TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein

TRIM22 is involved in tumorigenesis and development, but its mechanism is not clear. In this study, we investigated the expression and biological role of TRIM22 in gastric cancer. We found that TRIM22 mRNA and protein expression was abnormally low in gastric cancer tissues and cells and correlated w...

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Autores principales: Zhou, Zhuqing, Gao, Wei, Yuan, Biao, Zhang, Shun, Wang, Kaijing, Du, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421354/
https://www.ncbi.nlm.nih.gov/pubmed/34489426
http://dx.doi.org/10.1038/s41420-021-00627-5
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author Zhou, Zhuqing
Gao, Wei
Yuan, Biao
Zhang, Shun
Wang, Kaijing
Du, Tao
author_facet Zhou, Zhuqing
Gao, Wei
Yuan, Biao
Zhang, Shun
Wang, Kaijing
Du, Tao
author_sort Zhou, Zhuqing
collection PubMed
description TRIM22 is involved in tumorigenesis and development, but its mechanism is not clear. In this study, we investigated the expression and biological role of TRIM22 in gastric cancer. We found that TRIM22 mRNA and protein expression was abnormally low in gastric cancer tissues and cells and correlated with tumor size and depth of invasion. Overexpression of TRIM22 significantly inhibited the proliferation, colony formation, and migration of gastric cancer cells and downregulated the expression of HSPA6. However, the HSPA6-siRNA complementation test showed that TRIM22 did not regulate cell proliferation through HSPA6. Furthermore, overexpression of TRIM22 downregulated the phosphorylation of Smad2 and Smad3. In addition, TRIM22 directly binds to Smad2, and overexpression of Smad2 can reverse the inhibition of cell proliferation and migration induced by TRIM22. In vivo, overexpression of TRIM22 significantly inhibited the growth of subcutaneous xenografts in nude mice. Our study indicates that TRIM22 has an important role in the development of gastric cancer and may inhibit the proliferation of gastric cancer cells through Smad2.
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spelling pubmed-84213542021-09-08 TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein Zhou, Zhuqing Gao, Wei Yuan, Biao Zhang, Shun Wang, Kaijing Du, Tao Cell Death Discov Article TRIM22 is involved in tumorigenesis and development, but its mechanism is not clear. In this study, we investigated the expression and biological role of TRIM22 in gastric cancer. We found that TRIM22 mRNA and protein expression was abnormally low in gastric cancer tissues and cells and correlated with tumor size and depth of invasion. Overexpression of TRIM22 significantly inhibited the proliferation, colony formation, and migration of gastric cancer cells and downregulated the expression of HSPA6. However, the HSPA6-siRNA complementation test showed that TRIM22 did not regulate cell proliferation through HSPA6. Furthermore, overexpression of TRIM22 downregulated the phosphorylation of Smad2 and Smad3. In addition, TRIM22 directly binds to Smad2, and overexpression of Smad2 can reverse the inhibition of cell proliferation and migration induced by TRIM22. In vivo, overexpression of TRIM22 significantly inhibited the growth of subcutaneous xenografts in nude mice. Our study indicates that TRIM22 has an important role in the development of gastric cancer and may inhibit the proliferation of gastric cancer cells through Smad2. Nature Publishing Group UK 2021-09-07 /pmc/articles/PMC8421354/ /pubmed/34489426 http://dx.doi.org/10.1038/s41420-021-00627-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhou, Zhuqing
Gao, Wei
Yuan, Biao
Zhang, Shun
Wang, Kaijing
Du, Tao
TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title_full TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title_fullStr TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title_full_unstemmed TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title_short TRIM22 inhibits the proliferation of gastric cancer cells through the Smad2 protein
title_sort trim22 inhibits the proliferation of gastric cancer cells through the smad2 protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421354/
https://www.ncbi.nlm.nih.gov/pubmed/34489426
http://dx.doi.org/10.1038/s41420-021-00627-5
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