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Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital

BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAB) is among the most concerning cause of healthcare-associated infections (HAI) due to its high level of antibiotic resistance and high mortality. In the era of the COVID-19 pandemic, the key priority of infection control committees is to...

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Autores principales: Abdollahi, Alireza, Aliramezani, Amir, Salehi, Mohammadreza, Norouzi Shadehi, Mahsa, Ghourchian, Sedighe, Douraghi, Masoumeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423834/
https://www.ncbi.nlm.nih.gov/pubmed/34496774
http://dx.doi.org/10.1186/s12879-021-06642-2
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author Abdollahi, Alireza
Aliramezani, Amir
Salehi, Mohammadreza
Norouzi Shadehi, Mahsa
Ghourchian, Sedighe
Douraghi, Masoumeh
author_facet Abdollahi, Alireza
Aliramezani, Amir
Salehi, Mohammadreza
Norouzi Shadehi, Mahsa
Ghourchian, Sedighe
Douraghi, Masoumeh
author_sort Abdollahi, Alireza
collection PubMed
description BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAB) is among the most concerning cause of healthcare-associated infections (HAI) due to its high level of antibiotic resistance and high mortality. In the era of the COVID-19 pandemic, the key priority of infection control committees is to contain the dissemination of antibiotic resistant Gram-negative bacteria. Here, we aimed to timely recognize the emergence of CRAB in COVID-19 cases admitted to the wards of a tertiary referral hospital and to identify the genetic relatedness of the isolates. METHODS: From 30 March to 30 May 2020, a total of 242 clinical samples from COVID-19 cases were screened for CRAB isolates using standard microbiologic and antibiotic susceptibility tests. The PCRs targeting oxa23, oxa24, oxa58, bla(TEM) and bla(NDM-1) genes were performed. Two multiplex PCRs for identifying the global clones (GC) of A. baumannii were also performed. The sequence type of CRABs was determined using Institut Pasteur (IP) multilocus sequence typing (MLST) scheme. RESULTS: Eighteen CRAB isolates were recovered from COVID-19 patients with the mean age of 63.94 ± 13.8 years. All but 4 COVID-19 patients co-infected with CRAB were suffering from an underlying disease. Death was recorded as the outcome in ICUs for 9 (50%) COVID-19 patients co-infected with CRAB. The CRAB isolates belong to GC2 and ST2(IP) and carried the oxa23 carbapenem resistance gene. CONCLUSION: This study demonstrated the co-infection of CRAB isolates and SARS-CoV-2 in the patients admitted to different ICUs at a referral hospital in Tehran. The CRAB isolates were found to belong to ST2(IP), share the oxa23 gene and to have caused several outbreaks in the wards admitting COVID-19 patients.
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spelling pubmed-84238342021-09-08 Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital Abdollahi, Alireza Aliramezani, Amir Salehi, Mohammadreza Norouzi Shadehi, Mahsa Ghourchian, Sedighe Douraghi, Masoumeh BMC Infect Dis Research BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAB) is among the most concerning cause of healthcare-associated infections (HAI) due to its high level of antibiotic resistance and high mortality. In the era of the COVID-19 pandemic, the key priority of infection control committees is to contain the dissemination of antibiotic resistant Gram-negative bacteria. Here, we aimed to timely recognize the emergence of CRAB in COVID-19 cases admitted to the wards of a tertiary referral hospital and to identify the genetic relatedness of the isolates. METHODS: From 30 March to 30 May 2020, a total of 242 clinical samples from COVID-19 cases were screened for CRAB isolates using standard microbiologic and antibiotic susceptibility tests. The PCRs targeting oxa23, oxa24, oxa58, bla(TEM) and bla(NDM-1) genes were performed. Two multiplex PCRs for identifying the global clones (GC) of A. baumannii were also performed. The sequence type of CRABs was determined using Institut Pasteur (IP) multilocus sequence typing (MLST) scheme. RESULTS: Eighteen CRAB isolates were recovered from COVID-19 patients with the mean age of 63.94 ± 13.8 years. All but 4 COVID-19 patients co-infected with CRAB were suffering from an underlying disease. Death was recorded as the outcome in ICUs for 9 (50%) COVID-19 patients co-infected with CRAB. The CRAB isolates belong to GC2 and ST2(IP) and carried the oxa23 carbapenem resistance gene. CONCLUSION: This study demonstrated the co-infection of CRAB isolates and SARS-CoV-2 in the patients admitted to different ICUs at a referral hospital in Tehran. The CRAB isolates were found to belong to ST2(IP), share the oxa23 gene and to have caused several outbreaks in the wards admitting COVID-19 patients. BioMed Central 2021-09-08 /pmc/articles/PMC8423834/ /pubmed/34496774 http://dx.doi.org/10.1186/s12879-021-06642-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Abdollahi, Alireza
Aliramezani, Amir
Salehi, Mohammadreza
Norouzi Shadehi, Mahsa
Ghourchian, Sedighe
Douraghi, Masoumeh
Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title_full Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title_fullStr Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title_full_unstemmed Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title_short Co-infection of ST2(IP) carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital
title_sort co-infection of st2(ip) carbapenem-resistant acinetobacter baumannii with sars-cov-2 in the patients admitted to a tehran tertiary referral hospital
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423834/
https://www.ncbi.nlm.nih.gov/pubmed/34496774
http://dx.doi.org/10.1186/s12879-021-06642-2
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