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Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury

Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxi...

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Autores principales: Watanabe, M., Borges, F.T., Pessoa, E.A., Fonseca, C.D., Fernandes, S.M., Drew, R.C., Volpini, R.A., Vattimo, M.F.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427747/
https://www.ncbi.nlm.nih.gov/pubmed/34495252
http://dx.doi.org/10.1590/1414-431X2021e9941
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author Watanabe, M.
Borges, F.T.
Pessoa, E.A.
Fonseca, C.D.
Fernandes, S.M.
Drew, R.C.
Volpini, R.A.
Vattimo, M.F.F.
author_facet Watanabe, M.
Borges, F.T.
Pessoa, E.A.
Fonseca, C.D.
Fernandes, S.M.
Drew, R.C.
Volpini, R.A.
Vattimo, M.F.F.
author_sort Watanabe, M.
collection PubMed
description Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect.
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spelling pubmed-84277472021-09-14 Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury Watanabe, M. Borges, F.T. Pessoa, E.A. Fonseca, C.D. Fernandes, S.M. Drew, R.C. Volpini, R.A. Vattimo, M.F.F. Braz J Med Biol Res Research Article Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect. Associação Brasileira de Divulgação Científica 2021-09-03 /pmc/articles/PMC8427747/ /pubmed/34495252 http://dx.doi.org/10.1590/1414-431X2021e9941 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Watanabe, M.
Borges, F.T.
Pessoa, E.A.
Fonseca, C.D.
Fernandes, S.M.
Drew, R.C.
Volpini, R.A.
Vattimo, M.F.F.
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title_full Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title_fullStr Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title_full_unstemmed Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title_short Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
title_sort renoprotective effect of n-acetylcysteine depends upon the severity of the ischemia reperfusion injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427747/
https://www.ncbi.nlm.nih.gov/pubmed/34495252
http://dx.doi.org/10.1590/1414-431X2021e9941
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