Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2

The aim of the study was to evaluate the clinical performance of FTD SARS-CoV-2 compared to the RealStar RT-PCR kit 1.0. The analysis of 100 nasopharyngeal swabs showed an overall agreement of 88 %. The positive percentage agreement was 85.6 % and the negative percentage agreement was 91 %. In concl...

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Autores principales: Di Carlo, Daniele, Mazzuti, Laura, Sciandra, Ilaria, Guerrizio, Giuliana, Oliveto, Giuseppe, Riveros Cabral, Rodolfo J., Zingaropoli, Maria Antonella, Turriziani, Ombretta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420089/
https://www.ncbi.nlm.nih.gov/pubmed/34499965
http://dx.doi.org/10.1016/j.jviromet.2021.114276
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author Di Carlo, Daniele
Mazzuti, Laura
Sciandra, Ilaria
Guerrizio, Giuliana
Oliveto, Giuseppe
Riveros Cabral, Rodolfo J.
Zingaropoli, Maria Antonella
Turriziani, Ombretta
author_facet Di Carlo, Daniele
Mazzuti, Laura
Sciandra, Ilaria
Guerrizio, Giuliana
Oliveto, Giuseppe
Riveros Cabral, Rodolfo J.
Zingaropoli, Maria Antonella
Turriziani, Ombretta
author_sort Di Carlo, Daniele
collection PubMed
description The aim of the study was to evaluate the clinical performance of FTD SARS-CoV-2 compared to the RealStar RT-PCR kit 1.0. The analysis of 100 nasopharyngeal swabs showed an overall agreement of 88 %. The positive percentage agreement was 85.6 % and the negative percentage agreement was 91 %. In conclusion we observed a substantial agreement among the two methods, with discrepancies mainly observed in specimens with relatively low amount of viral RNA.
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spelling pubmed-84200892021-09-07 Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2 Di Carlo, Daniele Mazzuti, Laura Sciandra, Ilaria Guerrizio, Giuliana Oliveto, Giuseppe Riveros Cabral, Rodolfo J. Zingaropoli, Maria Antonella Turriziani, Ombretta J Virol Methods Short Communication The aim of the study was to evaluate the clinical performance of FTD SARS-CoV-2 compared to the RealStar RT-PCR kit 1.0. The analysis of 100 nasopharyngeal swabs showed an overall agreement of 88 %. The positive percentage agreement was 85.6 % and the negative percentage agreement was 91 %. In conclusion we observed a substantial agreement among the two methods, with discrepancies mainly observed in specimens with relatively low amount of viral RNA. Elsevier B.V. 2021-12 2021-09-06 /pmc/articles/PMC8420089/ /pubmed/34499965 http://dx.doi.org/10.1016/j.jviromet.2021.114276 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Di Carlo, Daniele
Mazzuti, Laura
Sciandra, Ilaria
Guerrizio, Giuliana
Oliveto, Giuseppe
Riveros Cabral, Rodolfo J.
Zingaropoli, Maria Antonella
Turriziani, Ombretta
Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title_full Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title_fullStr Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title_full_unstemmed Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title_short Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2
title_sort comparison of ftd sars-cov-2 assay and realstar rt-pcr kit 1.0 for the detection of sars-cov-2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420089/
https://www.ncbi.nlm.nih.gov/pubmed/34499965
http://dx.doi.org/10.1016/j.jviromet.2021.114276
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