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Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells

Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas mo...

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Autores principales: Dahlrot, Rikke H., Bangsø, Julie A., Petersen, Jeanette K., Rosager, Ann Mari, Sørensen, Mia D., Reifenberger, Guido, Hansen, Steinbjørn, Kristensen, Bjarne W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429554/
https://www.ncbi.nlm.nih.gov/pubmed/34504133
http://dx.doi.org/10.1038/s41598-021-95958-9
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author Dahlrot, Rikke H.
Bangsø, Julie A.
Petersen, Jeanette K.
Rosager, Ann Mari
Sørensen, Mia D.
Reifenberger, Guido
Hansen, Steinbjørn
Kristensen, Bjarne W.
author_facet Dahlrot, Rikke H.
Bangsø, Julie A.
Petersen, Jeanette K.
Rosager, Ann Mari
Sørensen, Mia D.
Reifenberger, Guido
Hansen, Steinbjørn
Kristensen, Bjarne W.
author_sort Dahlrot, Rikke H.
collection PubMed
description Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas more precisely than previously by excluding proliferation in non-tumor cells from the analysis. We investigated the Ki-67 LI in a well-annotated population-based glioblastoma patient cohort (178 IDH-wildtype, 3 IDH-mutated). Ki-67 was identified in full tumor sections with automated digital image analysis and the contribution from non-tumor cells was excluded using quantitative double-immunohistochemistry. For comparison of the Ki-67 LI between WHO grades (II-IV), 9 IDH-mutated diffuse astrocytomas and 9 IDH-mutated anaplastic astrocytomas were stained. Median Ki-67 LI increased with increasing WHO grade (median 2.7%, 6.4% and 27.5%). There was no difference in median Ki-67 LI between IDH-mutated and IDH-wildtype glioblastomas (p = 0.9) and Ki-67 LI was not associated with survival in glioblastomas in neither univariate (p = 0.9) nor multivariate analysis including MGMT promoter methylation status and excluding IDH-mutated glioblastomas (p = 0.2). Ki-67 may be of value in the differential diagnostic setting, but it must not be over-interpreted in the clinico-pathological context.
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spelling pubmed-84295542021-09-10 Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells Dahlrot, Rikke H. Bangsø, Julie A. Petersen, Jeanette K. Rosager, Ann Mari Sørensen, Mia D. Reifenberger, Guido Hansen, Steinbjørn Kristensen, Bjarne W. Sci Rep Article Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas more precisely than previously by excluding proliferation in non-tumor cells from the analysis. We investigated the Ki-67 LI in a well-annotated population-based glioblastoma patient cohort (178 IDH-wildtype, 3 IDH-mutated). Ki-67 was identified in full tumor sections with automated digital image analysis and the contribution from non-tumor cells was excluded using quantitative double-immunohistochemistry. For comparison of the Ki-67 LI between WHO grades (II-IV), 9 IDH-mutated diffuse astrocytomas and 9 IDH-mutated anaplastic astrocytomas were stained. Median Ki-67 LI increased with increasing WHO grade (median 2.7%, 6.4% and 27.5%). There was no difference in median Ki-67 LI between IDH-mutated and IDH-wildtype glioblastomas (p = 0.9) and Ki-67 LI was not associated with survival in glioblastomas in neither univariate (p = 0.9) nor multivariate analysis including MGMT promoter methylation status and excluding IDH-mutated glioblastomas (p = 0.2). Ki-67 may be of value in the differential diagnostic setting, but it must not be over-interpreted in the clinico-pathological context. Nature Publishing Group UK 2021-09-09 /pmc/articles/PMC8429554/ /pubmed/34504133 http://dx.doi.org/10.1038/s41598-021-95958-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dahlrot, Rikke H.
Bangsø, Julie A.
Petersen, Jeanette K.
Rosager, Ann Mari
Sørensen, Mia D.
Reifenberger, Guido
Hansen, Steinbjørn
Kristensen, Bjarne W.
Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_full Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_fullStr Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_full_unstemmed Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_short Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_sort prognostic role of ki-67 in glioblastomas excluding contribution from non-neoplastic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429554/
https://www.ncbi.nlm.nih.gov/pubmed/34504133
http://dx.doi.org/10.1038/s41598-021-95958-9
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