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Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota

Maternal obesity induces placental dysfunction and intestinal microbial dysbiosis. However, the associations between intestinal microbiota and placental dysfunction are still unclear. In the present study, a gilt model was used to investigate the role of maternal obesity on placental oxidative stres...

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Autores principales: Hu, Chengjun, Yan, Yingli, Ji, Fengjie, Zhou, Hanlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420882/
https://www.ncbi.nlm.nih.gov/pubmed/34497775
http://dx.doi.org/10.3389/fcimb.2021.671347
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author Hu, Chengjun
Yan, Yingli
Ji, Fengjie
Zhou, Hanlin
author_facet Hu, Chengjun
Yan, Yingli
Ji, Fengjie
Zhou, Hanlin
author_sort Hu, Chengjun
collection PubMed
description Maternal obesity induces placental dysfunction and intestinal microbial dysbiosis. However, the associations between intestinal microbiota and placental dysfunction are still unclear. In the present study, a gilt model was used to investigate the role of maternal obesity on placental oxidative stress, mitochondrial function, and fecal microbiota composition, meanwhile identifying microbiota markers associated with placental oxidative stress. Twenty gilts were divided into two groups based on their backfat thickness on parturition day: namely Con group (average backfat thickness = 33 mm), and Obese group (average backfat thickness = 39 mm). The results showed that Obese group was lower than Con group in the birth weight of piglets. Compared with the Con group, the Obesity group exhibited an increased oxidative damage and inflammatory response in placenta, as evidenced by the increased concentrations of placental reactive oxygen species (ROS), protein carboxyl, and interleukin-6 (IL-6). Obesity group was lower than Con group in the concentrations of placental adenosine triphosphate, citrate synthase, and complex I activity. In addition, lower propionate level and Bacteroidetes abundance in feces were seen in the Obese Group. Furthermore, the concentrations of placental ROS, protein carboxyl, and IL-6 were positively correlated with the abundance of Christensenellaceae_R-7_group and negatively correlated with that of norank_f_Bacteroidales_S24-7_group. In conclusion, these findings suggest that maternal obesity might impair oxidative and inflammatory response in placenta through modulating intestinal microbiota composition.
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spelling pubmed-84208822021-09-07 Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota Hu, Chengjun Yan, Yingli Ji, Fengjie Zhou, Hanlin Front Cell Infect Microbiol Cellular and Infection Microbiology Maternal obesity induces placental dysfunction and intestinal microbial dysbiosis. However, the associations between intestinal microbiota and placental dysfunction are still unclear. In the present study, a gilt model was used to investigate the role of maternal obesity on placental oxidative stress, mitochondrial function, and fecal microbiota composition, meanwhile identifying microbiota markers associated with placental oxidative stress. Twenty gilts were divided into two groups based on their backfat thickness on parturition day: namely Con group (average backfat thickness = 33 mm), and Obese group (average backfat thickness = 39 mm). The results showed that Obese group was lower than Con group in the birth weight of piglets. Compared with the Con group, the Obesity group exhibited an increased oxidative damage and inflammatory response in placenta, as evidenced by the increased concentrations of placental reactive oxygen species (ROS), protein carboxyl, and interleukin-6 (IL-6). Obesity group was lower than Con group in the concentrations of placental adenosine triphosphate, citrate synthase, and complex I activity. In addition, lower propionate level and Bacteroidetes abundance in feces were seen in the Obese Group. Furthermore, the concentrations of placental ROS, protein carboxyl, and IL-6 were positively correlated with the abundance of Christensenellaceae_R-7_group and negatively correlated with that of norank_f_Bacteroidales_S24-7_group. In conclusion, these findings suggest that maternal obesity might impair oxidative and inflammatory response in placenta through modulating intestinal microbiota composition. Frontiers Media S.A. 2021-08-23 /pmc/articles/PMC8420882/ /pubmed/34497775 http://dx.doi.org/10.3389/fcimb.2021.671347 Text en Copyright © 2021 Hu, Yan, Ji and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hu, Chengjun
Yan, Yingli
Ji, Fengjie
Zhou, Hanlin
Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title_full Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title_fullStr Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title_full_unstemmed Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title_short Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
title_sort maternal obesity increases oxidative stress in placenta and it is associated with intestinal microbiota
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420882/
https://www.ncbi.nlm.nih.gov/pubmed/34497775
http://dx.doi.org/10.3389/fcimb.2021.671347
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