Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was char...

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Autores principales: Lau, Skadi, Liu, Yue, Maier, Anna, Braune, Steffen, Gossen, Manfred, Neffe, Axel T., Lendlein, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422954/
https://www.ncbi.nlm.nih.gov/pubmed/34513262
http://dx.doi.org/10.1557/s43579-021-00072-6
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author Lau, Skadi
Liu, Yue
Maier, Anna
Braune, Steffen
Gossen, Manfred
Neffe, Axel T.
Lendlein, Andreas
author_facet Lau, Skadi
Liu, Yue
Maier, Anna
Braune, Steffen
Gossen, Manfred
Neffe, Axel T.
Lendlein, Andreas
author_sort Lau, Skadi
collection PubMed
description In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00072-6.
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spelling pubmed-84229542021-09-08 Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation Lau, Skadi Liu, Yue Maier, Anna Braune, Steffen Gossen, Manfred Neffe, Axel T. Lendlein, Andreas MRS Commun Research Letter In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00072-6. Springer International Publishing 2021-09-07 2021 /pmc/articles/PMC8422954/ /pubmed/34513262 http://dx.doi.org/10.1557/s43579-021-00072-6 Text en © Helmholtz-Zentrum Hereon 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Letter
Lau, Skadi
Liu, Yue
Maier, Anna
Braune, Steffen
Gossen, Manfred
Neffe, Axel T.
Lendlein, Andreas
Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title_full Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title_fullStr Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title_full_unstemmed Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title_short Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
title_sort establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422954/
https://www.ncbi.nlm.nih.gov/pubmed/34513262
http://dx.doi.org/10.1557/s43579-021-00072-6
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