Cargando…
Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity
PURPOSE: A novel coronavirus (COVID-19) that has not been previously identified in humans and has no specific treatment has recently spread. Treatment trials using antiviral and immune-modulating drugs such as hydroxychloroquine (HCQ) were used to control this viral outbreak however several side eff...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419391/ https://www.ncbi.nlm.nih.gov/pubmed/34513113 http://dx.doi.org/10.1007/s40005-021-00544-w |
_version_ | 1783748741458558976 |
---|---|
author | Ghareeb, Doaa A. Saleh, Samar R. Seadawy, Mohamed G. Nofal, Mohammed S. Abdulmalek, Shaymaa A. Hassan, Salma F. Khedr, Shaimaa M. AbdElwahab, Miral G. Sobhy, Ahmed A. Abdel-Hamid, Ali saber Ali Yassin, Abdelrahman Mohamed Elmoneam, Alshimaa A. Abd Masoud, Aliaa A. Kaddah, Mohamed M. Y. El-Zahaby, Sally A. Al-mahallawi, Abdulaziz Mohsen El-Gharbawy, Alaa M. Zaki, Ahmed Seif, Inas k. Kenawy, Marwa Y. Amin, Magdy Amer, Khaled El Demellawy, Maha Adel |
author_facet | Ghareeb, Doaa A. Saleh, Samar R. Seadawy, Mohamed G. Nofal, Mohammed S. Abdulmalek, Shaymaa A. Hassan, Salma F. Khedr, Shaimaa M. AbdElwahab, Miral G. Sobhy, Ahmed A. Abdel-Hamid, Ali saber Ali Yassin, Abdelrahman Mohamed Elmoneam, Alshimaa A. Abd Masoud, Aliaa A. Kaddah, Mohamed M. Y. El-Zahaby, Sally A. Al-mahallawi, Abdulaziz Mohsen El-Gharbawy, Alaa M. Zaki, Ahmed Seif, Inas k. Kenawy, Marwa Y. Amin, Magdy Amer, Khaled El Demellawy, Maha Adel |
author_sort | Ghareeb, Doaa A. |
collection | PubMed |
description | PURPOSE: A novel coronavirus (COVID-19) that has not been previously identified in humans and has no specific treatment has recently spread. Treatment trials using antiviral and immune-modulating drugs such as hydroxychloroquine (HCQ) were used to control this viral outbreak however several side effects have emerged. Berberine (BER) is an alkaloid that has been reported to reveal some pharmacological properties including antioxidant and antimicrobial activities. Additionally, Zinc oxide nanoparticles (ZnO-NPs) possess potent antioxidant and anti-inflammatory properties. Therefore, this study was undertaken to estimate the efficiency of both BER and synthetic ZnO/BER complex as an anti-COVID-19 therapy. METHODS: First, the ZnO/BER complex was prepared by the facile mixing method. Then in vitro studies on the two compounds were conducted including VeroE6 toxicity, anti-COVID-19 activity, determination of inhibitory activity towards papain-like proteinase (PL pro) and spike protein- and receptor- binding domain (RBD) as well as assessment of drug toxicity on RBCs. RESULTS: The results showed that ZnO/BER complex acts as an anti-COVID-19 by inhibiting spike protein binding with angiotensin-converting enzyme II (ACE II), PL pro activity, spike protein and E protein levels, and expression of both E-gene and RNA dependent RNA polymerase (RdRp) at a concentration lower than that of BER or ZnO-NPs alone. Furthermore, ZnO/BER complex had antioxidant and antimicrobial properties where it prevents the auto oxidation of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and the culture of lower respiratory system bacteria that affected Covid 19 patients. The ZnO/BER complex prevented as well the HCQ cytotoxic effect on both RBC and WBC (in vitro) and hepatotoxicity, nephrotoxicity and anemia that occurred after HCQ long administration in vivo. CONCLUSION: The ZnO/BER complex can be accounted as promising anti-COVID 19 candidate because it inhibited the virus entry, replication, and assembly. Furthermore, it could be used to treat a second bacterial infection that took place in hospitalized COVID 19 patients. Moreover, ZnO/BER complex was found to eliminate the toxicity of long-term administration of HCQ in vivo. |
format | Online Article Text |
id | pubmed-8419391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-84193912021-09-07 Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity Ghareeb, Doaa A. Saleh, Samar R. Seadawy, Mohamed G. Nofal, Mohammed S. Abdulmalek, Shaymaa A. Hassan, Salma F. Khedr, Shaimaa M. AbdElwahab, Miral G. Sobhy, Ahmed A. Abdel-Hamid, Ali saber Ali Yassin, Abdelrahman Mohamed Elmoneam, Alshimaa A. Abd Masoud, Aliaa A. Kaddah, Mohamed M. Y. El-Zahaby, Sally A. Al-mahallawi, Abdulaziz Mohsen El-Gharbawy, Alaa M. Zaki, Ahmed Seif, Inas k. Kenawy, Marwa Y. Amin, Magdy Amer, Khaled El Demellawy, Maha Adel J Pharm Investig Original Article PURPOSE: A novel coronavirus (COVID-19) that has not been previously identified in humans and has no specific treatment has recently spread. Treatment trials using antiviral and immune-modulating drugs such as hydroxychloroquine (HCQ) were used to control this viral outbreak however several side effects have emerged. Berberine (BER) is an alkaloid that has been reported to reveal some pharmacological properties including antioxidant and antimicrobial activities. Additionally, Zinc oxide nanoparticles (ZnO-NPs) possess potent antioxidant and anti-inflammatory properties. Therefore, this study was undertaken to estimate the efficiency of both BER and synthetic ZnO/BER complex as an anti-COVID-19 therapy. METHODS: First, the ZnO/BER complex was prepared by the facile mixing method. Then in vitro studies on the two compounds were conducted including VeroE6 toxicity, anti-COVID-19 activity, determination of inhibitory activity towards papain-like proteinase (PL pro) and spike protein- and receptor- binding domain (RBD) as well as assessment of drug toxicity on RBCs. RESULTS: The results showed that ZnO/BER complex acts as an anti-COVID-19 by inhibiting spike protein binding with angiotensin-converting enzyme II (ACE II), PL pro activity, spike protein and E protein levels, and expression of both E-gene and RNA dependent RNA polymerase (RdRp) at a concentration lower than that of BER or ZnO-NPs alone. Furthermore, ZnO/BER complex had antioxidant and antimicrobial properties where it prevents the auto oxidation of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and the culture of lower respiratory system bacteria that affected Covid 19 patients. The ZnO/BER complex prevented as well the HCQ cytotoxic effect on both RBC and WBC (in vitro) and hepatotoxicity, nephrotoxicity and anemia that occurred after HCQ long administration in vivo. CONCLUSION: The ZnO/BER complex can be accounted as promising anti-COVID 19 candidate because it inhibited the virus entry, replication, and assembly. Furthermore, it could be used to treat a second bacterial infection that took place in hospitalized COVID 19 patients. Moreover, ZnO/BER complex was found to eliminate the toxicity of long-term administration of HCQ in vivo. Springer Singapore 2021-09-06 2021 /pmc/articles/PMC8419391/ /pubmed/34513113 http://dx.doi.org/10.1007/s40005-021-00544-w Text en © The Korean Society of Pharmaceutical Sciences and Technology 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Ghareeb, Doaa A. Saleh, Samar R. Seadawy, Mohamed G. Nofal, Mohammed S. Abdulmalek, Shaymaa A. Hassan, Salma F. Khedr, Shaimaa M. AbdElwahab, Miral G. Sobhy, Ahmed A. Abdel-Hamid, Ali saber Ali Yassin, Abdelrahman Mohamed Elmoneam, Alshimaa A. Abd Masoud, Aliaa A. Kaddah, Mohamed M. Y. El-Zahaby, Sally A. Al-mahallawi, Abdulaziz Mohsen El-Gharbawy, Alaa M. Zaki, Ahmed Seif, Inas k. Kenawy, Marwa Y. Amin, Magdy Amer, Khaled El Demellawy, Maha Adel Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title | Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title_full | Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title_fullStr | Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title_full_unstemmed | Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title_short | Nanoparticles of ZnO/Berberine complex contract COVID-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
title_sort | nanoparticles of zno/berberine complex contract covid-19 and respiratory co-bacterial infection in addition to elimination of hydroxychloroquine toxicity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419391/ https://www.ncbi.nlm.nih.gov/pubmed/34513113 http://dx.doi.org/10.1007/s40005-021-00544-w |
work_keys_str_mv | AT ghareebdoaaa nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT salehsamarr nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT seadawymohamedg nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT nofalmohammeds nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT abdulmalekshaymaaa nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT hassansalmaf nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT khedrshaimaam nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT abdelwahabmiralg nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT sobhyahmeda nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT abdelhamidalisaberali nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT yassinabdelrahmanmohamed nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT elmoneamalshimaaaabd nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT masoudaliaaa nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT kaddahmohamedmy nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT elzahabysallya nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT almahallawiabdulazizmohsen nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT elgharbawyalaam nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT zakiahmed nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT seifinask nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT kenawymarway nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT aminmagdy nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT amerkhaled nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity AT eldemellawymahaadel nanoparticlesofznoberberinecomplexcontractcovid19andrespiratorycobacterialinfectioninadditiontoeliminationofhydroxychloroquinetoxicity |