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FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2
Background and Aim: Some studies have verified that miR-133a played an inhibitory role in several cancers. Whereas, the effect of miRNA-133a in colorectal cancer (CRC) has not been fully elucidated. Our study aims to confirm UBA2 as a direct target gene of miRNA-133a and explore the upstream modulat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425194/ https://www.ncbi.nlm.nih.gov/pubmed/34539887 http://dx.doi.org/10.7150/jca.60647 |
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author | Cheng, Yuanfang Wang, Yajuan Cheng, Yuanzun Yang, Quanzhong Zhang, Lejing Li, Zhaoxi Cheng, Jiancheng |
author_facet | Cheng, Yuanfang Wang, Yajuan Cheng, Yuanzun Yang, Quanzhong Zhang, Lejing Li, Zhaoxi Cheng, Jiancheng |
author_sort | Cheng, Yuanfang |
collection | PubMed |
description | Background and Aim: Some studies have verified that miR-133a played an inhibitory role in several cancers. Whereas, the effect of miRNA-133a in colorectal cancer (CRC) has not been fully elucidated. Our study aims to confirm UBA2 as a direct target gene of miRNA-133a and explore the upstream modulatory molecules of miR-133a. In addition, their impacts on the biological characteristics of CRC cells were assessed. Methods: QRT-PCR analyzed miR-133a expression levels in colorectal cells including HCT116, SW48 cells and human normal colorectal cell line NCM460. A serial biological experiment assessed miR-133a effects on cell proliferation, migration, invasion and apoptosis capacities in HCT116 and SW48 cells. MiRNA targeting gene prediction and a dual luciferase assay were employed to confirm miR-133a-targeted UBA2. Transcription factors (TFs) FOXD3 was identified as an upstream regulator of miR-133a via JASPAR. The influence of miR-133a and FOXD3 on UBA2 expression was analyzed by qRT-PCR or western blot. Results: miR-133a was lowly expressed in CRC cells. High miRNA-133a expression suppressed the proliferation, migration, invasion and enhanced apoptosis capacities of CRC cells. MiR-133a targeted the UBA2 mRNA 3ʹUTR area and reduced UBA2 protein expression. We also unveiled that FOXD3 high-expression significantly raised miR-133a expression and diminished UBA2 expression. We also discovered that high miR-133a expression augmented the effects of elevated FOXD3 expression on CRC cell proliferation, migration and invasion, whereas, low miR-133a expression generated the opposite outcomes. Conclusion: FOXD3 induced miRNA-133a directly targeting UBA2 could affect the progression and growth of CRC. |
format | Online Article Text |
id | pubmed-8425194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-84251942021-09-16 FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 Cheng, Yuanfang Wang, Yajuan Cheng, Yuanzun Yang, Quanzhong Zhang, Lejing Li, Zhaoxi Cheng, Jiancheng J Cancer Research Paper Background and Aim: Some studies have verified that miR-133a played an inhibitory role in several cancers. Whereas, the effect of miRNA-133a in colorectal cancer (CRC) has not been fully elucidated. Our study aims to confirm UBA2 as a direct target gene of miRNA-133a and explore the upstream modulatory molecules of miR-133a. In addition, their impacts on the biological characteristics of CRC cells were assessed. Methods: QRT-PCR analyzed miR-133a expression levels in colorectal cells including HCT116, SW48 cells and human normal colorectal cell line NCM460. A serial biological experiment assessed miR-133a effects on cell proliferation, migration, invasion and apoptosis capacities in HCT116 and SW48 cells. MiRNA targeting gene prediction and a dual luciferase assay were employed to confirm miR-133a-targeted UBA2. Transcription factors (TFs) FOXD3 was identified as an upstream regulator of miR-133a via JASPAR. The influence of miR-133a and FOXD3 on UBA2 expression was analyzed by qRT-PCR or western blot. Results: miR-133a was lowly expressed in CRC cells. High miRNA-133a expression suppressed the proliferation, migration, invasion and enhanced apoptosis capacities of CRC cells. MiR-133a targeted the UBA2 mRNA 3ʹUTR area and reduced UBA2 protein expression. We also unveiled that FOXD3 high-expression significantly raised miR-133a expression and diminished UBA2 expression. We also discovered that high miR-133a expression augmented the effects of elevated FOXD3 expression on CRC cell proliferation, migration and invasion, whereas, low miR-133a expression generated the opposite outcomes. Conclusion: FOXD3 induced miRNA-133a directly targeting UBA2 could affect the progression and growth of CRC. Ivyspring International Publisher 2021-08-25 /pmc/articles/PMC8425194/ /pubmed/34539887 http://dx.doi.org/10.7150/jca.60647 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Cheng, Yuanfang Wang, Yajuan Cheng, Yuanzun Yang, Quanzhong Zhang, Lejing Li, Zhaoxi Cheng, Jiancheng FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title | FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title_full | FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title_fullStr | FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title_full_unstemmed | FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title_short | FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2 |
title_sort | foxd3-induced mir-133a blocks progression and metastasis of colorectal cancer through regulating uba2 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425194/ https://www.ncbi.nlm.nih.gov/pubmed/34539887 http://dx.doi.org/10.7150/jca.60647 |
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