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TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9
BACKGROUND: Esophageal cancer (EC) is a malignant tumor with high mortality. Correlations have been found between the expression level of tropomyosin 3 (TPM3) and the depth of tumor invasion, lymph node metastasis, and the 5-year survival rate. However, the specific mechanisms underlying EC remain u...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422148/ https://www.ncbi.nlm.nih.gov/pubmed/34532475 http://dx.doi.org/10.21037/atm-21-4043 |
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author | Chen, Sui Shen, Zhimin Gao, Lei Yu, Shaobin Zhang, Peipei Han, Ziyang Kang, Mingqiang |
author_facet | Chen, Sui Shen, Zhimin Gao, Lei Yu, Shaobin Zhang, Peipei Han, Ziyang Kang, Mingqiang |
author_sort | Chen, Sui |
collection | PubMed |
description | BACKGROUND: Esophageal cancer (EC) is a malignant tumor with high mortality. Correlations have been found between the expression level of tropomyosin 3 (TPM3) and the depth of tumor invasion, lymph node metastasis, and the 5-year survival rate. However, the specific mechanisms underlying EC remain unclear. METHODS: Stably transfected TPM3-overexpresing and TPM3-knockdown esophageal squamous cell carcinoma (ESCC) cell lines (ECa109 and EC9706) were constructed, and the association between TPM3 and the proliferation, invasion, and migration of ESCC was investigated using molecular biology methods. The associations between TPM3 and matrix metalloproteinase (MMP)2/9 or epithelial-mesenchymal transition (EMT)-related proteins were verified, and the potential tumor-promoting mechanism was explored by Gelatin Zymography Experiment. RESULTS: TPM3 was found to promote the proliferation, migration, and metastatic potential of ESCC in vivo and in vitro, and stimulate the expression of MMP2/9 and certain EMT markers other than E-cadherin. The replenishment of MMP2/9 restored the malignant behavior of ESCC caused by TPM3. A gelatinase assay showed that the expression of TPM3 was related to the activity of MMP9. CONCLUSIONS: TPM3 promoted proliferation, migration, and metastatic potential in EC cells. Additionally, TPM3 promoted the EMT process. This function may be achieved via the regulation the expression of MMP2/9. |
format | Online Article Text |
id | pubmed-8422148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-84221482021-09-15 TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 Chen, Sui Shen, Zhimin Gao, Lei Yu, Shaobin Zhang, Peipei Han, Ziyang Kang, Mingqiang Ann Transl Med Original Article BACKGROUND: Esophageal cancer (EC) is a malignant tumor with high mortality. Correlations have been found between the expression level of tropomyosin 3 (TPM3) and the depth of tumor invasion, lymph node metastasis, and the 5-year survival rate. However, the specific mechanisms underlying EC remain unclear. METHODS: Stably transfected TPM3-overexpresing and TPM3-knockdown esophageal squamous cell carcinoma (ESCC) cell lines (ECa109 and EC9706) were constructed, and the association between TPM3 and the proliferation, invasion, and migration of ESCC was investigated using molecular biology methods. The associations between TPM3 and matrix metalloproteinase (MMP)2/9 or epithelial-mesenchymal transition (EMT)-related proteins were verified, and the potential tumor-promoting mechanism was explored by Gelatin Zymography Experiment. RESULTS: TPM3 was found to promote the proliferation, migration, and metastatic potential of ESCC in vivo and in vitro, and stimulate the expression of MMP2/9 and certain EMT markers other than E-cadherin. The replenishment of MMP2/9 restored the malignant behavior of ESCC caused by TPM3. A gelatinase assay showed that the expression of TPM3 was related to the activity of MMP9. CONCLUSIONS: TPM3 promoted proliferation, migration, and metastatic potential in EC cells. Additionally, TPM3 promoted the EMT process. This function may be achieved via the regulation the expression of MMP2/9. AME Publishing Company 2021-08 /pmc/articles/PMC8422148/ /pubmed/34532475 http://dx.doi.org/10.21037/atm-21-4043 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Chen, Sui Shen, Zhimin Gao, Lei Yu, Shaobin Zhang, Peipei Han, Ziyang Kang, Mingqiang TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title | TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title_full | TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title_fullStr | TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title_full_unstemmed | TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title_short | TPM3 mediates epithelial-mesenchymal transition in esophageal cancer via MMP2/MMP9 |
title_sort | tpm3 mediates epithelial-mesenchymal transition in esophageal cancer via mmp2/mmp9 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422148/ https://www.ncbi.nlm.nih.gov/pubmed/34532475 http://dx.doi.org/10.21037/atm-21-4043 |
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