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LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop
BACKGROUND: Lung adenocarcinoma (LUAD) is the most common histological subtype of primary lung cancer. To identify the biomarker of diagnosis for LUAD is of great significance. Long non-coding RNAs (lncRNAs) were previously revealed to exert vital effects in numerous cancers. LncRNA long intergenic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425021/ https://www.ncbi.nlm.nih.gov/pubmed/34496829 http://dx.doi.org/10.1186/s12890-021-01657-6 |
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author | Huang, Wen Wang, Xinxing Wu, Fubing Xu, Fanggui |
author_facet | Huang, Wen Wang, Xinxing Wu, Fubing Xu, Fanggui |
author_sort | Huang, Wen |
collection | PubMed |
description | BACKGROUND: Lung adenocarcinoma (LUAD) is the most common histological subtype of primary lung cancer. To identify the biomarker of diagnosis for LUAD is of great significance. Long non-coding RNAs (lncRNAs) were previously revealed to exert vital effects in numerous cancers. LncRNA long intergenic non-protein coding RNA 520 (LINC00520) served as an oncogene in various cancers. Therefore, our study was specially designed to probe the role of LINC00520 in LUAD. RESULTS: LINC00520 expression was detected by RT-qPCR. Next, function of LINC00520 in LUAD was verified by in vitro loss-of-function experiments. DNA pull down, ChIP, RIP, and luciferase reporter assays were conducted to reveal the regulatory mechanism of LINC00520. We found that LINC00520 was upregulated in LUAD. Additionally, LINC00520 upregulation is associated with the poor prognosis for patients with LUAD. Furthermore, LINC00520 downregulation suppressed LUAD cell proliferation and migration and induced cell apoptosis. Forkhead box P3 (FOXP3) is identified as the transcription factor to transcriptionally activate LINC00520. Moreover, LINC00520 positively upregulated FOXP3 expression via sponging miR-3611 in LUAD cells. Subsequently, rescue experiments delineated that miR-3611 downregulation or FOXP3 overexpression reversed the effects of silenced LINC00520 on proliferative and migratory capabilities in LUAD cells. CONCLUSION: This study innovatively indicated that lncRNA LINC00520 facilitated cell proliferative and migratory abilities in LUAD through interacting with miR-3611 and targeting FOXP3, which may provide a potential novel insight for treatment of LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-021-01657-6. |
format | Online Article Text |
id | pubmed-8425021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-84250212021-09-08 LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop Huang, Wen Wang, Xinxing Wu, Fubing Xu, Fanggui BMC Pulm Med Research BACKGROUND: Lung adenocarcinoma (LUAD) is the most common histological subtype of primary lung cancer. To identify the biomarker of diagnosis for LUAD is of great significance. Long non-coding RNAs (lncRNAs) were previously revealed to exert vital effects in numerous cancers. LncRNA long intergenic non-protein coding RNA 520 (LINC00520) served as an oncogene in various cancers. Therefore, our study was specially designed to probe the role of LINC00520 in LUAD. RESULTS: LINC00520 expression was detected by RT-qPCR. Next, function of LINC00520 in LUAD was verified by in vitro loss-of-function experiments. DNA pull down, ChIP, RIP, and luciferase reporter assays were conducted to reveal the regulatory mechanism of LINC00520. We found that LINC00520 was upregulated in LUAD. Additionally, LINC00520 upregulation is associated with the poor prognosis for patients with LUAD. Furthermore, LINC00520 downregulation suppressed LUAD cell proliferation and migration and induced cell apoptosis. Forkhead box P3 (FOXP3) is identified as the transcription factor to transcriptionally activate LINC00520. Moreover, LINC00520 positively upregulated FOXP3 expression via sponging miR-3611 in LUAD cells. Subsequently, rescue experiments delineated that miR-3611 downregulation or FOXP3 overexpression reversed the effects of silenced LINC00520 on proliferative and migratory capabilities in LUAD cells. CONCLUSION: This study innovatively indicated that lncRNA LINC00520 facilitated cell proliferative and migratory abilities in LUAD through interacting with miR-3611 and targeting FOXP3, which may provide a potential novel insight for treatment of LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-021-01657-6. BioMed Central 2021-09-08 /pmc/articles/PMC8425021/ /pubmed/34496829 http://dx.doi.org/10.1186/s12890-021-01657-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Huang, Wen Wang, Xinxing Wu, Fubing Xu, Fanggui LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title | LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title_full | LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title_fullStr | LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title_full_unstemmed | LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title_short | LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
title_sort | lncrna linc00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425021/ https://www.ncbi.nlm.nih.gov/pubmed/34496829 http://dx.doi.org/10.1186/s12890-021-01657-6 |
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