In-depth assessment of the PAM compatibility and editing activities of Cas9 variants

A series of Cas9 variants have been developed to improve the editing fidelity or targeting range of CRISPR–Cas9. Here, we employ a high-throughput sequencing approach primer-extension-mediated sequencing to analyze the editing efficiency, specificity and protospacer adjacent motif (PAM) compatibilit...

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Detalles Bibliográficos
Autores principales: Zhang, Weiwei, Yin, Jianhang, Zhang-Ding, Zhengrong, Xin, Changchang, Liu, Mengzhu, Wang, Yuhong, Ai, Chen, Hu, Jiazhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421146/
https://www.ncbi.nlm.nih.gov/pubmed/34133740
http://dx.doi.org/10.1093/nar/gkab507

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421146/
https://www.ncbi.nlm.nih.gov/pubmed/34133740
http://dx.doi.org/10.1093/nar/gkab507

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