Cargando…
Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HA(TMN) neurons) has produced inconsis...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429727/ https://www.ncbi.nlm.nih.gov/pubmed/34504120 http://dx.doi.org/10.1038/s41598-021-95497-3 |
_version_ | 1783750590900207616 |
---|---|
author | Naganuma, Fumito Nakamura, Tadaho Kuroyanagi, Hiroshi Tanaka, Masato Yoshikawa, Takeo Yanai, Kazuhiko Okamura, Nobuyuki |
author_facet | Naganuma, Fumito Nakamura, Tadaho Kuroyanagi, Hiroshi Tanaka, Masato Yoshikawa, Takeo Yanai, Kazuhiko Okamura, Nobuyuki |
author_sort | Naganuma, Fumito |
collection | PubMed |
description | Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HA(TMN) neurons) has produced inconsistent effects on the sleep–wake cycle, possibly due to the use of Hdc-Cre mice driving Cre recombinase and DREADDs activity outside the targeted region. Moreover, previous DREADDs studies have not examined locomotor activity and aggressive behaviours, which are also regulated by brain histamine levels. In the present study, we investigated the effects of HA(TMN) activation and inhibition on the locomotor activity, aggressive behaviours and sleep–wake cycle of Hdc-Cre mice with minimal non-target expression of Cre-recombinase. Chemoactivation of HA(TMN) moderately enhanced locomotor activity in a novel open field. Activation of HA(TMN) neurons significantly enhanced aggressive behaviour in the resident–intruder test. Wakefulness was increased and non-rapid eye movement (NREM) sleep decreased for an hour by HA(TMN) chemoactivation. Conversely HA(TMN) chemoinhibition decreased wakefulness and increased NREM sleep for 6 h. These changes in wakefulness induced by HA(TMN) modulation were related to the maintenance of vigilance state. These results indicate the influences of HA(TMN) neurons on exploratory activity, territorial aggression, and wake maintenance. |
format | Online Article Text |
id | pubmed-8429727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84297272021-09-13 Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness Naganuma, Fumito Nakamura, Tadaho Kuroyanagi, Hiroshi Tanaka, Masato Yoshikawa, Takeo Yanai, Kazuhiko Okamura, Nobuyuki Sci Rep Article Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HA(TMN) neurons) has produced inconsistent effects on the sleep–wake cycle, possibly due to the use of Hdc-Cre mice driving Cre recombinase and DREADDs activity outside the targeted region. Moreover, previous DREADDs studies have not examined locomotor activity and aggressive behaviours, which are also regulated by brain histamine levels. In the present study, we investigated the effects of HA(TMN) activation and inhibition on the locomotor activity, aggressive behaviours and sleep–wake cycle of Hdc-Cre mice with minimal non-target expression of Cre-recombinase. Chemoactivation of HA(TMN) moderately enhanced locomotor activity in a novel open field. Activation of HA(TMN) neurons significantly enhanced aggressive behaviour in the resident–intruder test. Wakefulness was increased and non-rapid eye movement (NREM) sleep decreased for an hour by HA(TMN) chemoactivation. Conversely HA(TMN) chemoinhibition decreased wakefulness and increased NREM sleep for 6 h. These changes in wakefulness induced by HA(TMN) modulation were related to the maintenance of vigilance state. These results indicate the influences of HA(TMN) neurons on exploratory activity, territorial aggression, and wake maintenance. Nature Publishing Group UK 2021-09-09 /pmc/articles/PMC8429727/ /pubmed/34504120 http://dx.doi.org/10.1038/s41598-021-95497-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Naganuma, Fumito Nakamura, Tadaho Kuroyanagi, Hiroshi Tanaka, Masato Yoshikawa, Takeo Yanai, Kazuhiko Okamura, Nobuyuki Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title | Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title_full | Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title_fullStr | Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title_full_unstemmed | Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title_short | Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
title_sort | chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429727/ https://www.ncbi.nlm.nih.gov/pubmed/34504120 http://dx.doi.org/10.1038/s41598-021-95497-3 |
work_keys_str_mv | AT naganumafumito chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT nakamuratadaho chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT kuroyanagihiroshi chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT tanakamasato chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT yoshikawatakeo chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT yanaikazuhiko chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT okamuranobuyuki chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness |