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Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system
Trigeminal (TG), dorsal root (DRG), and nodose/jugular (NG/JG) ganglia each possess specialized and distinct functions. We used RNA sequencing of two-cycle sorted Pirt-positive neurons to identify genes exclusively expressing in L3–L5 DRG, T10-L1 DRG, NG/JG, and TG mouse ganglion neurons. Transcript...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426369/ https://www.ncbi.nlm.nih.gov/pubmed/34497285 http://dx.doi.org/10.1038/s41598-021-97084-y |
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author | Hovhannisyan, Anahit H. Son, Hyeonwi Mecklenburg, Jennifer Barba-Escobedo, Priscilla Ann Tram, Meilinn Gomez, Ruben Shannonhouse, John Zou, Yi Weldon, Korri Ruparel, Shivani Lai, Zhao Tumanov, Alexei V. Kim, Yu Shin Akopian, Armen N. |
author_facet | Hovhannisyan, Anahit H. Son, Hyeonwi Mecklenburg, Jennifer Barba-Escobedo, Priscilla Ann Tram, Meilinn Gomez, Ruben Shannonhouse, John Zou, Yi Weldon, Korri Ruparel, Shivani Lai, Zhao Tumanov, Alexei V. Kim, Yu Shin Akopian, Armen N. |
author_sort | Hovhannisyan, Anahit H. |
collection | PubMed |
description | Trigeminal (TG), dorsal root (DRG), and nodose/jugular (NG/JG) ganglia each possess specialized and distinct functions. We used RNA sequencing of two-cycle sorted Pirt-positive neurons to identify genes exclusively expressing in L3–L5 DRG, T10-L1 DRG, NG/JG, and TG mouse ganglion neurons. Transcription factor Phox2b and Efcab6 are specifically expressed in NG/JG while Hoxa7 is exclusively present in both T10-L1 and L3–L5 DRG neurons. Cyp2f2, Krt18, and Ptgds, along with pituitary hormone prolactin (Prl), growth hormone (Gh), and proopiomelanocortin (Pomc) encoding genes are almost exclusively in TG neurons. Immunohistochemistry confirmed selective expression of these hormones in TG neurons and dural nerves; and showed GH expression in subsets of TRPV1(+) and CGRP(+) TG neurons. We next examined GH roles in hypersensitivity in the spinal versus trigeminal systems. Exogenous GH produced mechanical hypersensitivity when injected intrathecally, but not intraplantarly. GH-induced thermal hypersensitivity was not detected in the spinal system. GH dose-dependently generated orofacial and headache-like periorbital mechanical hypersensitivity after administration into masseter muscle and dura, respectively. Periorbital mechanical hypersensitivity was reversed by a GH receptor antagonist, pegvisomant. Overall, pituitary hormone genes are selective for TG versus other ganglia somatotypes; and GH has distinctive functional significance in the trigeminal versus spinal systems. |
format | Online Article Text |
id | pubmed-8426369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84263692021-09-09 Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system Hovhannisyan, Anahit H. Son, Hyeonwi Mecklenburg, Jennifer Barba-Escobedo, Priscilla Ann Tram, Meilinn Gomez, Ruben Shannonhouse, John Zou, Yi Weldon, Korri Ruparel, Shivani Lai, Zhao Tumanov, Alexei V. Kim, Yu Shin Akopian, Armen N. Sci Rep Article Trigeminal (TG), dorsal root (DRG), and nodose/jugular (NG/JG) ganglia each possess specialized and distinct functions. We used RNA sequencing of two-cycle sorted Pirt-positive neurons to identify genes exclusively expressing in L3–L5 DRG, T10-L1 DRG, NG/JG, and TG mouse ganglion neurons. Transcription factor Phox2b and Efcab6 are specifically expressed in NG/JG while Hoxa7 is exclusively present in both T10-L1 and L3–L5 DRG neurons. Cyp2f2, Krt18, and Ptgds, along with pituitary hormone prolactin (Prl), growth hormone (Gh), and proopiomelanocortin (Pomc) encoding genes are almost exclusively in TG neurons. Immunohistochemistry confirmed selective expression of these hormones in TG neurons and dural nerves; and showed GH expression in subsets of TRPV1(+) and CGRP(+) TG neurons. We next examined GH roles in hypersensitivity in the spinal versus trigeminal systems. Exogenous GH produced mechanical hypersensitivity when injected intrathecally, but not intraplantarly. GH-induced thermal hypersensitivity was not detected in the spinal system. GH dose-dependently generated orofacial and headache-like periorbital mechanical hypersensitivity after administration into masseter muscle and dura, respectively. Periorbital mechanical hypersensitivity was reversed by a GH receptor antagonist, pegvisomant. Overall, pituitary hormone genes are selective for TG versus other ganglia somatotypes; and GH has distinctive functional significance in the trigeminal versus spinal systems. Nature Publishing Group UK 2021-09-08 /pmc/articles/PMC8426369/ /pubmed/34497285 http://dx.doi.org/10.1038/s41598-021-97084-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hovhannisyan, Anahit H. Son, Hyeonwi Mecklenburg, Jennifer Barba-Escobedo, Priscilla Ann Tram, Meilinn Gomez, Ruben Shannonhouse, John Zou, Yi Weldon, Korri Ruparel, Shivani Lai, Zhao Tumanov, Alexei V. Kim, Yu Shin Akopian, Armen N. Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title | Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title_full | Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title_fullStr | Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title_full_unstemmed | Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title_short | Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
title_sort | pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426369/ https://www.ncbi.nlm.nih.gov/pubmed/34497285 http://dx.doi.org/10.1038/s41598-021-97084-y |
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