Cargando…

Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune

Different types of multinucleated giant cells (MGCs) of myeloid origin have been described; osteoclasts are the most extensively studied because of their importance in bone homeostasis. MGCs are formed by cell-to-cell fusion, and most types have been observed in pathological conditions, especially i...

Descripción completa

Detalles Bibliográficos
Autores principales: Dufrançais, Ophélie, Mascarau, Rémi, Poincloux, Renaud, Maridonneau-Parini, Isabelle, Raynaud-Messina, Brigitte, Vérollet, Christel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429379/
https://www.ncbi.nlm.nih.gov/pubmed/34296319
http://dx.doi.org/10.1007/s00018-021-03875-x
_version_ 1783750518652272640
author Dufrançais, Ophélie
Mascarau, Rémi
Poincloux, Renaud
Maridonneau-Parini, Isabelle
Raynaud-Messina, Brigitte
Vérollet, Christel
author_facet Dufrançais, Ophélie
Mascarau, Rémi
Poincloux, Renaud
Maridonneau-Parini, Isabelle
Raynaud-Messina, Brigitte
Vérollet, Christel
author_sort Dufrançais, Ophélie
collection PubMed
description Different types of multinucleated giant cells (MGCs) of myeloid origin have been described; osteoclasts are the most extensively studied because of their importance in bone homeostasis. MGCs are formed by cell-to-cell fusion, and most types have been observed in pathological conditions, especially in infectious and non-infectious chronic inflammatory contexts. The precise role of the different MGCs and the mechanisms that govern their formation remain poorly understood, likely due to their heterogeneity. First, we will introduce the main populations of MGCs derived from the monocyte/macrophage lineage. We will then discuss the known molecular actors mediating the early stages of fusion, focusing on cell-surface receptors involved in the cell-to-cell adhesion steps that ultimately lead to multinucleation. Given that cell-to-cell fusion is a complex and well-coordinated process, we will also describe what is currently known about the evolution of F-actin-based structures involved in macrophage fusion, i.e., podosomes, zipper-like structures, and tunneling nanotubes (TNT). Finally, the localization and potential role of the key fusion mediators related to the formation of these F-actin structures will be discussed. This review intends to present the current status of knowledge of the molecular and cellular mechanisms supporting multinucleation of myeloid cells, highlighting the gaps still existing, and contributing to the proposition of potential disease-specific MGC markers and/or therapeutic targets.
format Online
Article
Text
id pubmed-8429379
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-84293792021-09-29 Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune Dufrançais, Ophélie Mascarau, Rémi Poincloux, Renaud Maridonneau-Parini, Isabelle Raynaud-Messina, Brigitte Vérollet, Christel Cell Mol Life Sci Review Different types of multinucleated giant cells (MGCs) of myeloid origin have been described; osteoclasts are the most extensively studied because of their importance in bone homeostasis. MGCs are formed by cell-to-cell fusion, and most types have been observed in pathological conditions, especially in infectious and non-infectious chronic inflammatory contexts. The precise role of the different MGCs and the mechanisms that govern their formation remain poorly understood, likely due to their heterogeneity. First, we will introduce the main populations of MGCs derived from the monocyte/macrophage lineage. We will then discuss the known molecular actors mediating the early stages of fusion, focusing on cell-surface receptors involved in the cell-to-cell adhesion steps that ultimately lead to multinucleation. Given that cell-to-cell fusion is a complex and well-coordinated process, we will also describe what is currently known about the evolution of F-actin-based structures involved in macrophage fusion, i.e., podosomes, zipper-like structures, and tunneling nanotubes (TNT). Finally, the localization and potential role of the key fusion mediators related to the formation of these F-actin structures will be discussed. This review intends to present the current status of knowledge of the molecular and cellular mechanisms supporting multinucleation of myeloid cells, highlighting the gaps still existing, and contributing to the proposition of potential disease-specific MGC markers and/or therapeutic targets. Springer International Publishing 2021-07-23 2021 /pmc/articles/PMC8429379/ /pubmed/34296319 http://dx.doi.org/10.1007/s00018-021-03875-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Dufrançais, Ophélie
Mascarau, Rémi
Poincloux, Renaud
Maridonneau-Parini, Isabelle
Raynaud-Messina, Brigitte
Vérollet, Christel
Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title_full Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title_fullStr Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title_full_unstemmed Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title_short Cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
title_sort cellular and molecular actors of myeloid cell fusion: podosomes and tunneling nanotubes call the tune
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429379/
https://www.ncbi.nlm.nih.gov/pubmed/34296319
http://dx.doi.org/10.1007/s00018-021-03875-x
work_keys_str_mv AT dufrancaisophelie cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune
AT mascarauremi cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune
AT poinclouxrenaud cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune
AT maridonneaupariniisabelle cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune
AT raynaudmessinabrigitte cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune
AT verolletchristel cellularandmolecularactorsofmyeloidcellfusionpodosomesandtunnelingnanotubescallthetune