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Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines

Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG...

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Autores principales: Wisnewski, Adam V., Redlich, Carrie A., Liu, Jian, Kamath, Kathy, Abad, Queenie-Ann, Smith, Richard F., Fazen, Louis, Santiago, Romero, Campillo Luna, Julian, Martinez, Brian, Baum-Jones, Elizabeth, Waitz, Rebecca, Haynes, Winston A., Shon, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428655/
https://www.ncbi.nlm.nih.gov/pubmed/34499652
http://dx.doi.org/10.1371/journal.pone.0252849
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author Wisnewski, Adam V.
Redlich, Carrie A.
Liu, Jian
Kamath, Kathy
Abad, Queenie-Ann
Smith, Richard F.
Fazen, Louis
Santiago, Romero
Campillo Luna, Julian
Martinez, Brian
Baum-Jones, Elizabeth
Waitz, Rebecca
Haynes, Winston A.
Shon, John C.
author_facet Wisnewski, Adam V.
Redlich, Carrie A.
Liu, Jian
Kamath, Kathy
Abad, Queenie-Ann
Smith, Richard F.
Fazen, Louis
Santiago, Romero
Campillo Luna, Julian
Martinez, Brian
Baum-Jones, Elizabeth
Waitz, Rebecca
Haynes, Winston A.
Shon, John C.
author_sort Wisnewski, Adam V.
collection PubMed
description Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which may mimic 3-dimensional conformation (mimotopes). Dominant linear epitopes were identified in the C-terminal domains of the S1 and S2 subunits (aa 558–569, 627–638, and 1148–1159) which have been previously associated with SARS-CoV-2 neutralization in vitro and demonstrate identity to bat coronavirus and SARS-CoV, but limited homology to non-pathogenic human coronavirus. The identified COVID-19 mRNA vaccine epitopes should be considered in the context of variants, immune escape and vaccine and therapy design moving forward.
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spelling pubmed-84286552021-09-10 Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines Wisnewski, Adam V. Redlich, Carrie A. Liu, Jian Kamath, Kathy Abad, Queenie-Ann Smith, Richard F. Fazen, Louis Santiago, Romero Campillo Luna, Julian Martinez, Brian Baum-Jones, Elizabeth Waitz, Rebecca Haynes, Winston A. Shon, John C. PLoS One Research Article Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which may mimic 3-dimensional conformation (mimotopes). Dominant linear epitopes were identified in the C-terminal domains of the S1 and S2 subunits (aa 558–569, 627–638, and 1148–1159) which have been previously associated with SARS-CoV-2 neutralization in vitro and demonstrate identity to bat coronavirus and SARS-CoV, but limited homology to non-pathogenic human coronavirus. The identified COVID-19 mRNA vaccine epitopes should be considered in the context of variants, immune escape and vaccine and therapy design moving forward. Public Library of Science 2021-09-09 /pmc/articles/PMC8428655/ /pubmed/34499652 http://dx.doi.org/10.1371/journal.pone.0252849 Text en © 2021 Wisnewski et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wisnewski, Adam V.
Redlich, Carrie A.
Liu, Jian
Kamath, Kathy
Abad, Queenie-Ann
Smith, Richard F.
Fazen, Louis
Santiago, Romero
Campillo Luna, Julian
Martinez, Brian
Baum-Jones, Elizabeth
Waitz, Rebecca
Haynes, Winston A.
Shon, John C.
Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title_full Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title_fullStr Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title_full_unstemmed Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title_short Immunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines
title_sort immunogenic amino acid motifs and linear epitopes of covid-19 mrna vaccines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428655/
https://www.ncbi.nlm.nih.gov/pubmed/34499652
http://dx.doi.org/10.1371/journal.pone.0252849
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