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First Description of a Satellite DNA in Manatees’ Centromeric Regions

Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and...

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Autores principales: Valeri, Mirela Pelizaro, Dias, Guilherme Borges, do Espírito Santo, Alice Alves, Moreira, Camila Nascimento, Yonenaga-Yassuda, Yatiyo, Sommer, Iara Braga, Kuhn, Gustavo C. S., Svartman, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421680/
https://www.ncbi.nlm.nih.gov/pubmed/34504514
http://dx.doi.org/10.3389/fgene.2021.694866
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author Valeri, Mirela Pelizaro
Dias, Guilherme Borges
do Espírito Santo, Alice Alves
Moreira, Camila Nascimento
Yonenaga-Yassuda, Yatiyo
Sommer, Iara Braga
Kuhn, Gustavo C. S.
Svartman, Marta
author_facet Valeri, Mirela Pelizaro
Dias, Guilherme Borges
do Espírito Santo, Alice Alves
Moreira, Camila Nascimento
Yonenaga-Yassuda, Yatiyo
Sommer, Iara Braga
Kuhn, Gustavo C. S.
Svartman, Marta
author_sort Valeri, Mirela Pelizaro
collection PubMed
description Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology.
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spelling pubmed-84216802021-09-08 First Description of a Satellite DNA in Manatees’ Centromeric Regions Valeri, Mirela Pelizaro Dias, Guilherme Borges do Espírito Santo, Alice Alves Moreira, Camila Nascimento Yonenaga-Yassuda, Yatiyo Sommer, Iara Braga Kuhn, Gustavo C. S. Svartman, Marta Front Genet Genetics Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology. Frontiers Media S.A. 2021-08-24 /pmc/articles/PMC8421680/ /pubmed/34504514 http://dx.doi.org/10.3389/fgene.2021.694866 Text en Copyright © 2021 Valeri, Dias, Espírito Santo, Moreira, Yonenaga-Yassuda, Sommer, Kuhn and Svartman. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Valeri, Mirela Pelizaro
Dias, Guilherme Borges
do Espírito Santo, Alice Alves
Moreira, Camila Nascimento
Yonenaga-Yassuda, Yatiyo
Sommer, Iara Braga
Kuhn, Gustavo C. S.
Svartman, Marta
First Description of a Satellite DNA in Manatees’ Centromeric Regions
title First Description of a Satellite DNA in Manatees’ Centromeric Regions
title_full First Description of a Satellite DNA in Manatees’ Centromeric Regions
title_fullStr First Description of a Satellite DNA in Manatees’ Centromeric Regions
title_full_unstemmed First Description of a Satellite DNA in Manatees’ Centromeric Regions
title_short First Description of a Satellite DNA in Manatees’ Centromeric Regions
title_sort first description of a satellite dna in manatees’ centromeric regions
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421680/
https://www.ncbi.nlm.nih.gov/pubmed/34504514
http://dx.doi.org/10.3389/fgene.2021.694866
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