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CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans

BACKGROUND AND OBJECTIVES: Monosomy of chromosome 5 associated with utilization of non-canonical sugar L-sorbose is one of the well-studied aneuploidies in Candida albicans. Stress-induced ploidy changes are crucial determinants for pathogenicity and genetic diversity in C. albicans. The five scatte...

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Autores principales: Pullepu, Dileep, Uddin, Wasim, Narayanan, Aswathy, Kabir, M. Anaul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421577/
https://www.ncbi.nlm.nih.gov/pubmed/34557282
http://dx.doi.org/10.18502/ijm.v13i4.6978
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author Pullepu, Dileep
Uddin, Wasim
Narayanan, Aswathy
Kabir, M. Anaul
author_facet Pullepu, Dileep
Uddin, Wasim
Narayanan, Aswathy
Kabir, M. Anaul
author_sort Pullepu, Dileep
collection PubMed
description BACKGROUND AND OBJECTIVES: Monosomy of chromosome 5 associated with utilization of non-canonical sugar L-sorbose is one of the well-studied aneuploidies in Candida albicans. Stress-induced ploidy changes are crucial determinants for pathogenicity and genetic diversity in C. albicans. The five scattered regulatory regions (A, B, C, 135, and 139) comprising of two functionally redundant pathways (SUR1 and SUR2) were found to be responsible for the growth on L-sorbose. So far, three genes such as CSU51, CSU53 and CSU57 have been identified in region A, region 135 and region C, respectively. In this study we have verified the role of region B in this regulatory pathway. MATERIALS AND METHODS: We employed a combinatorial gene deletion approach to verify the role of region B followed by co-over expression studies and qRT-PCR to identify the regulatory role of this region. RESULTS: We confirmed the role of region B in the regulation of SOU1 gene expression. The qRT-PCR results showed that regulation occurs at transcriptional level along with other two regions in SUR1 pathway. A previously uncharacterized open reading frame in region B has been implicated in this regulation and designated as CSU52. Integrating multiple copies of CSU52 in the genome at tandem, suppresses the growth of recipient strain on L-sorbose, establishing it as a repressor of SOU1 gene. CONCLUSION: This finding completes the identification of regulators in SUR1 pathway. This result paves the way to study the underlying molecular mechanisms of SOU1 gene regulation that in-turn helps to understand stress induced aneuploidy.
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spelling pubmed-84215772021-09-22 CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans Pullepu, Dileep Uddin, Wasim Narayanan, Aswathy Kabir, M. Anaul Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Monosomy of chromosome 5 associated with utilization of non-canonical sugar L-sorbose is one of the well-studied aneuploidies in Candida albicans. Stress-induced ploidy changes are crucial determinants for pathogenicity and genetic diversity in C. albicans. The five scattered regulatory regions (A, B, C, 135, and 139) comprising of two functionally redundant pathways (SUR1 and SUR2) were found to be responsible for the growth on L-sorbose. So far, three genes such as CSU51, CSU53 and CSU57 have been identified in region A, region 135 and region C, respectively. In this study we have verified the role of region B in this regulatory pathway. MATERIALS AND METHODS: We employed a combinatorial gene deletion approach to verify the role of region B followed by co-over expression studies and qRT-PCR to identify the regulatory role of this region. RESULTS: We confirmed the role of region B in the regulation of SOU1 gene expression. The qRT-PCR results showed that regulation occurs at transcriptional level along with other two regions in SUR1 pathway. A previously uncharacterized open reading frame in region B has been implicated in this regulation and designated as CSU52. Integrating multiple copies of CSU52 in the genome at tandem, suppresses the growth of recipient strain on L-sorbose, establishing it as a repressor of SOU1 gene. CONCLUSION: This finding completes the identification of regulators in SUR1 pathway. This result paves the way to study the underlying molecular mechanisms of SOU1 gene regulation that in-turn helps to understand stress induced aneuploidy. Tehran University of Medical Sciences 2021-08 /pmc/articles/PMC8421577/ /pubmed/34557282 http://dx.doi.org/10.18502/ijm.v13i4.6978 Text en Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Pullepu, Dileep
Uddin, Wasim
Narayanan, Aswathy
Kabir, M. Anaul
CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title_full CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title_fullStr CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title_full_unstemmed CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title_short CSU52, a novel regulator functions as a repressor of L-sorbose utilization in Candida albicans
title_sort csu52, a novel regulator functions as a repressor of l-sorbose utilization in candida albicans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421577/
https://www.ncbi.nlm.nih.gov/pubmed/34557282
http://dx.doi.org/10.18502/ijm.v13i4.6978
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