteers and LC patients (n = 142) were analysed for characterization of host systemic immunity in LC. We observed broad blood transcriptome perturbations in LC patients that were heterogeneous, as two new subtypes were established independent of histology. Functionally, the heterogeneity between the two subtypes included dysregulation of diverse biological processes, such as the cell cycle, blood coagulation and inflammatory signalling pathways, together with the abundance and activity of blood cells, particularly lymphocytes and neutrophils, ultimately manifesting as differences in antitumour immune status. Based on these findings, a prognostic model composed of ten genes dysregulated in one LC subtype with relatively poor immune status was developed and validated in a Gene Expression Omnibus (GEO) data set (n = 108), helping to generate a prognostic nomogram. Collectively, our study provides novel and comprehensive insight into the heterogeneity of the host peripheral immune response to LC. The expression heterogeneity–based predictive model may help guide prognostic management for LC patients. John Wiley and Sons Inc. 2021-07-21 2021-09 /pmc/articles/PMC8419186/ /pubmed/34288383 http://dx.doi.org/10.1111/jcmm.16773 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine and Foundation for Cellular and Molecular Medicine published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhang, Qi
Kuang, Manchao
An, Haiyin
Zhang, Yajing
Zhang, Kai
Feng, Lin
Zhang, Lei
Cheng, Shujun
Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title_full Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title_fullStr Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title_full_unstemmed Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title_short Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
title_sort peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by rna‐seq
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419186/
https://www.ncbi.nlm.nih.gov/pubmed/34288383
http://dx.doi.org/10.1111/jcmm.16773
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