Cargando…

Differential roles of RIG-I like receptors in SARS-CoV-2 infection

Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Duo-Meng, Geng, Ting-Ting, Harrison, Andrew G., Wang, Peng-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421188/
https://www.ncbi.nlm.nih.gov/pubmed/34488908
http://dx.doi.org/10.1186/s40779-021-00340-5
_version_ 1783749025883750400
author Yang, Duo-Meng
Geng, Ting-Ting
Harrison, Andrew G.
Wang, Peng-Hua
author_facet Yang, Duo-Meng
Geng, Ting-Ting
Harrison, Andrew G.
Wang, Peng-Hua
author_sort Yang, Duo-Meng
collection PubMed
description Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A deficiency in MDA5, RIG-I or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication. The expression of the type I/III interferon (IFN) during infection was impaired in MDA5(−/−) and MAVS(−/−), but not in RIG-I(−/−), when compared to wild type (WT) cells. The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2), the cellular entry receptor for SARS-CoV-2, was ~ 2.5-fold higher in RIG-I(−/−) than WT cells. These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor, IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-I in epithelial cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40779-021-00340-5.
format Online
Article
Text
id pubmed-8421188
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-84211882021-09-07 Differential roles of RIG-I like receptors in SARS-CoV-2 infection Yang, Duo-Meng Geng, Ting-Ting Harrison, Andrew G. Wang, Peng-Hua Mil Med Res Letter to the Editor Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A deficiency in MDA5, RIG-I or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication. The expression of the type I/III interferon (IFN) during infection was impaired in MDA5(−/−) and MAVS(−/−), but not in RIG-I(−/−), when compared to wild type (WT) cells. The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2), the cellular entry receptor for SARS-CoV-2, was ~ 2.5-fold higher in RIG-I(−/−) than WT cells. These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor, IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-I in epithelial cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40779-021-00340-5. BioMed Central 2021-09-07 /pmc/articles/PMC8421188/ /pubmed/34488908 http://dx.doi.org/10.1186/s40779-021-00340-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Letter to the Editor
Yang, Duo-Meng
Geng, Ting-Ting
Harrison, Andrew G.
Wang, Peng-Hua
Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title_full Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title_fullStr Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title_full_unstemmed Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title_short Differential roles of RIG-I like receptors in SARS-CoV-2 infection
title_sort differential roles of rig-i like receptors in sars-cov-2 infection
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421188/
https://www.ncbi.nlm.nih.gov/pubmed/34488908
http://dx.doi.org/10.1186/s40779-021-00340-5
work_keys_str_mv AT yangduomeng differentialrolesofrigilikereceptorsinsarscov2infection
AT gengtingting differentialrolesofrigilikereceptorsinsarscov2infection
AT harrisonandrewg differentialrolesofrigilikereceptorsinsarscov2infection
AT wangpenghua differentialrolesofrigilikereceptorsinsarscov2infection