Cargando…

Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies

Monoclonal anti‐SARS‐CoV‐2 immunoglobulins represent a treatment option for COVID‐19. However, their production in mammalian cells is not scalable to meet the global demand. Single‐domain (VHH) antibodies (also called nanobodies) provide an alternative suitable for microbial production. Using alpaca...

Descripción completa

Detalles Bibliográficos
Autores principales:	Güttler, Thomas, Aksu, Metin, Dickmanns, Antje, Stegmann, Kim M., Gregor, Kathrin, Rees, Renate, Taxer, Waltraud, Rymarenko, Oleh, Schünemann, Jürgen, Dienemann, Christian, Gunkel, Philip, Mussil, Bianka, Krull, Jens, Teichmann, Ulrike, Groß, Uwe, Cordes, Volker C, Dobbelstein, Matthias, Görlich, Dirk
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420576/ https://www.ncbi.nlm.nih.gov/pubmed/34302370 http://dx.doi.org/10.15252/embj.2021107985

Ejemplares similares

A nanobody toolbox to investigate localisation and dynamics of Drosophila titins and other key sarcomeric proteins
por: Loreau, Vincent, et al.
Publicado: (2023)

Therapeutische Nanobodies gegen SARS-CoV-2
por: Güttler, Thomas, et al.
Publicado: (2022)

N4-hydroxycytidine, the active compound of Molnupiravir, promotes SARS-CoV-2 mutagenesis and escape from a neutralizing nanobody
por: Zibat, Arne, et al.
Publicado: (2023)

Hyperthermostable acetyl xylan esterase
por: Drzewiecki, Katharina, et al.
Publicado: (2010)

Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles
por: Schueder, Florian, et al.
Publicado: (2023)

Creation of a functional hyperthermostable designer cellulosome
por: Kahn, Amaranta, et al.
Publicado: (2019)

A Novel Hyperthermostable Recombinant Protein Nanocage
por: Ahmadyousefi, Yaghoub, et al.
Publicado: (2022)

Crystal structures of FNIP/FGxxFN motif-containing leucine-rich repeat proteins
por: Huyton, Trevor, et al.
Publicado: (2022)

Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications
por: Rémy, Benjamin, et al.
Publicado: (2016)

ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR
por: Gunkel, Philip, et al.
Publicado: (2021)

An evolutionarily conserved bimodular domain anchors ZC3HC1 and its yeast homologue Pml39p to the nuclear basket
por: Gunkel, Philip, et al.
Publicado: (2023)

Enhanced activity of hyperthermostable Pyrococcus horikoshii endoglucanase in superbase ionic liquids
por: Hebal, Hakim, et al.
Publicado: (2022)

Characterization of a Hyperthermostable Alkaline Lipase from Bacillus sonorensis 4R
por: Bhosale, Hemlata, et al.
Publicado: (2016)

Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase
por: Jacquet, Pauline, et al.
Publicado: (2017)

Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme
por: Watkins, Daniel W., et al.
Publicado: (2017)

Non-target Effects of Hyperthermostable α-Amylase Transgenic Nicotiana tabacum in the Laboratory and the Field
por: Scott, Ian Melville, et al.
Publicado: (2019)

Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites
por: Han, Chao, et al.
Publicado: (2020)

Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii
por: Krska, Daniel, et al.
Publicado: (2021)

CDK4 inhibition diminishes p53 activation by MDM2 antagonists
por: Sriraman, Anusha, et al.
Publicado: (2018)

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart
por: Zhang, Shan, et al.
Publicado: (2014)

Increased BST2 expression during simian immunodeficiency virus infection is not a determinant of disease progression in rhesus monkeys
por: Mussil, Bianka, et al.
Publicado: (2015)

Conformational Changes in a Hyperthermostable Glycoside Hydrolase: Enzymatic Activity Is a Consequence of the Loop Dynamics and Protonation Balance
por: de Oliveira, Leandro C., et al.
Publicado: (2015)

Engineering the substrate binding site of the hyperthermostable archaeal endo-β-1,4-galactanase from Ignisphaera aggregans
por: Muderspach, Sebastian J., et al.
Publicado: (2021)

Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A
por: Aksu, Metin, et al.
Publicado: (2016)

Increased APOBEC3G and APOBEC3F expression is associated with low viral load and prolonged survival in simian immunodeficiency virus infected rhesus monkeys
por: Mußil, Bianka, et al.
Publicado: (2011)

The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models
por: Stegmann, Kim M., et al.
Publicado: (2021)

A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies
por: Pleiner, Tino, et al.
Publicado: (2018)

Modular Hyperthermostable Bacterial Endo-β-1,4-Mannanase: Molecular Shape, Flexibility and Temperature-Dependent Conformational Changes
por: da Silva, Viviam M., et al.
Publicado: (2014)

Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m(1)A57/58 methyltransferase
por: Guelorget, Amandine, et al.
Publicado: (2010)

Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library
por: Traxlmayr, Michael W., et al.
Publicado: (2016)

ZC3HC1 is a structural element of the nuclear basket effecting interlinkage of TPR polypeptides
por: Gunkel, Philip, et al.
Publicado: (2022)

Recombinant expression of nanobodies and nanobody-derived immunoreagents
por: de Marco, Ario
Publicado: (2020)

Genotoxic stress increases cytoplasmic mitochondrial DNA editing by human APOBEC3 mutator enzymes at a single cell level
por: Mussil, Bianka, et al.
Publicado: (2019)

CDK4/6 inhibition confers protection of normal gut epithelia against gemcitabine and the active metabolite of irinotecan
por: Blume, Joshua, et al.
Publicado: (2023)

Structural basis for the nuclear import and export functions of the biportin Pdr6/Kap122
por: Aksu, Metin, et al.
Publicado: (2019)

Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation
por: Han, Chao, et al.
Publicado: (2020)

Immobilization of Hyperthermostable Carboxylesterase EstD9 from Anoxybacillus geothermalis D9 onto Polymer Material and Its Physicochemical Properties
por: Johan, Ummie Umaiera Mohd., et al.
Publicado: (2023)

Human APOBEC3A Isoforms Translocate to the Nucleus and Induce DNA Double Strand Breaks Leading to Cell Stress and Death
por: Mussil, Bianka, et al.
Publicado: (2013)

Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide
por: Ng, Sheung Chun, et al.
Publicado: (2021)

Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids
por: Yu, Tianyi, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_q7tsmq3vn9bbl1bdoc74a4p8pg