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CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc

ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly reg...

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Autores principales: Jalal, Adam S.B., Tran, Ngat T., Wu, Ling J., Ramakrishnan, Karunakaran, Rejzek, Martin, Gobbato, Giulia, Stevenson, Clare E.M., Lawson, David M., Errington, Jeff, Le, Tung B.K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429893/
https://www.ncbi.nlm.nih.gov/pubmed/34270916
http://dx.doi.org/10.1016/j.molcel.2021.06.025
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author Jalal, Adam S.B.
Tran, Ngat T.
Wu, Ling J.
Ramakrishnan, Karunakaran
Rejzek, Martin
Gobbato, Giulia
Stevenson, Clare E.M.
Lawson, David M.
Errington, Jeff
Le, Tung B.K.
author_facet Jalal, Adam S.B.
Tran, Ngat T.
Wu, Ling J.
Ramakrishnan, Karunakaran
Rejzek, Martin
Gobbato, Giulia
Stevenson, Clare E.M.
Lawson, David M.
Errington, Jeff
Le, Tung B.K.
author_sort Jalal, Adam S.B.
collection PubMed
description ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity.
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spelling pubmed-84298932021-09-14 CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc Jalal, Adam S.B. Tran, Ngat T. Wu, Ling J. Ramakrishnan, Karunakaran Rejzek, Martin Gobbato, Giulia Stevenson, Clare E.M. Lawson, David M. Errington, Jeff Le, Tung B.K. Mol Cell Article ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity. Cell Press 2021-09-02 /pmc/articles/PMC8429893/ /pubmed/34270916 http://dx.doi.org/10.1016/j.molcel.2021.06.025 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jalal, Adam S.B.
Tran, Ngat T.
Wu, Ling J.
Ramakrishnan, Karunakaran
Rejzek, Martin
Gobbato, Giulia
Stevenson, Clare E.M.
Lawson, David M.
Errington, Jeff
Le, Tung B.K.
CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title_full CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title_fullStr CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title_full_unstemmed CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title_short CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
title_sort ctp regulates membrane-binding activity of the nucleoid occlusion protein noc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429893/
https://www.ncbi.nlm.nih.gov/pubmed/34270916
http://dx.doi.org/10.1016/j.molcel.2021.06.025
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