Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

Hydrogen sulfide (H(2)S) plays an important role in mammals as a signaling molecule. Recently, abnormal H(2)S concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer's disease, and Parkinson's disease. As regulating H(2)S concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to H(2)S research. To understand the role of H(2)S in pathophysiology and develop H(2)S-based therapies, it is necessary to measure the exact concentration of H(2)S within biological systems. But, H(2)S is volatile and can be easily oxidized. Besides, the active sites for several biological effects of H(2)S are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of H(2)S within live cells. This review provides a summary of recent developments in H(2)S detection methods for live cell analysis.