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Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals

Controlling antibiotic resistance genes (ARGs) is a worldwide intervention to ensure global health. Hospital wastewater is the main pollution source of antibiotic-resistant bacteria and ARGs in the environment. Expanding our knowledge on the bacterial composition of hospital wastewater could help us...

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Autores principales: Guo, Xiurong, Tang, Nan, Lei, Hui, Fang, Qi, Liu, Li, Zhou, Quan, Song, Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421800/
https://www.ncbi.nlm.nih.gov/pubmed/34504480
http://dx.doi.org/10.3389/fmicb.2021.709051
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author Guo, Xiurong
Tang, Nan
Lei, Hui
Fang, Qi
Liu, Li
Zhou, Quan
Song, Can
author_facet Guo, Xiurong
Tang, Nan
Lei, Hui
Fang, Qi
Liu, Li
Zhou, Quan
Song, Can
author_sort Guo, Xiurong
collection PubMed
description Controlling antibiotic resistance genes (ARGs) is a worldwide intervention to ensure global health. Hospital wastewater is the main pollution source of antibiotic-resistant bacteria and ARGs in the environment. Expanding our knowledge on the bacterial composition of hospital wastewater could help us to control infections in hospitals and decrease pathogen release into the environment. In this study, a high-throughput sequencing-based metagenomic approach was applied to investigate the community composition of bacteria and ARGs in untreated wastewater from three different types of hospitals [the general hospital, traditional Chinese medicine (TCM) hospital, and stomatology hospital]. In total, 130 phyla and 2,554 genera were identified from the microbiota of the wastewaters, with significantly different bacterial community compositions among the three hospitals. Total ARG analysis using the Antibiotic Resistance Genes Database (ARDB) and Comprehensive Antibiotic Resistance Database (CARD) revealed that the microbiota in the wastewaters from the three hospitals harbored different types and percentage of ARGs, and their composition was specific to the hospital type based on the correlation analysis between species and ARG abundance, some ARGs contributed to different bacterial genera with various relationships in different hospitals. In summary, our findings demonstrated a widespread occurrence of ARGs and ARG-harboring microbiota in untreated wastewaters of different hospitals, suggesting that protection measures should be applied to prevent human infections. Concurrently, hospital wastewater should be treated more specifically for the removal of pathogens before its discharge into the urban sewage system.
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spelling pubmed-84218002021-09-08 Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals Guo, Xiurong Tang, Nan Lei, Hui Fang, Qi Liu, Li Zhou, Quan Song, Can Front Microbiol Microbiology Controlling antibiotic resistance genes (ARGs) is a worldwide intervention to ensure global health. Hospital wastewater is the main pollution source of antibiotic-resistant bacteria and ARGs in the environment. Expanding our knowledge on the bacterial composition of hospital wastewater could help us to control infections in hospitals and decrease pathogen release into the environment. In this study, a high-throughput sequencing-based metagenomic approach was applied to investigate the community composition of bacteria and ARGs in untreated wastewater from three different types of hospitals [the general hospital, traditional Chinese medicine (TCM) hospital, and stomatology hospital]. In total, 130 phyla and 2,554 genera were identified from the microbiota of the wastewaters, with significantly different bacterial community compositions among the three hospitals. Total ARG analysis using the Antibiotic Resistance Genes Database (ARDB) and Comprehensive Antibiotic Resistance Database (CARD) revealed that the microbiota in the wastewaters from the three hospitals harbored different types and percentage of ARGs, and their composition was specific to the hospital type based on the correlation analysis between species and ARG abundance, some ARGs contributed to different bacterial genera with various relationships in different hospitals. In summary, our findings demonstrated a widespread occurrence of ARGs and ARG-harboring microbiota in untreated wastewaters of different hospitals, suggesting that protection measures should be applied to prevent human infections. Concurrently, hospital wastewater should be treated more specifically for the removal of pathogens before its discharge into the urban sewage system. Frontiers Media S.A. 2021-08-24 /pmc/articles/PMC8421800/ /pubmed/34504480 http://dx.doi.org/10.3389/fmicb.2021.709051 Text en Copyright © 2021 Guo, Tang, Lei, Fang, Liu, Zhou and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Guo, Xiurong
Tang, Nan
Lei, Hui
Fang, Qi
Liu, Li
Zhou, Quan
Song, Can
Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title_full Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title_fullStr Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title_full_unstemmed Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title_short Metagenomic Analysis of Antibiotic Resistance Genes in Untreated Wastewater From Three Different Hospitals
title_sort metagenomic analysis of antibiotic resistance genes in untreated wastewater from three different hospitals
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421800/
https://www.ncbi.nlm.nih.gov/pubmed/34504480
http://dx.doi.org/10.3389/fmicb.2021.709051
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