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Role of a cryptic tRNA gene operon in survival under translational stress

As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tRNAs. Although in most bacterial phyla tRNA genes are dispersed in the genome, many species from distinct phyla also show genes forming arrays. Here, we show that two types of arrays with distinct evolution...

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Autores principales: Santamaría-Gómez, Javier, Rubio, Miguel Ángel, López-Igual, Rocío, Romero-Losada, Ana B, Delgado-Chaves, Fernando M, Bru-Martínez, Roque, Romero-Campero, Francisco J, Herrero, Antonia, Ibba, Michael, Ochoa de Alda, Jesús A G, Luque, Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421152/
https://www.ncbi.nlm.nih.gov/pubmed/34379789
http://dx.doi.org/10.1093/nar/gkab661
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author Santamaría-Gómez, Javier
Rubio, Miguel Ángel
López-Igual, Rocío
Romero-Losada, Ana B
Delgado-Chaves, Fernando M
Bru-Martínez, Roque
Romero-Campero, Francisco J
Herrero, Antonia
Ibba, Michael
Ochoa de Alda, Jesús A G
Luque, Ignacio
author_facet Santamaría-Gómez, Javier
Rubio, Miguel Ángel
López-Igual, Rocío
Romero-Losada, Ana B
Delgado-Chaves, Fernando M
Bru-Martínez, Roque
Romero-Campero, Francisco J
Herrero, Antonia
Ibba, Michael
Ochoa de Alda, Jesús A G
Luque, Ignacio
author_sort Santamaría-Gómez, Javier
collection PubMed
description As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tRNAs. Although in most bacterial phyla tRNA genes are dispersed in the genome, many species from distinct phyla also show genes forming arrays. Here, we show that two types of arrays with distinct evolutionary origins exist. This work focuses on long tRNA gene arrays (L-arrays) that encompass up to 43 genes, which disseminate by horizontal gene transfer and contribute supernumerary tRNA genes to the host. Although in the few cases previously studied these arrays were reported to be poorly transcribed, here we show that the L-array of the model cyanobacterium Anabaena sp. PCC 7120, encoding 23 functional tRNAs, is largely induced upon impairment of the translation machinery. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases. tRNAs encoded in the array are induced in the second phase of the response, directly contributing to cell survival. Results presented here show that in some bacteria the tRNA gene set may be partitioned between a housekeeping subset, which constantly sustains translation, and an inducible subset that is generally silent but can provide functionality under particular conditions.
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spelling pubmed-84211522021-09-09 Role of a cryptic tRNA gene operon in survival under translational stress Santamaría-Gómez, Javier Rubio, Miguel Ángel López-Igual, Rocío Romero-Losada, Ana B Delgado-Chaves, Fernando M Bru-Martínez, Roque Romero-Campero, Francisco J Herrero, Antonia Ibba, Michael Ochoa de Alda, Jesús A G Luque, Ignacio Nucleic Acids Res Molecular Biology As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tRNAs. Although in most bacterial phyla tRNA genes are dispersed in the genome, many species from distinct phyla also show genes forming arrays. Here, we show that two types of arrays with distinct evolutionary origins exist. This work focuses on long tRNA gene arrays (L-arrays) that encompass up to 43 genes, which disseminate by horizontal gene transfer and contribute supernumerary tRNA genes to the host. Although in the few cases previously studied these arrays were reported to be poorly transcribed, here we show that the L-array of the model cyanobacterium Anabaena sp. PCC 7120, encoding 23 functional tRNAs, is largely induced upon impairment of the translation machinery. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases. tRNAs encoded in the array are induced in the second phase of the response, directly contributing to cell survival. Results presented here show that in some bacteria the tRNA gene set may be partitioned between a housekeeping subset, which constantly sustains translation, and an inducible subset that is generally silent but can provide functionality under particular conditions. Oxford University Press 2021-08-11 /pmc/articles/PMC8421152/ /pubmed/34379789 http://dx.doi.org/10.1093/nar/gkab661 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
Santamaría-Gómez, Javier
Rubio, Miguel Ángel
López-Igual, Rocío
Romero-Losada, Ana B
Delgado-Chaves, Fernando M
Bru-Martínez, Roque
Romero-Campero, Francisco J
Herrero, Antonia
Ibba, Michael
Ochoa de Alda, Jesús A G
Luque, Ignacio
Role of a cryptic tRNA gene operon in survival under translational stress
title Role of a cryptic tRNA gene operon in survival under translational stress
title_full Role of a cryptic tRNA gene operon in survival under translational stress
title_fullStr Role of a cryptic tRNA gene operon in survival under translational stress
title_full_unstemmed Role of a cryptic tRNA gene operon in survival under translational stress
title_short Role of a cryptic tRNA gene operon in survival under translational stress
title_sort role of a cryptic trna gene operon in survival under translational stress
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421152/
https://www.ncbi.nlm.nih.gov/pubmed/34379789
http://dx.doi.org/10.1093/nar/gkab661
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