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Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study

Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transge...

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Autores principales: Matchynski, James I., Manwar, Rayyan, Kratkiewicz, Karl J., Madangopal, Rajtarun, Lennon, Veronica A., Makki, Kassem M., Reppen, Abbey L., Woznicki, Alexander R., Hope, Bruce T., Perrine, Shane A., Conti, Alana C., Avanaki, Kamran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426561/
https://www.ncbi.nlm.nih.gov/pubmed/34522608
http://dx.doi.org/10.1016/j.pacs.2021.100297
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author Matchynski, James I.
Manwar, Rayyan
Kratkiewicz, Karl J.
Madangopal, Rajtarun
Lennon, Veronica A.
Makki, Kassem M.
Reppen, Abbey L.
Woznicki, Alexander R.
Hope, Bruce T.
Perrine, Shane A.
Conti, Alana C.
Avanaki, Kamran
author_facet Matchynski, James I.
Manwar, Rayyan
Kratkiewicz, Karl J.
Madangopal, Rajtarun
Lennon, Veronica A.
Makki, Kassem M.
Reppen, Abbey L.
Woznicki, Alexander R.
Hope, Bruce T.
Perrine, Shane A.
Conti, Alana C.
Avanaki, Kamran
author_sort Matchynski, James I.
collection PubMed
description Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large field-of-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an ex vivo imaging method in the Wistar Fos-LacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fos-expressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction.
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spelling pubmed-84265612021-09-13 Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study Matchynski, James I. Manwar, Rayyan Kratkiewicz, Karl J. Madangopal, Rajtarun Lennon, Veronica A. Makki, Kassem M. Reppen, Abbey L. Woznicki, Alexander R. Hope, Bruce T. Perrine, Shane A. Conti, Alana C. Avanaki, Kamran Photoacoustics Research Article Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large field-of-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an ex vivo imaging method in the Wistar Fos-LacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fos-expressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction. Elsevier 2021-08-30 /pmc/articles/PMC8426561/ /pubmed/34522608 http://dx.doi.org/10.1016/j.pacs.2021.100297 Text en © 2021 Published by Elsevier GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Matchynski, James I.
Manwar, Rayyan
Kratkiewicz, Karl J.
Madangopal, Rajtarun
Lennon, Veronica A.
Makki, Kassem M.
Reppen, Abbey L.
Woznicki, Alexander R.
Hope, Bruce T.
Perrine, Shane A.
Conti, Alana C.
Avanaki, Kamran
Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title_full Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title_fullStr Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title_full_unstemmed Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title_short Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study
title_sort direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated fos-lacz transgenic rat brain: a proof-of-principle study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426561/
https://www.ncbi.nlm.nih.gov/pubmed/34522608
http://dx.doi.org/10.1016/j.pacs.2021.100297
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