Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids

We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of Arabidopsis thaliana plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two...

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Autores principales: Fedorchuk, Tatiana P., Kireeva, Inga A., Opanasenko, Vera K., Terentyev, Vasily V., Rudenko, Natalia N., Borisova-Mubarakshina, Maria M., Ivanov, Boris N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427869/
https://www.ncbi.nlm.nih.gov/pubmed/34512677
http://dx.doi.org/10.3389/fpls.2021.662082
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author Fedorchuk, Tatiana P.
Kireeva, Inga A.
Opanasenko, Vera K.
Terentyev, Vasily V.
Rudenko, Natalia N.
Borisova-Mubarakshina, Maria M.
Ivanov, Boris N.
author_facet Fedorchuk, Tatiana P.
Kireeva, Inga A.
Opanasenko, Vera K.
Terentyev, Vasily V.
Rudenko, Natalia N.
Borisova-Mubarakshina, Maria M.
Ivanov, Boris N.
author_sort Fedorchuk, Tatiana P.
collection PubMed
description We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of Arabidopsis thaliana plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two mutant lines lacking the gene encoding alpha carbonic anhydrase 5 (αCA5). Using mass spectrometry, we revealed the presence of αCA5 in stromal thylakoid membranes of wild-type plants. A possible mechanism of the photophosphorylation stimulation by bicarbonate that involves αCA5 is proposed.
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spelling pubmed-84278692021-09-10 Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids Fedorchuk, Tatiana P. Kireeva, Inga A. Opanasenko, Vera K. Terentyev, Vasily V. Rudenko, Natalia N. Borisova-Mubarakshina, Maria M. Ivanov, Boris N. Front Plant Sci Plant Science We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of Arabidopsis thaliana plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two mutant lines lacking the gene encoding alpha carbonic anhydrase 5 (αCA5). Using mass spectrometry, we revealed the presence of αCA5 in stromal thylakoid membranes of wild-type plants. A possible mechanism of the photophosphorylation stimulation by bicarbonate that involves αCA5 is proposed. Frontiers Media S.A. 2021-08-26 /pmc/articles/PMC8427869/ /pubmed/34512677 http://dx.doi.org/10.3389/fpls.2021.662082 Text en Copyright © 2021 Fedorchuk, Kireeva, Opanasenko, Terentyev, Rudenko, Borisova-Mubarakshina and Ivanov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fedorchuk, Tatiana P.
Kireeva, Inga A.
Opanasenko, Vera K.
Terentyev, Vasily V.
Rudenko, Natalia N.
Borisova-Mubarakshina, Maria M.
Ivanov, Boris N.
Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title_full Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title_fullStr Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title_full_unstemmed Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title_short Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids
title_sort alpha carbonic anhydrase 5 mediates stimulation of atp synthesis by bicarbonate in isolated arabidopsis thylakoids
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427869/
https://www.ncbi.nlm.nih.gov/pubmed/34512677
http://dx.doi.org/10.3389/fpls.2021.662082
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