Cargando…

Amine modification of calcium phosphate by low-pressure plasma for bone regeneration

Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is...

Descripción completa

Detalles Bibliográficos
Autores principales: Kodama, Joe, Harumningtyas, Anjar Anggraini, Ito, Tomoko, Michlíček, Miroslav, Sugimoto, Satoshi, Kita, Hidekazu, Chijimatsu, Ryota, Ukon, Yuichiro, Kushioka, Junichi, Okada, Rintaro, Kamatani, Takashi, Hashimoto, Kunihiko, Tateiwa, Daisuke, Tsukazaki, Hiroyuki, Nakagawa, Shinichi, Takenaka, Shota, Makino, Takahiro, Sakai, Yusuke, Nečas, David, Zajíčková, Lenka, Hamaguchi, Satoshi, Kaito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429709/
https://www.ncbi.nlm.nih.gov/pubmed/34504247
http://dx.doi.org/10.1038/s41598-021-97460-8
_version_ 1783750586754138112
author Kodama, Joe
Harumningtyas, Anjar Anggraini
Ito, Tomoko
Michlíček, Miroslav
Sugimoto, Satoshi
Kita, Hidekazu
Chijimatsu, Ryota
Ukon, Yuichiro
Kushioka, Junichi
Okada, Rintaro
Kamatani, Takashi
Hashimoto, Kunihiko
Tateiwa, Daisuke
Tsukazaki, Hiroyuki
Nakagawa, Shinichi
Takenaka, Shota
Makino, Takahiro
Sakai, Yusuke
Nečas, David
Zajíčková, Lenka
Hamaguchi, Satoshi
Kaito, Takashi
author_facet Kodama, Joe
Harumningtyas, Anjar Anggraini
Ito, Tomoko
Michlíček, Miroslav
Sugimoto, Satoshi
Kita, Hidekazu
Chijimatsu, Ryota
Ukon, Yuichiro
Kushioka, Junichi
Okada, Rintaro
Kamatani, Takashi
Hashimoto, Kunihiko
Tateiwa, Daisuke
Tsukazaki, Hiroyuki
Nakagawa, Shinichi
Takenaka, Shota
Makino, Takahiro
Sakai, Yusuke
Nečas, David
Zajíčková, Lenka
Hamaguchi, Satoshi
Kaito, Takashi
author_sort Kodama, Joe
collection PubMed
description Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH(4)/N(2)/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.
format Online
Article
Text
id pubmed-8429709
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-84297092021-09-13 Amine modification of calcium phosphate by low-pressure plasma for bone regeneration Kodama, Joe Harumningtyas, Anjar Anggraini Ito, Tomoko Michlíček, Miroslav Sugimoto, Satoshi Kita, Hidekazu Chijimatsu, Ryota Ukon, Yuichiro Kushioka, Junichi Okada, Rintaro Kamatani, Takashi Hashimoto, Kunihiko Tateiwa, Daisuke Tsukazaki, Hiroyuki Nakagawa, Shinichi Takenaka, Shota Makino, Takahiro Sakai, Yusuke Nečas, David Zajíčková, Lenka Hamaguchi, Satoshi Kaito, Takashi Sci Rep Article Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH(4)/N(2)/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone. Nature Publishing Group UK 2021-09-09 /pmc/articles/PMC8429709/ /pubmed/34504247 http://dx.doi.org/10.1038/s41598-021-97460-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kodama, Joe
Harumningtyas, Anjar Anggraini
Ito, Tomoko
Michlíček, Miroslav
Sugimoto, Satoshi
Kita, Hidekazu
Chijimatsu, Ryota
Ukon, Yuichiro
Kushioka, Junichi
Okada, Rintaro
Kamatani, Takashi
Hashimoto, Kunihiko
Tateiwa, Daisuke
Tsukazaki, Hiroyuki
Nakagawa, Shinichi
Takenaka, Shota
Makino, Takahiro
Sakai, Yusuke
Nečas, David
Zajíčková, Lenka
Hamaguchi, Satoshi
Kaito, Takashi
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title_full Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title_fullStr Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title_full_unstemmed Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title_short Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
title_sort amine modification of calcium phosphate by low-pressure plasma for bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429709/
https://www.ncbi.nlm.nih.gov/pubmed/34504247
http://dx.doi.org/10.1038/s41598-021-97460-8
work_keys_str_mv AT kodamajoe aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT harumningtyasanjaranggraini aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT itotomoko aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT michlicekmiroslav aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT sugimotosatoshi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT kitahidekazu aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT chijimatsuryota aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT ukonyuichiro aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT kushiokajunichi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT okadarintaro aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT kamatanitakashi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT hashimotokunihiko aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT tateiwadaisuke aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT tsukazakihiroyuki aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT nakagawashinichi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT takenakashota aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT makinotakahiro aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT sakaiyusuke aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT necasdavid aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT zajickovalenka aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT hamaguchisatoshi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration
AT kaitotakashi aminemodificationofcalciumphosphatebylowpressureplasmaforboneregeneration