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Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis

PURPOSE: To investigate the effect of ferulic acid (FA) on spinal cord injury (SCI)-induced motor dysfunction and to explore the possible pharmacological mechanisms. METHODS: Adult male Wistar rats were used in our study. SCI was achieved by clipping the spinal cord T9 of the rat by a vascular clip...

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Autores principales: Jiang, Xi, Yu, Xuefeng, Chen, Jin, Jing, Changfeng, Xu, Lexing, Chen, Ziwei, Liu, Fuhe, Chen, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428671/
https://www.ncbi.nlm.nih.gov/pubmed/34495140
http://dx.doi.org/10.1590/ACB360705
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author Jiang, Xi
Yu, Xuefeng
Chen, Jin
Jing, Changfeng
Xu, Lexing
Chen, Ziwei
Liu, Fuhe
Chen, Lei
author_facet Jiang, Xi
Yu, Xuefeng
Chen, Jin
Jing, Changfeng
Xu, Lexing
Chen, Ziwei
Liu, Fuhe
Chen, Lei
author_sort Jiang, Xi
collection PubMed
description PURPOSE: To investigate the effect of ferulic acid (FA) on spinal cord injury (SCI)-induced motor dysfunction and to explore the possible pharmacological mechanisms. METHODS: Adult male Wistar rats were used in our study. SCI was achieved by clipping the spinal cord T9 of the rat by a vascular clip for 2 minutes. The motor function of the rat was evaluated by Basso, Beattie, and Bresnahan scoring method (BBB) and inclined plane test. Hematoxylin and eosin (HE) staining, NISSL staining, and transmission electron microscopic examination were used to evaluate alterations at the histological level. Polymerase chain reaction (PCR), Western blots, and enzyme-linked immunosorbent assays (ELISA) were employed in biochemical analysis. RESULTS: The BBB score and inclined plane test score significantly decreased after SCI surgery, whereas chronic FA treatment (dose of 90 mg/kg, i.g.) for 28 days improved SCI-induced motor dysfunction. HE staining showed that SCI surgery induced internal spinal cord edema, but the structural changes of the spinal cord could be reversed by FA treatment. NISSL staining and transmission electron microscopic examination confirmed the improvement of the effect of FA on the injury site. In the biochemical analysis, it could be found that FA inhibitedSCI-induced mRNA and protein overexpression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), as well as iNOS and COX-2 via the modulation of NF-κB level in the spinal cord of SCI rat. Moreover, the SCI-induced decrease of Bcl-2/Bax ratio was also reversed by FA treatment. However, the effect of FA on the expression of Beclin-1 was not statistically significant. CONCLUSIONS: FA showed a therapeutic effect on SCI, which may be associated with the regulation of neuroinflammation and apoptosis.
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spelling pubmed-84286712021-09-16 Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis Jiang, Xi Yu, Xuefeng Chen, Jin Jing, Changfeng Xu, Lexing Chen, Ziwei Liu, Fuhe Chen, Lei Acta Cir Bras Original Article PURPOSE: To investigate the effect of ferulic acid (FA) on spinal cord injury (SCI)-induced motor dysfunction and to explore the possible pharmacological mechanisms. METHODS: Adult male Wistar rats were used in our study. SCI was achieved by clipping the spinal cord T9 of the rat by a vascular clip for 2 minutes. The motor function of the rat was evaluated by Basso, Beattie, and Bresnahan scoring method (BBB) and inclined plane test. Hematoxylin and eosin (HE) staining, NISSL staining, and transmission electron microscopic examination were used to evaluate alterations at the histological level. Polymerase chain reaction (PCR), Western blots, and enzyme-linked immunosorbent assays (ELISA) were employed in biochemical analysis. RESULTS: The BBB score and inclined plane test score significantly decreased after SCI surgery, whereas chronic FA treatment (dose of 90 mg/kg, i.g.) for 28 days improved SCI-induced motor dysfunction. HE staining showed that SCI surgery induced internal spinal cord edema, but the structural changes of the spinal cord could be reversed by FA treatment. NISSL staining and transmission electron microscopic examination confirmed the improvement of the effect of FA on the injury site. In the biochemical analysis, it could be found that FA inhibitedSCI-induced mRNA and protein overexpression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), as well as iNOS and COX-2 via the modulation of NF-κB level in the spinal cord of SCI rat. Moreover, the SCI-induced decrease of Bcl-2/Bax ratio was also reversed by FA treatment. However, the effect of FA on the expression of Beclin-1 was not statistically significant. CONCLUSIONS: FA showed a therapeutic effect on SCI, which may be associated with the regulation of neuroinflammation and apoptosis. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021-09-03 /pmc/articles/PMC8428671/ /pubmed/34495140 http://dx.doi.org/10.1590/ACB360705 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jiang, Xi
Yu, Xuefeng
Chen, Jin
Jing, Changfeng
Xu, Lexing
Chen, Ziwei
Liu, Fuhe
Chen, Lei
Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title_full Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title_fullStr Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title_full_unstemmed Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title_short Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
title_sort ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428671/
https://www.ncbi.nlm.nih.gov/pubmed/34495140
http://dx.doi.org/10.1590/ACB360705
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