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Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit

Bloodstream infection is a life-threatening complication in critically ill patients. Multi-drug resistant bacteria or fungi may increase the risk of invasive infections in hospitalized children and are difficult to treat in intensive care units. The purpose of this study was to use metagenomic next-...

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Autores principales: Yan, Gangfeng, Liu, Jing, Chen, Weiming, Chen, Yang, Cheng, Ye, Tao, Jinhao, Cai, Xiaodi, Zhou, Yang, Wang, Yixue, Wang, Mingbang, Lu, Guoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421769/
https://www.ncbi.nlm.nih.gov/pubmed/34504805
http://dx.doi.org/10.3389/fcimb.2021.665226
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author Yan, Gangfeng
Liu, Jing
Chen, Weiming
Chen, Yang
Cheng, Ye
Tao, Jinhao
Cai, Xiaodi
Zhou, Yang
Wang, Yixue
Wang, Mingbang
Lu, Guoping
author_facet Yan, Gangfeng
Liu, Jing
Chen, Weiming
Chen, Yang
Cheng, Ye
Tao, Jinhao
Cai, Xiaodi
Zhou, Yang
Wang, Yixue
Wang, Mingbang
Lu, Guoping
author_sort Yan, Gangfeng
collection PubMed
description Bloodstream infection is a life-threatening complication in critically ill patients. Multi-drug resistant bacteria or fungi may increase the risk of invasive infections in hospitalized children and are difficult to treat in intensive care units. The purpose of this study was to use metagenomic next-generation sequencing (mNGS) to understand the bloodstream microbiomes of children with suspected sepsis in a pediatric intensive care unit (PICU). mNGS were performed on microbial cell-free nucleic acid from 34 children admitted to PICU, and potentially pathogenic microbes were identified. The associations of serological inflammation indicators, lymphocyte subpopulations, and other clinical phenotypes were also examined. mNGS of blood samples from children in PICU revealed potential eukaryotic microbial pathogens. The abundance of Pneumocystis jirovecii was positively correlated with a decrease in total white blood cell count and immunodeficiency. Hospital-acquired pneumonia patients showed a significant increase in blood bacterial species richness compared with community-acquired pneumonia children. The abundance of bloodstream bacteria was positively correlated with serum procalcitonin level. Microbial genome sequences from potential pathogens were detected in the bloodstream of children with suspected sepsis in PICU, suggesting the presence of bloodstream infections in these children.
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spelling pubmed-84217692021-09-08 Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit Yan, Gangfeng Liu, Jing Chen, Weiming Chen, Yang Cheng, Ye Tao, Jinhao Cai, Xiaodi Zhou, Yang Wang, Yixue Wang, Mingbang Lu, Guoping Front Cell Infect Microbiol Cellular and Infection Microbiology Bloodstream infection is a life-threatening complication in critically ill patients. Multi-drug resistant bacteria or fungi may increase the risk of invasive infections in hospitalized children and are difficult to treat in intensive care units. The purpose of this study was to use metagenomic next-generation sequencing (mNGS) to understand the bloodstream microbiomes of children with suspected sepsis in a pediatric intensive care unit (PICU). mNGS were performed on microbial cell-free nucleic acid from 34 children admitted to PICU, and potentially pathogenic microbes were identified. The associations of serological inflammation indicators, lymphocyte subpopulations, and other clinical phenotypes were also examined. mNGS of blood samples from children in PICU revealed potential eukaryotic microbial pathogens. The abundance of Pneumocystis jirovecii was positively correlated with a decrease in total white blood cell count and immunodeficiency. Hospital-acquired pneumonia patients showed a significant increase in blood bacterial species richness compared with community-acquired pneumonia children. The abundance of bloodstream bacteria was positively correlated with serum procalcitonin level. Microbial genome sequences from potential pathogens were detected in the bloodstream of children with suspected sepsis in PICU, suggesting the presence of bloodstream infections in these children. Frontiers Media S.A. 2021-08-24 /pmc/articles/PMC8421769/ /pubmed/34504805 http://dx.doi.org/10.3389/fcimb.2021.665226 Text en Copyright © 2021 Yan, Liu, Chen, Chen, Cheng, Tao, Cai, Zhou, Wang, Wang and Lu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Yan, Gangfeng
Liu, Jing
Chen, Weiming
Chen, Yang
Cheng, Ye
Tao, Jinhao
Cai, Xiaodi
Zhou, Yang
Wang, Yixue
Wang, Mingbang
Lu, Guoping
Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title_full Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title_fullStr Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title_full_unstemmed Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title_short Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit
title_sort metagenomic next-generation sequencing of bloodstream microbial cell-free nucleic acid in children with suspected sepsis in pediatric intensive care unit
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421769/
https://www.ncbi.nlm.nih.gov/pubmed/34504805
http://dx.doi.org/10.3389/fcimb.2021.665226
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