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Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428655/ https://www.ncbi.nlm.nih.gov/pubmed/34499652 http://dx.doi.org/10.1371/journal.pone.0252849 |
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author | Wisnewski, Adam V. Redlich, Carrie A. Liu, Jian Kamath, Kathy Abad, Queenie-Ann Smith, Richard F. Fazen, Louis Santiago, Romero Campillo Luna, Julian Martinez, Brian Baum-Jones, Elizabeth Waitz, Rebecca Haynes, Winston A. Shon, John C. |
author_facet | Wisnewski, Adam V. Redlich, Carrie A. Liu, Jian Kamath, Kathy Abad, Queenie-Ann Smith, Richard F. Fazen, Louis Santiago, Romero Campillo Luna, Julian Martinez, Brian Baum-Jones, Elizabeth Waitz, Rebecca Haynes, Winston A. Shon, John C. |
author_sort | Wisnewski, Adam V. |
collection | PubMed |
description | Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which may mimic 3-dimensional conformation (mimotopes). Dominant linear epitopes were identified in the C-terminal domains of the S1 and S2 subunits (aa 558–569, 627–638, and 1148–1159) which have been previously associated with SARS-CoV-2 neutralization in vitro and demonstrate identity to bat coronavirus and SARS-CoV, but limited homology to non-pathogenic human coronavirus. The identified COVID-19 mRNA vaccine epitopes should be considered in the context of variants, immune escape and vaccine and therapy design moving forward. |
format | Online Article Text |
id | pubmed-8428655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-84286552021-09-10 Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines Wisnewski, Adam V. Redlich, Carrie A. Liu, Jian Kamath, Kathy Abad, Queenie-Ann Smith, Richard F. Fazen, Louis Santiago, Romero Campillo Luna, Julian Martinez, Brian Baum-Jones, Elizabeth Waitz, Rebecca Haynes, Winston A. Shon, John C. PLoS One Research Article Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which may mimic 3-dimensional conformation (mimotopes). Dominant linear epitopes were identified in the C-terminal domains of the S1 and S2 subunits (aa 558–569, 627–638, and 1148–1159) which have been previously associated with SARS-CoV-2 neutralization in vitro and demonstrate identity to bat coronavirus and SARS-CoV, but limited homology to non-pathogenic human coronavirus. The identified COVID-19 mRNA vaccine epitopes should be considered in the context of variants, immune escape and vaccine and therapy design moving forward. Public Library of Science 2021-09-09 /pmc/articles/PMC8428655/ /pubmed/34499652 http://dx.doi.org/10.1371/journal.pone.0252849 Text en © 2021 Wisnewski et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wisnewski, Adam V. Redlich, Carrie A. Liu, Jian Kamath, Kathy Abad, Queenie-Ann Smith, Richard F. Fazen, Louis Santiago, Romero Campillo Luna, Julian Martinez, Brian Baum-Jones, Elizabeth Waitz, Rebecca Haynes, Winston A. Shon, John C. Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title | Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title_full | Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title_fullStr | Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title_full_unstemmed | Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title_short | Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines |
title_sort | immunogenic amino acid motifs and linear epitopes of covid-19 mrna vaccines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428655/ https://www.ncbi.nlm.nih.gov/pubmed/34499652 http://dx.doi.org/10.1371/journal.pone.0252849 |
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