Cargando…
Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC)
BACKGROUND: Gastric cancer (GC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis combined with surgical treatment can significantly improve the prognosis of patients. Therefore, it is urgent to seek higher sensitivity and specificity biomarkers in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419412/ https://www.ncbi.nlm.nih.gov/pubmed/34497770 http://dx.doi.org/10.3389/fonc.2021.723753 |
_version_ | 1783748746502209536 |
---|---|
author | Huang, Yuejiao Zhang, Haiyan Gu, Xinliang Qin, Shiyi Zheng, Ming Shi, Xiangrong Peng, Chunlei Ju, Shaoqing |
author_facet | Huang, Yuejiao Zhang, Haiyan Gu, Xinliang Qin, Shiyi Zheng, Ming Shi, Xiangrong Peng, Chunlei Ju, Shaoqing |
author_sort | Huang, Yuejiao |
collection | PubMed |
description | BACKGROUND: Gastric cancer (GC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis combined with surgical treatment can significantly improve the prognosis of patients. Therefore, it is urgent to seek higher sensitivity and specificity biomarkers in GC. tRNA-derived small RNAs are a new non-coding small RNA that widely exists in tumor cells and body fluids. In this study, we explore the expression and biological significance of tRNA-derived small RNAs in GC. MATERIALS AND METHODS: First of all, we screened the differentially expressed tRNA-derived small RNAs in tumor tissues by high-throughput sequencing. Agarose gel electrophoresis (AGE), Sanger sequencing, and Nuclear and Cytoplasmic RNA Separation Assay were used to screen tRF-31-U5YKFN8DYDZDD as a potential tumor biomarker for the diagnosis of GC. Then, we detected the different expressions of tRF-31-U5YKFN8DYDZDD in 24 pairs of GC and paracancerous tissues, the serum of 111 GC patients at first diagnosis, 89 normal subjects, 48 superficial gastritis patients, and 28 postoperative GC patients by quantitative real-time PCR (qRT-PCR). Finally, we used the receiver operating characteristic (ROC) curve to analyze its diagnostic efficacy. RESULTS: The expression of tRF-31-U5YKFN8DYDZDD has good stability and easy detection. tRF-31-U5YKFN8DYDZDD was highly expressed in tumor tissue, serum, and cell lines of GC, and the expression was significantly related to TNM stage, depth of tumor invasion, lymph node metastasis, and vascular invasion. The expression of serum tRF-31-U5YKFN8DYDZDD in the GC patients decreased after the operation (P = 0.0003). Combined with ROC curve analysis, tRF-31-U5YKFN8DYDZDD has better detection efficiency than conventional markers. CONCLUSIONS: The expressions of tRF-31-U5YKFN8DYDZDD in the tumor and paracancerous tissues, the serum of GC patients and healthy people, and the serum of GC patients before and after operation were different. tRF-31-U5YKFN8DYDZDD is not only a diagnostic biomarker of GC but also a predictor of poor prognosis. |
format | Online Article Text |
id | pubmed-8419412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84194122021-09-07 Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) Huang, Yuejiao Zhang, Haiyan Gu, Xinliang Qin, Shiyi Zheng, Ming Shi, Xiangrong Peng, Chunlei Ju, Shaoqing Front Oncol Oncology BACKGROUND: Gastric cancer (GC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis combined with surgical treatment can significantly improve the prognosis of patients. Therefore, it is urgent to seek higher sensitivity and specificity biomarkers in GC. tRNA-derived small RNAs are a new non-coding small RNA that widely exists in tumor cells and body fluids. In this study, we explore the expression and biological significance of tRNA-derived small RNAs in GC. MATERIALS AND METHODS: First of all, we screened the differentially expressed tRNA-derived small RNAs in tumor tissues by high-throughput sequencing. Agarose gel electrophoresis (AGE), Sanger sequencing, and Nuclear and Cytoplasmic RNA Separation Assay were used to screen tRF-31-U5YKFN8DYDZDD as a potential tumor biomarker for the diagnosis of GC. Then, we detected the different expressions of tRF-31-U5YKFN8DYDZDD in 24 pairs of GC and paracancerous tissues, the serum of 111 GC patients at first diagnosis, 89 normal subjects, 48 superficial gastritis patients, and 28 postoperative GC patients by quantitative real-time PCR (qRT-PCR). Finally, we used the receiver operating characteristic (ROC) curve to analyze its diagnostic efficacy. RESULTS: The expression of tRF-31-U5YKFN8DYDZDD has good stability and easy detection. tRF-31-U5YKFN8DYDZDD was highly expressed in tumor tissue, serum, and cell lines of GC, and the expression was significantly related to TNM stage, depth of tumor invasion, lymph node metastasis, and vascular invasion. The expression of serum tRF-31-U5YKFN8DYDZDD in the GC patients decreased after the operation (P = 0.0003). Combined with ROC curve analysis, tRF-31-U5YKFN8DYDZDD has better detection efficiency than conventional markers. CONCLUSIONS: The expressions of tRF-31-U5YKFN8DYDZDD in the tumor and paracancerous tissues, the serum of GC patients and healthy people, and the serum of GC patients before and after operation were different. tRF-31-U5YKFN8DYDZDD is not only a diagnostic biomarker of GC but also a predictor of poor prognosis. Frontiers Media S.A. 2021-08-23 /pmc/articles/PMC8419412/ /pubmed/34497770 http://dx.doi.org/10.3389/fonc.2021.723753 Text en Copyright © 2021 Huang, Zhang, Gu, Qin, Zheng, Shi, Peng and Ju https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Huang, Yuejiao Zhang, Haiyan Gu, Xinliang Qin, Shiyi Zheng, Ming Shi, Xiangrong Peng, Chunlei Ju, Shaoqing Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title | Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title_full | Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title_fullStr | Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title_full_unstemmed | Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title_short | Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC) |
title_sort | elucidating the role of serum trf-31-u5ykfn8dydzdd as a novel diagnostic biomarker in gastric cancer (gc) |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419412/ https://www.ncbi.nlm.nih.gov/pubmed/34497770 http://dx.doi.org/10.3389/fonc.2021.723753 |
work_keys_str_mv | AT huangyuejiao elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT zhanghaiyan elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT guxinliang elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT qinshiyi elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT zhengming elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT shixiangrong elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT pengchunlei elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc AT jushaoqing elucidatingtheroleofserumtrf31u5ykfn8dydzddasanoveldiagnosticbiomarkeringastriccancergc |