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Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations
Our understanding of the aetiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of ‘lesions’ generated using luciferase-expressing donor mice....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419713/ https://www.ncbi.nlm.nih.gov/pubmed/34382636 http://dx.doi.org/10.1242/dmm.049070 |
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author | Dorning, Ashley Dhami, Priya Panir, Kavita Hogg, Chloe Park, Emma Ferguson, Gregory D. Hargrove, Diane Karras, James Horne, Andrew W. Greaves, Erin |
author_facet | Dorning, Ashley Dhami, Priya Panir, Kavita Hogg, Chloe Park, Emma Ferguson, Gregory D. Hargrove, Diane Karras, James Horne, Andrew W. Greaves, Erin |
author_sort | Dorning, Ashley |
collection | PubMed |
description | Our understanding of the aetiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of ‘lesions’ generated using luciferase-expressing donor mice. We compared longitudinal bioluminescence and histology of lesions, sensory behaviour of mice with induced endometriosis and the impact of the gonadotropin-releasing hormone antagonist Cetrorelix on lesion regression and sensory behaviour. Four models of endometriosis were tested. We found that the nature of the donor uterine material was a key determinant of how chronic the lesions were, as well as their cellular composition. The severity of pain-like behaviour also varied across models. Although Cetrorelix significantly reduced lesion bioluminescence in all models, it had varying impacts on pain-like behaviour. Collectively, our results demonstrate key differences in the progression of the ‘disease’ across different mouse models of endometriosis. We propose that validation and testing in multiple models, each of which may be representative of the different subtypes/heterogeneity observed in women, should become a standard approach to discovery science in the field of endometriosis. |
format | Online Article Text |
id | pubmed-8419713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-84197132021-09-07 Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations Dorning, Ashley Dhami, Priya Panir, Kavita Hogg, Chloe Park, Emma Ferguson, Gregory D. Hargrove, Diane Karras, James Horne, Andrew W. Greaves, Erin Dis Model Mech Research Article Our understanding of the aetiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of ‘lesions’ generated using luciferase-expressing donor mice. We compared longitudinal bioluminescence and histology of lesions, sensory behaviour of mice with induced endometriosis and the impact of the gonadotropin-releasing hormone antagonist Cetrorelix on lesion regression and sensory behaviour. Four models of endometriosis were tested. We found that the nature of the donor uterine material was a key determinant of how chronic the lesions were, as well as their cellular composition. The severity of pain-like behaviour also varied across models. Although Cetrorelix significantly reduced lesion bioluminescence in all models, it had varying impacts on pain-like behaviour. Collectively, our results demonstrate key differences in the progression of the ‘disease’ across different mouse models of endometriosis. We propose that validation and testing in multiple models, each of which may be representative of the different subtypes/heterogeneity observed in women, should become a standard approach to discovery science in the field of endometriosis. The Company of Biologists Ltd 2021-08-31 /pmc/articles/PMC8419713/ /pubmed/34382636 http://dx.doi.org/10.1242/dmm.049070 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Dorning, Ashley Dhami, Priya Panir, Kavita Hogg, Chloe Park, Emma Ferguson, Gregory D. Hargrove, Diane Karras, James Horne, Andrew W. Greaves, Erin Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title | Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title_full | Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title_fullStr | Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title_full_unstemmed | Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title_short | Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
title_sort | bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419713/ https://www.ncbi.nlm.nih.gov/pubmed/34382636 http://dx.doi.org/10.1242/dmm.049070 |
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