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Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429709/ https://www.ncbi.nlm.nih.gov/pubmed/34504247 http://dx.doi.org/10.1038/s41598-021-97460-8 |
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author | Kodama, Joe Harumningtyas, Anjar Anggraini Ito, Tomoko Michlíček, Miroslav Sugimoto, Satoshi Kita, Hidekazu Chijimatsu, Ryota Ukon, Yuichiro Kushioka, Junichi Okada, Rintaro Kamatani, Takashi Hashimoto, Kunihiko Tateiwa, Daisuke Tsukazaki, Hiroyuki Nakagawa, Shinichi Takenaka, Shota Makino, Takahiro Sakai, Yusuke Nečas, David Zajíčková, Lenka Hamaguchi, Satoshi Kaito, Takashi |
author_facet | Kodama, Joe Harumningtyas, Anjar Anggraini Ito, Tomoko Michlíček, Miroslav Sugimoto, Satoshi Kita, Hidekazu Chijimatsu, Ryota Ukon, Yuichiro Kushioka, Junichi Okada, Rintaro Kamatani, Takashi Hashimoto, Kunihiko Tateiwa, Daisuke Tsukazaki, Hiroyuki Nakagawa, Shinichi Takenaka, Shota Makino, Takahiro Sakai, Yusuke Nečas, David Zajíčková, Lenka Hamaguchi, Satoshi Kaito, Takashi |
author_sort | Kodama, Joe |
collection | PubMed |
description | Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH(4)/N(2)/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone. |
format | Online Article Text |
id | pubmed-8429709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84297092021-09-13 Amine modification of calcium phosphate by low-pressure plasma for bone regeneration Kodama, Joe Harumningtyas, Anjar Anggraini Ito, Tomoko Michlíček, Miroslav Sugimoto, Satoshi Kita, Hidekazu Chijimatsu, Ryota Ukon, Yuichiro Kushioka, Junichi Okada, Rintaro Kamatani, Takashi Hashimoto, Kunihiko Tateiwa, Daisuke Tsukazaki, Hiroyuki Nakagawa, Shinichi Takenaka, Shota Makino, Takahiro Sakai, Yusuke Nečas, David Zajíčková, Lenka Hamaguchi, Satoshi Kaito, Takashi Sci Rep Article Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH(4)/N(2)/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone. Nature Publishing Group UK 2021-09-09 /pmc/articles/PMC8429709/ /pubmed/34504247 http://dx.doi.org/10.1038/s41598-021-97460-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kodama, Joe Harumningtyas, Anjar Anggraini Ito, Tomoko Michlíček, Miroslav Sugimoto, Satoshi Kita, Hidekazu Chijimatsu, Ryota Ukon, Yuichiro Kushioka, Junichi Okada, Rintaro Kamatani, Takashi Hashimoto, Kunihiko Tateiwa, Daisuke Tsukazaki, Hiroyuki Nakagawa, Shinichi Takenaka, Shota Makino, Takahiro Sakai, Yusuke Nečas, David Zajíčková, Lenka Hamaguchi, Satoshi Kaito, Takashi Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title | Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title_full | Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title_fullStr | Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title_full_unstemmed | Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title_short | Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
title_sort | amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429709/ https://www.ncbi.nlm.nih.gov/pubmed/34504247 http://dx.doi.org/10.1038/s41598-021-97460-8 |
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