Cargando…

An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial

OBJECTIVE: This study conducted exploratory metabolomic and lipidomic profiling of plasma samples from the DASH (Dietary Approaches to Stop Hypertension) Sodium Trial to identify unique plasma biomarkers to identify salt-sensitive versus salt-resistant participants. METHODS: Utilizing plasma samples...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaudhary, Parul, Velkoska, Elena, Wainford, Richard D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429079/
https://www.ncbi.nlm.nih.gov/pubmed/34001808
http://dx.doi.org/10.1097/HJH.0000000000002904
_version_ 1783750493513711616
author Chaudhary, Parul
Velkoska, Elena
Wainford, Richard D.
author_facet Chaudhary, Parul
Velkoska, Elena
Wainford, Richard D.
author_sort Chaudhary, Parul
collection PubMed
description OBJECTIVE: This study conducted exploratory metabolomic and lipidomic profiling of plasma samples from the DASH (Dietary Approaches to Stop Hypertension) Sodium Trial to identify unique plasma biomarkers to identify salt-sensitive versus salt-resistant participants. METHODS: Utilizing plasma samples from the DASH-Sodium Trial, we conducted untargeted metabolomic and lipidomic profiling on plasma from salt-sensitive and salt-resistant DASH-Sodium Trial participants. Study 1 analyzed plasma from 106 salt-sensitive and 85 salt-resistant participants obtained during screening when participants consumed their regular diet. Study 2 examined paired within-participant plasma samples in 20 salt-sensitive and 20 salt-resistant participants during a high-salt and low-salt dietary intervention. To investigate differences in metabolites or lipidomes that could discriminate between salt-sensitive and salt-resistant participants or the response to a dietary sodium intervention Principal Component Analysis and Orthogonal Partial Least Square Discriminant Analysis was conducted. Differential expression analysis was performed to validate observed variance and to determine the statistical significance. RESULTS: Differential expression analysis between salt-sensitive and salt-resistant participants at screening revealed no difference in plasma metabolites or lipidomes. In contrast, three annotated plasma metabolites, tocopherol alpha, 2-ketoisocaproic acid, and citramalic acid, differed significantly between high-sodium and low-sodium dietary interventions in salt-sensitive participants. CONCLUSION: In DASH-Sodium Trial participants on a regular diet, plasma metabolomic or lipidomic signatures were not different between salt-sensitive and salt-resistant participants. High-sodium intake was associated with changes in specific circulating metabolites in salt-sensitive participants. Further studies are needed to validate the identified metabolites as potential biomarkers that are associated with the salt sensitivity of blood pressure.
format Online
Article
Text
id pubmed-8429079
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-84290792021-09-28 An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial Chaudhary, Parul Velkoska, Elena Wainford, Richard D. J Hypertens ORIGINAL PAPERS: Diagnostics OBJECTIVE: This study conducted exploratory metabolomic and lipidomic profiling of plasma samples from the DASH (Dietary Approaches to Stop Hypertension) Sodium Trial to identify unique plasma biomarkers to identify salt-sensitive versus salt-resistant participants. METHODS: Utilizing plasma samples from the DASH-Sodium Trial, we conducted untargeted metabolomic and lipidomic profiling on plasma from salt-sensitive and salt-resistant DASH-Sodium Trial participants. Study 1 analyzed plasma from 106 salt-sensitive and 85 salt-resistant participants obtained during screening when participants consumed their regular diet. Study 2 examined paired within-participant plasma samples in 20 salt-sensitive and 20 salt-resistant participants during a high-salt and low-salt dietary intervention. To investigate differences in metabolites or lipidomes that could discriminate between salt-sensitive and salt-resistant participants or the response to a dietary sodium intervention Principal Component Analysis and Orthogonal Partial Least Square Discriminant Analysis was conducted. Differential expression analysis was performed to validate observed variance and to determine the statistical significance. RESULTS: Differential expression analysis between salt-sensitive and salt-resistant participants at screening revealed no difference in plasma metabolites or lipidomes. In contrast, three annotated plasma metabolites, tocopherol alpha, 2-ketoisocaproic acid, and citramalic acid, differed significantly between high-sodium and low-sodium dietary interventions in salt-sensitive participants. CONCLUSION: In DASH-Sodium Trial participants on a regular diet, plasma metabolomic or lipidomic signatures were not different between salt-sensitive and salt-resistant participants. High-sodium intake was associated with changes in specific circulating metabolites in salt-sensitive participants. Further studies are needed to validate the identified metabolites as potential biomarkers that are associated with the salt sensitivity of blood pressure. Lippincott Williams & Wilkins 2021-10 2021-05-17 /pmc/articles/PMC8429079/ /pubmed/34001808 http://dx.doi.org/10.1097/HJH.0000000000002904 Text en Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle ORIGINAL PAPERS: Diagnostics
Chaudhary, Parul
Velkoska, Elena
Wainford, Richard D.
An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title_full An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title_fullStr An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title_full_unstemmed An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title_short An exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from The Dietary Approaches to Stop Hypertension Sodium Trial
title_sort exploratory analysis of comparative plasma metabolomic and lipidomic profiling in salt-sensitive and salt-resistant individuals from the dietary approaches to stop hypertension sodium trial
topic ORIGINAL PAPERS: Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429079/
https://www.ncbi.nlm.nih.gov/pubmed/34001808
http://dx.doi.org/10.1097/HJH.0000000000002904
work_keys_str_mv AT chaudharyparul anexploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial
AT velkoskaelena anexploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial
AT wainfordrichardd anexploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial
AT chaudharyparul exploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial
AT velkoskaelena exploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial
AT wainfordrichardd exploratoryanalysisofcomparativeplasmametabolomicandlipidomicprofilinginsaltsensitiveandsaltresistantindividualsfromthedietaryapproachestostophypertensionsodiumtrial