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Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis
BACKGROUND: Esophageal cancer is a common malignant tumor and its 5-year survival rate is much lower than 30% due to its invasiveness and pronounced metastasis ability, as well as the difficulty in early diagnosis. This study aimed to elucidate the molecular mechanism of ubiquitin conjugating enzyme...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422647/ https://www.ncbi.nlm.nih.gov/pubmed/34488675 http://dx.doi.org/10.1186/s12885-021-08634-6 |
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author | Li, Rong Pang, Xing-Feng Huang, Zhi-Guang Yang, Li-Hua Peng, Zhi-Gang Ma, Jie He, Rong-Quan |
author_facet | Li, Rong Pang, Xing-Feng Huang, Zhi-Guang Yang, Li-Hua Peng, Zhi-Gang Ma, Jie He, Rong-Quan |
author_sort | Li, Rong |
collection | PubMed |
description | BACKGROUND: Esophageal cancer is a common malignant tumor and its 5-year survival rate is much lower than 30% due to its invasiveness and pronounced metastasis ability, as well as the difficulty in early diagnosis. This study aimed to elucidate the molecular mechanism of ubiquitin conjugating enzyme E2 C (UBE2C) in esophageal squamous cell carcinoma (ESCC). METHODS: In this study, we conducted a comprehensive evaluation of the UBE2C expression in ESCC by collecting the protein and mRNA expression data (including in-house RNA-seq, in-hosue immunohistochemistry, TCGA-GTEx RNA-seq and tissue microarray) to calculate a combined standardized mean difference (SMD) and summary receiver operating characteristic curve (sROC). Kaplan-Meier (K-M) method was used for survival analysis. We also explored the mechanism of UBE2C in ESCC by combing the differentially expressed genes (DEGs) of ESCC, related-genes of UBE2C in ESCC and the putative miRNAs and lncRNAs which may regulate UBE2C. RESULTS: UBE2C protein and mRNA were highly expressed in ESCC tissues (including 772 ESCC tissue samples and 1837 non-cancerous tissue control samples). The pooled SMD of UBE2C expression values was 1.98 (95% CI: 1.51–2.45, p < 0.001), and the the area under the curve (AUC) of the sROC was 0.93 (95% CI: 0.90–0.95). The results of survival analysis suggested that UBE2C is likely to play different roles in different stages of the ESCC. Pathway anaylsis showed that UBE2C mainly influenced the biological function of esophageal cancer by synergistic effects with CDK1, PTTG1 and SKP2. We also constructed a potential UBE2C-related ceRNA network for ESCC (HCP5/has-miR-139-5p/UBE2C). CONCLUSION: UBE2C mRNA and protein level were highly expressed in ESCC and UBE2C was likely to play different roles in different stages of the ESCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-021-08634-6. |
format | Online Article Text |
id | pubmed-8422647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-84226472021-09-09 Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis Li, Rong Pang, Xing-Feng Huang, Zhi-Guang Yang, Li-Hua Peng, Zhi-Gang Ma, Jie He, Rong-Quan BMC Cancer Research BACKGROUND: Esophageal cancer is a common malignant tumor and its 5-year survival rate is much lower than 30% due to its invasiveness and pronounced metastasis ability, as well as the difficulty in early diagnosis. This study aimed to elucidate the molecular mechanism of ubiquitin conjugating enzyme E2 C (UBE2C) in esophageal squamous cell carcinoma (ESCC). METHODS: In this study, we conducted a comprehensive evaluation of the UBE2C expression in ESCC by collecting the protein and mRNA expression data (including in-house RNA-seq, in-hosue immunohistochemistry, TCGA-GTEx RNA-seq and tissue microarray) to calculate a combined standardized mean difference (SMD) and summary receiver operating characteristic curve (sROC). Kaplan-Meier (K-M) method was used for survival analysis. We also explored the mechanism of UBE2C in ESCC by combing the differentially expressed genes (DEGs) of ESCC, related-genes of UBE2C in ESCC and the putative miRNAs and lncRNAs which may regulate UBE2C. RESULTS: UBE2C protein and mRNA were highly expressed in ESCC tissues (including 772 ESCC tissue samples and 1837 non-cancerous tissue control samples). The pooled SMD of UBE2C expression values was 1.98 (95% CI: 1.51–2.45, p < 0.001), and the the area under the curve (AUC) of the sROC was 0.93 (95% CI: 0.90–0.95). The results of survival analysis suggested that UBE2C is likely to play different roles in different stages of the ESCC. Pathway anaylsis showed that UBE2C mainly influenced the biological function of esophageal cancer by synergistic effects with CDK1, PTTG1 and SKP2. We also constructed a potential UBE2C-related ceRNA network for ESCC (HCP5/has-miR-139-5p/UBE2C). CONCLUSION: UBE2C mRNA and protein level were highly expressed in ESCC and UBE2C was likely to play different roles in different stages of the ESCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-021-08634-6. BioMed Central 2021-09-06 /pmc/articles/PMC8422647/ /pubmed/34488675 http://dx.doi.org/10.1186/s12885-021-08634-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Li, Rong Pang, Xing-Feng Huang, Zhi-Guang Yang, Li-Hua Peng, Zhi-Gang Ma, Jie He, Rong-Quan Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title | Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title_full | Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title_fullStr | Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title_full_unstemmed | Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title_short | Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis |
title_sort | overexpression of ube2c in esophageal squamous cell carcinoma tissues and molecular analysis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422647/ https://www.ncbi.nlm.nih.gov/pubmed/34488675 http://dx.doi.org/10.1186/s12885-021-08634-6 |
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