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Abdominal aortic aneurysm model in swine with bovine pericardium patch

BACKGROUND: Aneurysm repair using endovascular techniques has grown in importance as materials have improved. Studies of endovascular prostheses require experimental models that reproduce anatomic and pathophysiological characteristics of human aneurysms. OBJECTIVES: To describe a porcine model of a...

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Autores principales: Perini, Sílvio César, Bertolucci, Leonardo Henrique, Martins, Ana Paula Donadello, França, Luís Henrique Gil, Aveline, Celso Curcio, Pereira, Adamastor Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV) 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421034/
https://www.ncbi.nlm.nih.gov/pubmed/34527036
http://dx.doi.org/10.1590/1677-5449.210080
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author Perini, Sílvio César
Bertolucci, Leonardo Henrique
Martins, Ana Paula Donadello
França, Luís Henrique Gil
Aveline, Celso Curcio
Pereira, Adamastor Humberto
author_facet Perini, Sílvio César
Bertolucci, Leonardo Henrique
Martins, Ana Paula Donadello
França, Luís Henrique Gil
Aveline, Celso Curcio
Pereira, Adamastor Humberto
author_sort Perini, Sílvio César
collection PubMed
description BACKGROUND: Aneurysm repair using endovascular techniques has grown in importance as materials have improved. Studies of endovascular prostheses require experimental models that reproduce anatomic and pathophysiological characteristics of human aneurysms. OBJECTIVES: To describe a porcine model of abdominal aortic aneurysm. METHODS: This prospective cohort study used eleven Large White female pigs with a mean age of 12 weeks in two study phases. In phase I, the aneurysm was produced with a bovine pericardium patch by retroperitoneal surgery conducted under general anesthesia. In phase II, 15 days later, the animals underwent arteriography and were then euthanized before specimens were removed for histological analysis. RESULTS: Formation of parietal thrombus was observed in all animals. Microscopic analysis showed calcifications around thrombus in 82% of the animals. There was lymphoplasmacytic infiltration in the graft and adjacent area, with fibrosis in nine animals. Three pigs had substantial myointimal thickening, and eight had microcalcifications. Mortality was zero, and there were no ruptures, ischemia, or surgery site infections. CONCLUSIONS: This is a unique model, using inexpensive, biocompatible material. Bovine pericardium is easy for the surgeon to handle and has very similar characteristics to autologous tissue in terms of integration with the cell wall.
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spelling pubmed-84210342021-09-14 Abdominal aortic aneurysm model in swine with bovine pericardium patch Perini, Sílvio César Bertolucci, Leonardo Henrique Martins, Ana Paula Donadello França, Luís Henrique Gil Aveline, Celso Curcio Pereira, Adamastor Humberto J Vasc Bras Original Article BACKGROUND: Aneurysm repair using endovascular techniques has grown in importance as materials have improved. Studies of endovascular prostheses require experimental models that reproduce anatomic and pathophysiological characteristics of human aneurysms. OBJECTIVES: To describe a porcine model of abdominal aortic aneurysm. METHODS: This prospective cohort study used eleven Large White female pigs with a mean age of 12 weeks in two study phases. In phase I, the aneurysm was produced with a bovine pericardium patch by retroperitoneal surgery conducted under general anesthesia. In phase II, 15 days later, the animals underwent arteriography and were then euthanized before specimens were removed for histological analysis. RESULTS: Formation of parietal thrombus was observed in all animals. Microscopic analysis showed calcifications around thrombus in 82% of the animals. There was lymphoplasmacytic infiltration in the graft and adjacent area, with fibrosis in nine animals. Three pigs had substantial myointimal thickening, and eight had microcalcifications. Mortality was zero, and there were no ruptures, ischemia, or surgery site infections. CONCLUSIONS: This is a unique model, using inexpensive, biocompatible material. Bovine pericardium is easy for the surgeon to handle and has very similar characteristics to autologous tissue in terms of integration with the cell wall. Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV) 2021-09-01 /pmc/articles/PMC8421034/ /pubmed/34527036 http://dx.doi.org/10.1590/1677-5449.210080 Text en Copyright© 2021 The authors. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Perini, Sílvio César
Bertolucci, Leonardo Henrique
Martins, Ana Paula Donadello
França, Luís Henrique Gil
Aveline, Celso Curcio
Pereira, Adamastor Humberto
Abdominal aortic aneurysm model in swine with bovine pericardium patch
title Abdominal aortic aneurysm model in swine with bovine pericardium patch
title_full Abdominal aortic aneurysm model in swine with bovine pericardium patch
title_fullStr Abdominal aortic aneurysm model in swine with bovine pericardium patch
title_full_unstemmed Abdominal aortic aneurysm model in swine with bovine pericardium patch
title_short Abdominal aortic aneurysm model in swine with bovine pericardium patch
title_sort abdominal aortic aneurysm model in swine with bovine pericardium patch
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421034/
https://www.ncbi.nlm.nih.gov/pubmed/34527036
http://dx.doi.org/10.1590/1677-5449.210080
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