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A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency

CRISPR–Cas9 is a powerful tool for genome engineering, but its efficiency largely depends on guide RNA (gRNA). There are multiple methods available to evaluate the efficiency of gRNAs, including the T7E1 assay, surveyor nuclease assay, deep sequencing, and surrogate reporter systems. In the present...

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Detalles Bibliográficos
Autores principales:	Jung, Soo Bin, Lee, Chae young, Lee, Kwang-Ho, Heo, Kyu, Choi, Si Ho
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421217/ https://www.ncbi.nlm.nih.gov/pubmed/34086942 http://dx.doi.org/10.1093/nar/gkab467

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