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Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study

Oral mucosal melanomas (OMMs) are aggressive and resistant cancers of high importance in veterinary oncology. Amelanotic OMM produces comparatively less melanin and is considered to be more aggressive than melanotic OMM. Global DNA methylation profiles with hypomethylated or hypermethylated patterns...

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Autores principales: Scattone, Nayra Villar, Epiphanio, Tatiane Moreno Ferrarias, Caddrobi, Karine Germano, Ferrão, Juliana Shimara Pires, Hernandez-Blazquez, Francisco Javier, Loureiro, Ana Paula de Melo, Massoco, Cristina de Oliveira, Dagli, Maria Lucia Zaidan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421724/
https://www.ncbi.nlm.nih.gov/pubmed/34504885
http://dx.doi.org/10.3389/fvets.2021.680181
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author Scattone, Nayra Villar
Epiphanio, Tatiane Moreno Ferrarias
Caddrobi, Karine Germano
Ferrão, Juliana Shimara Pires
Hernandez-Blazquez, Francisco Javier
Loureiro, Ana Paula de Melo
Massoco, Cristina de Oliveira
Dagli, Maria Lucia Zaidan
author_facet Scattone, Nayra Villar
Epiphanio, Tatiane Moreno Ferrarias
Caddrobi, Karine Germano
Ferrão, Juliana Shimara Pires
Hernandez-Blazquez, Francisco Javier
Loureiro, Ana Paula de Melo
Massoco, Cristina de Oliveira
Dagli, Maria Lucia Zaidan
author_sort Scattone, Nayra Villar
collection PubMed
description Oral mucosal melanomas (OMMs) are aggressive and resistant cancers of high importance in veterinary oncology. Amelanotic OMM produces comparatively less melanin and is considered to be more aggressive than melanotic OMM. Global DNA methylation profiles with hypomethylated or hypermethylated patterns have both been associated with aggressive neoplasms; however, global DNA hypomethylation seems to correlate to higher aggressiveness. Accordingly, global DNA methylation in peripheral blood leukocytes has been investigated to understand the role of systemic or environmental factors in cancer development. This study aimed to quantify global DNA methylation in canine melanotic and amelanotic OMM samples and in the peripheral blood leukocytes of the same dogs. Tumor tissue samples were collected from 38 dogs, of which 19 were melanotic and 19 were amelanotic OMM. These were submitted to immunohistochemistry (IHC) with anti-5-methylcytosine (5mC) and anti-Ki67 primary antibodies. Ki67- and 5mC-positive nuclei were manually scored with the help of an image analysis system. Peripheral blood samples were collected from 18 among the 38 OMM-bearing dogs and from 7 additional healthy control dogs. Peripheral blood leukocytes were isolated from the 25 dogs, and DNA was extracted and analyzed by high-performance liquid chromatography (HPLC) for global DNA methylation. The pattern of global DNA methylation in both canine melanotic and amelanotic OMM indicated higher percentages of weakly or negatively stained nuclei in most of the OMM cells, presuming predominant global DNA hypomethylation. In addition, Ki67 counts in amelanotic OMM were significantly higher than those in melanotic OMM (p < 0.001). Global DNA methylation different immunostaining patterns (strong, weak or negative) correlated with Ki67 scores. Global DNA methylation in circulating leukocytes did not differ between the 9 melanotic and 9 amelanotic OMM or between the 18 OMM-bearing dogs and the 7 healthy dogs. This study provides new information on canine melanotic and amelanotic OMM based on global DNA methylation and cell proliferation.
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spelling pubmed-84217242021-09-08 Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study Scattone, Nayra Villar Epiphanio, Tatiane Moreno Ferrarias Caddrobi, Karine Germano Ferrão, Juliana Shimara Pires Hernandez-Blazquez, Francisco Javier Loureiro, Ana Paula de Melo Massoco, Cristina de Oliveira Dagli, Maria Lucia Zaidan Front Vet Sci Veterinary Science Oral mucosal melanomas (OMMs) are aggressive and resistant cancers of high importance in veterinary oncology. Amelanotic OMM produces comparatively less melanin and is considered to be more aggressive than melanotic OMM. Global DNA methylation profiles with hypomethylated or hypermethylated patterns have both been associated with aggressive neoplasms; however, global DNA hypomethylation seems to correlate to higher aggressiveness. Accordingly, global DNA methylation in peripheral blood leukocytes has been investigated to understand the role of systemic or environmental factors in cancer development. This study aimed to quantify global DNA methylation in canine melanotic and amelanotic OMM samples and in the peripheral blood leukocytes of the same dogs. Tumor tissue samples were collected from 38 dogs, of which 19 were melanotic and 19 were amelanotic OMM. These were submitted to immunohistochemistry (IHC) with anti-5-methylcytosine (5mC) and anti-Ki67 primary antibodies. Ki67- and 5mC-positive nuclei were manually scored with the help of an image analysis system. Peripheral blood samples were collected from 18 among the 38 OMM-bearing dogs and from 7 additional healthy control dogs. Peripheral blood leukocytes were isolated from the 25 dogs, and DNA was extracted and analyzed by high-performance liquid chromatography (HPLC) for global DNA methylation. The pattern of global DNA methylation in both canine melanotic and amelanotic OMM indicated higher percentages of weakly or negatively stained nuclei in most of the OMM cells, presuming predominant global DNA hypomethylation. In addition, Ki67 counts in amelanotic OMM were significantly higher than those in melanotic OMM (p < 0.001). Global DNA methylation different immunostaining patterns (strong, weak or negative) correlated with Ki67 scores. Global DNA methylation in circulating leukocytes did not differ between the 9 melanotic and 9 amelanotic OMM or between the 18 OMM-bearing dogs and the 7 healthy dogs. This study provides new information on canine melanotic and amelanotic OMM based on global DNA methylation and cell proliferation. Frontiers Media S.A. 2021-08-24 /pmc/articles/PMC8421724/ /pubmed/34504885 http://dx.doi.org/10.3389/fvets.2021.680181 Text en Copyright © 2021 Scattone, Epiphanio, Caddrobi, Ferrão, Hernandez-Blazquez, Loureiro, Massoco and Dagli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Scattone, Nayra Villar
Epiphanio, Tatiane Moreno Ferrarias
Caddrobi, Karine Germano
Ferrão, Juliana Shimara Pires
Hernandez-Blazquez, Francisco Javier
Loureiro, Ana Paula de Melo
Massoco, Cristina de Oliveira
Dagli, Maria Lucia Zaidan
Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title_full Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title_fullStr Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title_full_unstemmed Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title_short Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study
title_sort quantification of global dna methylation in canine melanotic and amelanotic oral mucosal melanomas and peripheral blood leukocytes from the same patients with omm: first study
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421724/
https://www.ncbi.nlm.nih.gov/pubmed/34504885
http://dx.doi.org/10.3389/fvets.2021.680181
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