Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was char...

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Detalles Bibliográficos
Autores principales: Lau, Skadi, Liu, Yue, Maier, Anna, Braune, Steffen, Gossen, Manfred, Neffe, Axel T., Lendlein, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422954/
https://www.ncbi.nlm.nih.gov/pubmed/34513262
http://dx.doi.org/10.1557/s43579-021-00072-6
Descripción
Sumario:In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00072-6.

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