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3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell type...

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Autores principales: Royan, Muhammad Rahmad, Siddique, Khadeeja, Csucs, Gergely, Puchades, Maja A., Nourizadeh-Lillabadi, Rasoul, Bjaalie, Jan G., Henkel, Christiaan V., Weltzien, Finn-Arne, Fontaine, Romain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419251/
https://www.ncbi.nlm.nih.gov/pubmed/34497587
http://dx.doi.org/10.3389/fendo.2021.719843
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author Royan, Muhammad Rahmad
Siddique, Khadeeja
Csucs, Gergely
Puchades, Maja A.
Nourizadeh-Lillabadi, Rasoul
Bjaalie, Jan G.
Henkel, Christiaan V.
Weltzien, Finn-Arne
Fontaine, Romain
author_facet Royan, Muhammad Rahmad
Siddique, Khadeeja
Csucs, Gergely
Puchades, Maja A.
Nourizadeh-Lillabadi, Rasoul
Bjaalie, Jan G.
Henkel, Christiaan V.
Weltzien, Finn-Arne
Fontaine, Romain
author_sort Royan, Muhammad Rahmad
collection PubMed
description In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell types is required to better understand their physiological functions. Using medaka as a model and several combinations of multi-color fluorescence in situ hybridization, we present the first 3D atlas revealing the gland-wide distribution of seven endocrine cell populations: lactotropes, thyrotropes, Lh and Fsh gonadotropes, somatotropes, and pomca-expressing cells (corticotropes and melanotropes) in the anterior pituitary of a teleost fish. By combining in situ hybridization and immunofluorescence techniques, we deciphered the location of corticotropes and melanotropes within the pomca-expressing cell population. The 3D localization approach reveals sexual dimorphism of tshba-, pomca-, and lhb-expressing cells in the adult medaka pituitary. Finally, we show the existence of bi-hormonal cells co-expressing lhb-fshb, fshb-tshba and lhb-sl using single-cell transcriptomics analysis and in situ hybridization. This study offers a solid basis for future comparative studies of the teleost pituitary and its functional plasticity.
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spelling pubmed-84192512021-09-07 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes) Royan, Muhammad Rahmad Siddique, Khadeeja Csucs, Gergely Puchades, Maja A. Nourizadeh-Lillabadi, Rasoul Bjaalie, Jan G. Henkel, Christiaan V. Weltzien, Finn-Arne Fontaine, Romain Front Endocrinol (Lausanne) Endocrinology In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell types is required to better understand their physiological functions. Using medaka as a model and several combinations of multi-color fluorescence in situ hybridization, we present the first 3D atlas revealing the gland-wide distribution of seven endocrine cell populations: lactotropes, thyrotropes, Lh and Fsh gonadotropes, somatotropes, and pomca-expressing cells (corticotropes and melanotropes) in the anterior pituitary of a teleost fish. By combining in situ hybridization and immunofluorescence techniques, we deciphered the location of corticotropes and melanotropes within the pomca-expressing cell population. The 3D localization approach reveals sexual dimorphism of tshba-, pomca-, and lhb-expressing cells in the adult medaka pituitary. Finally, we show the existence of bi-hormonal cells co-expressing lhb-fshb, fshb-tshba and lhb-sl using single-cell transcriptomics analysis and in situ hybridization. This study offers a solid basis for future comparative studies of the teleost pituitary and its functional plasticity. Frontiers Media S.A. 2021-08-23 /pmc/articles/PMC8419251/ /pubmed/34497587 http://dx.doi.org/10.3389/fendo.2021.719843 Text en Copyright © 2021 Royan, Siddique, Csucs, Puchades, Nourizadeh-Lillabadi, Bjaalie, Henkel, Weltzien and Fontaine https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Royan, Muhammad Rahmad
Siddique, Khadeeja
Csucs, Gergely
Puchades, Maja A.
Nourizadeh-Lillabadi, Rasoul
Bjaalie, Jan G.
Henkel, Christiaan V.
Weltzien, Finn-Arne
Fontaine, Romain
3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title_full 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title_fullStr 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title_full_unstemmed 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title_short 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)
title_sort 3d atlas of the pituitary gland of the model fish medaka (oryzias latipes)
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419251/
https://www.ncbi.nlm.nih.gov/pubmed/34497587
http://dx.doi.org/10.3389/fendo.2021.719843
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