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Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria

Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of t...

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Autores principales: Liu, Yundan, Zheng, Kaiyang, Liu, Baohong, Liang, Yantao, You, Siyuan, Zhang, Wenjing, Zhang, Xinran, Jie, Yaqi, Shao, Hongbing, Jiang, Yong, Guo, Cui, He, Hui, Wang, Hualong, Sung, Yeong Yik, Mok, Wen Jye, Wong, Li Lian, McMinn, Andrew, Wang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424206/
https://www.ncbi.nlm.nih.gov/pubmed/34512604
http://dx.doi.org/10.3389/fmicb.2021.726074
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author Liu, Yundan
Zheng, Kaiyang
Liu, Baohong
Liang, Yantao
You, Siyuan
Zhang, Wenjing
Zhang, Xinran
Jie, Yaqi
Shao, Hongbing
Jiang, Yong
Guo, Cui
He, Hui
Wang, Hualong
Sung, Yeong Yik
Mok, Wen Jye
Wong, Li Lian
McMinn, Andrew
Wang, Min
author_facet Liu, Yundan
Zheng, Kaiyang
Liu, Baohong
Liang, Yantao
You, Siyuan
Zhang, Wenjing
Zhang, Xinran
Jie, Yaqi
Shao, Hongbing
Jiang, Yong
Guo, Cui
He, Hui
Wang, Hualong
Sung, Yeong Yik
Mok, Wen Jye
Wong, Li Lian
McMinn, Andrew
Wang, Min
author_sort Liu, Yundan
collection PubMed
description Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as Marinovirus. This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean.
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spelling pubmed-84242062021-09-09 Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria Liu, Yundan Zheng, Kaiyang Liu, Baohong Liang, Yantao You, Siyuan Zhang, Wenjing Zhang, Xinran Jie, Yaqi Shao, Hongbing Jiang, Yong Guo, Cui He, Hui Wang, Hualong Sung, Yeong Yik Mok, Wen Jye Wong, Li Lian McMinn, Andrew Wang, Min Front Microbiol Microbiology Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as Marinovirus. This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean. Frontiers Media S.A. 2021-08-25 /pmc/articles/PMC8424206/ /pubmed/34512604 http://dx.doi.org/10.3389/fmicb.2021.726074 Text en Copyright © 2021 Liu, Zheng, Liu, Liang, You, Zhang, Zhang, Jie, Shao, Jiang, Guo, He, Wang, Sung, Mok, Wong, McMinn and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yundan
Zheng, Kaiyang
Liu, Baohong
Liang, Yantao
You, Siyuan
Zhang, Wenjing
Zhang, Xinran
Jie, Yaqi
Shao, Hongbing
Jiang, Yong
Guo, Cui
He, Hui
Wang, Hualong
Sung, Yeong Yik
Mok, Wen Jye
Wong, Li Lian
McMinn, Andrew
Wang, Min
Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title_full Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title_fullStr Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title_full_unstemmed Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title_short Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria
title_sort characterization and genomic analysis of marinobacter phage vb_mals-ps3, representing a new lambda-like temperate siphoviral genus infecting algae-associated bacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424206/
https://www.ncbi.nlm.nih.gov/pubmed/34512604
http://dx.doi.org/10.3389/fmicb.2021.726074
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